| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,560,455:1 | +G | 100% | pseudogene (151/758 nt) | glpR ← | DNA‑binding transcriptional repressor GlpR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,560,455 | 1 | . | G | 91.9% | 104.9 / 2.2 | 37 | pseudogene (151/758 nt) | glpR | DNA‑binding transcriptional repressor GlpR |
| Reads supporting (aligned to +/- strand): ref base . (2/1); new base G (14/20); total (16/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTG‑AGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGT > NC_000913/3560316‑3560586 | gATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCAATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑cgca > 1:579239/1‑140 (MQ=255) aaaCAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGa < 1:277400/143‑1 (MQ=255) gcggcgAGCCATTGGGGATTGGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAgg < 1:126903/143‑1 (MQ=255) gTCGAGCCTTGGGGGATTTGCTCCGCCGCTTTTCGGGCGATGCGCTCTTGTTGTTCGGTCTGGGTGGCCTTGCGAGCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGtt < 2:661179/143‑1 (MQ=255) gTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTAGGTGGCCTTGCGATAGTGACACGGGGTGTTAACCGAGCTGGGAGGCAGCGCCGCGCCGCCCTGATGGG‑G‑CCGGATCAgggt > 1:611442/1‑141 (MQ=255) tCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCGCTGTTTCTTCGGACCGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTTGAAGGCATCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGttt < 1:727818/143‑1 (MQ=255) gAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGACCTGGACGGCAGCGCCGCACCGCCATGCT‑GG‑CGCAGGCTCAGGtttcg > 1:562263/1‑141 (MQ=255) gCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCt > 1:153565/1‑143 (MQ=255) ttGGGGATTTGCTCCGCTACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGc < 2:389537/143‑1 (MQ=255) gggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAg > 1:616321/1‑143 (MQ=255) gggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCACGTTTTTCCACGCCAg > 1:277234/1‑143 (MQ=255) cGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCCCCGCCATGAT‑GG‑CGCCGGATCAGGTTTTGCTCCGCCAGCTCAtcgcggg > 2:204289/1‑137 (MQ=255) tGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGGGTCGGCCCCCCAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTG‑AGGTCGCGGCGa < 1:593739/143‑1 (MQ=255) cGGGCGATGCGCTCTTTTGCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTG‑AGGTCGCGGCGAAt < 1:755567/143‑1 (MQ=255) agggCGAGGCGCGCTTTCGCGTCGGGCGCGGTGGCCGTGCGATCGTGCCACGGCGCGTTAACCGAACGGGAAGCCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTG‑AGGTCGCGGCGAAt < 1:66891/142‑1 (MQ=255) tggCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGTAGCGCCTCACCGCCATGAT‑GG‑CGCAGGATCAGCTTTTGCTCCGCCAGCTCATTG‑AGGTCGCGGCGAATa < 1:584171/142‑1 (MQ=255) ggCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTG‑AGGTCGCGGCGAATAg > 1:655797/1‑143 (MQ=255) cGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGGTTTGCTCCGGCAGCCCATTG‑AGGGCGCGGCGAATAGTc > 2:234545/1‑143 (MQ=255) cgcTCTTTTTCTTCGGTCGGGGTGGCCTTGCGATCGTGCCACGGCGCGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTG‑AGGTCGCGGCGAATAGTCTGCgg < 1:74033/143‑1 (MQ=255) tctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTG‑AGGGGGCGGCGAATAGGCTGCGGGCt > 1:179044/1‑143 (MQ=255) gctgggtctccGGACGGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTG‑AGGTCGCGGCGAATAGTCTGCGGGCt < 1:460986/137‑1 (MQ=255) ttttCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTG‑AGGTCGCGGCGAATAGTCTGCGGGCTGAc > 1:521339/1‑143 (MQ=255) cGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTG‑AGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGaaa > 2:500755/1‑143 (MQ=255) tCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGTG‑CAGGACCACGTAATGACGTGGCA‑CTATTTGTAGGTCGCGGCGAATAGTCTGCGGGCGGAAGGGGAAATGc > 2:171374/1‑143 (MQ=255) gCCTGGCGAGCGTGCCCCGGGGGGTTAGCCGAACTGGAAGGCAGCGCCGCCCCGCCATGATGGG‑G‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTG‑AGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAg < 2:324458/143‑1 (MQ=255) ggacGTGCGGCGGCGTGTTAACCGAACGGGGAGGGCGCTCCGCACACACATGCC‑CG‑CGCACGGGCAGGTTTTGCTCCGCCAGCTCATTG‑AGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg < 1:184593/140‑1 (MQ=255) gcgggCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTGTTGCTCCGCCAGCTCATTG‑AGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGt < 2:389487/139‑1 (MQ=255) cGTGCCAGGGCGTTTTAACCGAACTGAAAGGCAGCGCCGTGCGGCCATGGT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTG‑AGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa < 2:74061/143‑1 (MQ=255) ccACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTG‑AGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGa > 2:713239/1‑143 (MQ=255) gtgtCAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTG‑AGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAcc < 1:277864/143‑1 (MQ=255) agccGAACTGGAGGGCAGCGCCGCGCCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTG‑AGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgct < 2:495152/141‑1 (MQ=255) aaCCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCCCATTG‑AGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCCGCGCGTCGGTACTGACATAACCctgcc > 2:387295/1‑142 (MQ=255) ccGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTG‑AGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGt < 2:616321/143‑1 (MQ=255) gAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTG‑AGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAGATGCGCTACCAGCCCTTCGGCACTGACATAACCCTGGTGttt > 1:335960/1‑143 (MQ=255) gcTGGGAGGCGGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTG‑AGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTaa < 2:626087/142‑1 (MQ=255) cAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTG‑AGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGa < 1:279787/143‑1 (MQ=255) cACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTG‑AGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATCCTCTACCAGCTCTTAGGTACTGACATAAACCTGCTGTTTAACCAGTCCGATAATACCg > 2:280154/1‑143 (MQ=255) gCCATGAT‑GG‑CGCAGGATCAGGTAATGCTTGGCCGGCTCATTG‑AGGTCGCGGCGAAGAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtgt < 1:727561/143‑1 (MQ=255) | GATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTG‑AGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGT > NC_000913/3560316‑3560586 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |