Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,470	C→T	noncoding (35/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,470	0	C	T	100.0%	83.7 / NA	28	noncoding (35/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/12);  total (16/12)

TCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTT  >  NC_000913/696332‑696604                                                                                                                                           |                                                                                                                                       tCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGaa                                                                                                                                    >  1:457225/1‑143 (MQ=255)  cgcgTCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAt                                                                                                                                   <  2:335121/143‑1 (MQ=255)      tCCGACATTGAAGGATAAGACGTGTCAACATCGCATTGGTGAATGAAAGAACGCACACCAGCAAACAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTCCGAGGATTCGCGCCTCGGACTGCCGGACTTAGAATCCgg                                                                                                                               >  2:348423/1‑143 (MQ=255)        cGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTCCGAGGATTCGAACCTCGGAATGCCGGAATTGGAATCCGGTg                                                                                                                             >  1:57366/1‑143 (MQ=255)         caCATTGAAGGATACGGCGTGTCAACATCTCATTCGACATTGAATGAACGCAGAAAAGCAAAATGCTCGGCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGc                                                                                                                            <  1:35300/142‑1 (MQ=37)                aaGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTGGGTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCg                                                                                                                     <  2:424540/143‑1 (MQ=255)                     tAAGACGGGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTgg                                                                                                                <  1:659070/143‑1 (MQ=255)                      aaGACGTGTCAACATCGCCTTCGCCATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGc                                                                                                               <  2:623467/143‑1 (MQ=255)                             gtCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTGGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCGCGGGATGCCGGGATTAGAATCCGGTGCCTTACCGCTTGGCGATAccc                                                                                                        >  1:348991/1‑143 (MQ=37)                             gtCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATAccc                                                                                                        >  1:506404/1‑143 (MQ=255)                                   aTCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAACTAGAATCGGGTGCCCCACCGCTCGGCCGGACCCCCACTg                                                                                                  >  2:87322/1‑143 (MQ=25)                                    tggCATTCGACGTGGAAGGAACGCGGAAAAGCAAGACGCTCGCCGAAGCGAGCTTTTGTAAGGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAGTCCGGTGCCTTACCGCTTGGCGATACCCCAACTgg                                                                                                 <  2:485403/141‑1 (MQ=18)                                    tCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTgg                                                                                                 >  1:287159/1‑143 (MQ=255)                                      gCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAAGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGt                                                                                               >  1:101092/1‑143 (MQ=255)                                        aTTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGCATTAAAATACCTTACCTTACCGCTTGGCGATACCCGAACAggcagc                                                                                             >  2:160613/1‑139 (MQ=25)                                         ttCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCa                                                                                            >  1:271324/1‑143 (MQ=255)                                                  gaatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACGGCTTGGCGATACCCCAACTGGGTGGACTTGCAAgg                                                                                   >  2:371101/1‑143 (MQ=255)                                                  gaatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAgg                                                                                   <  2:62470/143‑1 (MQ=255)                                                   aatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGGTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGt                                                                                  <  2:711724/143‑1 (MQ=255)                                                    atgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTTGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTAGGAGCACTTACAAggga                                                                                 >  2:578626/1‑141 (MQ=255)                                                       aaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGATAAGc                                                                              >  1:207638/1‑143 (MQ=255)                                                            aGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCtt                                                                         <  2:506404/143‑1 (MQ=255)                                                              aaaaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGa                                                                       <  2:287159/143‑1 (MQ=255)                                                                     aaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAtt                                                                >  1:579307/1‑143 (MQ=255)                                                                        aGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGc                                                             <  2:457225/143‑1 (MQ=255)                                                                                                               gAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAAGAAATTGGCTGGGGTACGATGATTCGAACCTCGGAATGCCGGTAtacg                      >  1:259472/1‑140 (MQ=37)                                                                                                                         cccTCGGAATGCCGTAATTAGAAGCCGATGCCTTACCGCGTGGCGAAACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc            <  1:397668/142‑1 (MQ=37)                                                                                                                                   gCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGCATCAGGATCCGGTGCCTTGCCGCtt  >  1:461632/1‑143 (MQ=255)                                                                                                                                           |                                                                                                                                       TCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTT  >  NC_000913/696332‑696604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑