Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 759,250 G→T R182L (CGC→CTC)  sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913759,2500GT97.1% 83.4 / NA 35R182L (CGC→CTC) sucAsubunit of E1(0) component of 2‑oxoglutarate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (22/12);  minor base A (1/0);  total (23/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTC  >  NC_000913/759110‑759388
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ccAGGGGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAActct                                                                                                                                          <  1:369979/143‑1 (MQ=255)
ccAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAActct                                                                                                                                          <  1:92578/143‑1 (MQ=255)
    gagaCCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGCAAAACTCTGGAt                                                                                                                                      >  2:140583/1‑143 (MQ=255)
          ttCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACAATGAAAATAGGCGAGCTGCTGGAAGCCCTCAAGAACACCTACTGCGGCCCGATTGGTGCCGAGGATATGCACATTACCAGCACCGAAGAACAACTGCGGATCAAACa                                                                                                                                >  2:638175/1‑143 (MQ=255)
                     gtCATGTGCCAGCGGCAAAGAAAAGATGGAGTTTTGCGAGCTTCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGt                                                                                                                     <  1:736401/142‑1 (MQ=255)
                      tCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAACACTCTGGAGCCAACAGCGTATCGAGTc                                                                                                                    >  1:24666/1‑143 (MQ=255)
                        aTTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCCCCGAAGAAAAACTCTGGCTCCAACAGCGTATCGAGTCTg                                                                                                                  >  1:343503/1‑143 (MQ=255)
                         tttGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGCGTATATGCACATTACCAGCACCGAAGACACACTCTGGATCCCACAGCGTAGCGAGCCTgg                                                                                                                 >  2:436807/1‑143 (MQ=255)
                         tttGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGCGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATGCAACAGCGTAACGCGTCTgg                                                                                                                 >  1:597230/1‑143 (MQ=255)
                             ccAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCGGGATCCAACAGCGTAGCGAGTCTgatggc                                                                                                             >  2:242480/1‑138 (MQ=255)
                              cAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGGATCGAGTCTGGGcgcg                                                                                                            >  2:633203/1‑143 (MQ=255)
                              cAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACACTGGATCCAACAGCGGATCGAGGCTGGTcgcg                                                                                                            >  2:326533/1‑143 (MQ=255)
                              cAGCGGCAAAGAAACCATGAAACTCGGCGAGCGGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTcgcg                                                                                                            <  1:140583/143‑1 (MQ=255)
                                    cAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACtt                                                                                                      >  1:535379/1‑143 (MQ=255)
                                               tGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAActct                                                                                                                                          <  1:642738/96‑1 (MQ=255)
                                               tGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAActct                                                                                                                                          >  2:642738/1‑96 (MQ=255)
                                                   aCTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCGGCACCGAAGAAAAACTCTGGATCCAACAGCGTATAGAGCATGGTCGCCCGACTCTCAATAACCACgaga                                                                                       >  2:154334/1‑143 (MQ=255)
                                                   aCTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTGTCAATAGCGAAgaga                                                                                       >  2:462239/1‑143 (MQ=255)
                                                             ctgctgGATGCACGCAAGCAACCCGACTGCGGCCCGATTGGGGCCGAGTATATGCACATTCCCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGctt                                                                             <  2:227291/143‑1 (MQ=255)
                                                                  ggAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTaa                                                                        <  1:509638/143‑1 (MQ=255)
                                                                          tCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCCCCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACCTTCAATAGCGAAGAGAAAAAACGCCCCTCAAGCGCAccc                                                                >  2:687783/1‑141 (MQ=255)
                                                                             aGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGAAAATTACCAGCACCGAAGAAAAACTCTGGACCCCACAGCGTATCCAGTCTCGCGGCGCGACTTTCAATAGCGAAGAGAGAAAACGCTTCTTAAGCGAACTGAcc                                                             >  1:164881/1‑143 (MQ=255)
                                                                                aaaCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCCAGTCTGGTCACGCGACTTTCAATTGCGAAGAGAAAAAACGCTTCCTAAACGAACTGAccgcc                                                          >  2:446123/1‑143 (MQ=255)
                                                                                    cTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGCCCGCCGCTg                                                      >  1:195170/1‑143 (MQ=255)
                                                                                            gaacGATTAGTGCGGTGTTTCCGCCCATTTCCTGCCTCGCAGAAAAACTCTGTAGCTAACGGCGTATCGAGTCTGGTCTCGCGACTCTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCtt                                              <  2:460003/140‑1 (MQ=255)
                                                                                              ccGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGGCCGCCGCTGAAGGTCCTGa                                            >  2:695085/1‑143 (MQ=255)
                                                                                                      tGCCGAGTCTATGCACATTACCCGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGATCGCGCGACTTTCAATAGCGAAGAGAAAACACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTAcc                                    <  1:242480/143‑1 (MQ=255)
                                                                                                       gCCGAGTAGAGGCACATTACCAGCACCGAAGAGAGCCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGGGAAAAAGCGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCt                                   <  1:204259/143‑1 (MQ=255)
                                                                                                          gAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACCGACCGCCGCTGAAGGTCATGAACGTTACCTCgg                                >  2:159163/1‑143 (MQ=255)
                                                                                                           ggtgtaggCACATGACCGGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCGGGTCGCGCGACTTTCAATAGCGAAGAGAAAACGCGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGgc                               <  2:180878/136‑1 (MQ=255)
                                                                                                            gTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGgcg                              <  1:476333/143‑1 (MQ=255)
                                                                                                                 tGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCaaaa                         >  1:235124/1‑143 (MQ=255)
                                                                                                                  gCACATTACCAGCACCGAAGAAAAAATCTGGATCCAACAGCGTATCGAGGCCGGTCGCGCGACGGTCAATAGCGAAGAGAAAAAGGGCTGCTTAAGCGACCTGACAGCCGCTGAAGGGCTGGAACGTAACATCGGCGCAGAAt                        >  2:25293/1‑143 (MQ=255)
                                                                                                                        ggccggCATCGAAGAAAAACTCCTGACCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCGTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTg                  <  2:217702/138‑1 (MQ=255)
                                                                                                                           cggCACCGAAGAAGAACTCTGGATCCACCAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgcg               <  1:270925/141‑1 (MQ=255)
                                                                                                                           cAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgcg               >  1:115786/1‑143 (MQ=255)
                                                                                                                            aGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgcgc              <  2:115786/143‑1 (MQ=255)
                                                                                                                                   aagaaAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGc       >  2:249064/1‑143 (MQ=255)
                                                                                                                                        aaaCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAGGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTtctc  >  2:777957/1‑143 (MQ=255)
                                                                                                                                            |                                                                                                                                          
CCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTC  >  NC_000913/759110‑759388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑