| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,637,645:1 | +T | 100% | coding (4/1500 nt) | pitA → | metal phosphate:H(+) symporter PitA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,637,645 | 1 | . | T | 99.5% | 103.4 / ‑7.0 | 37 | L2L (CTA→TTA) | pitA | metal phosphate:H(+) symporter PitA |
| Reads supporting (aligned to +/- strand): ref base . (0/1); major base T (18/17); minor base A (1/0); total (19/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGC‑T‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCC > NC_000913/3637511‑3637777 | tacgATAATGATTTTTTGAGTGGACACCGTACCGGGGGCAAAGCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACAttt < 1:59482/140‑1 (MQ=255) gATAATTATTTTTTGAGTGAAATCCATACAGGGGGCGAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACAtttgtt > 1:528799/1‑143 (MQ=255) gTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTg < 1:538253/143‑1 (MQ=255) gTGAAATCCATACAGGCGGCAAGTCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGTGTATCGCCCTCTGCTT‑ACATTTGTTTGCTGGCCTGGACTTg < 2:714539/143‑1 (MQ=255) aTACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCggg < 1:493047/143‑1 (MQ=255) aCAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCt < 1:79305/143‑1 (MQ=255) cAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTg > 1:538025/1‑143 (MQ=255) ggggCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCCTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATAGCGGGCCGtca > 2:409846/1‑141 (MQ=255) gCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATTCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTCGTTTGCTGGCCTGGATTTGCATACCGGGCTGttatta > 1:678045/1‑143 (MQ=255) gCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGttatta < 1:300392/143‑1 (MQ=255) aaaTCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTCTGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATATCGCCCTGTTactatt > 1:208654/1‑143 (MQ=255) tataTTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCttt > 1:629109/1‑143 (MQ=255) tttCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTACTTGCACTGGCttttctt > 2:313921/1‑140 (MQ=255) tttCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCACGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCTTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTtgtg < 1:323832/143‑1 (MQ=255) tttCACTTTGCCCGCGCCGCGAAAGGCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGACCTAAGCAA‑ACATTTGTTTGCTGGCCTGGATTTGCATCCCGGGCTGGTCTTATTGCTTGCACTGGCTTTtgtg > 1:812027/1‑143 (MQ=255) tCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCt > 2:241822/1‑143 (MQ=255) tCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCt < 1:317593/143‑1 (MQ=255) cACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTg < 1:623095/143‑1 (MQ=255) cTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCGCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTGGCTGGCCTGGATTTGCATACGGGGCTGTTATTATTGCTTGCCCTGGCTTTTGTGCTggt > 1:669261/1‑141 (MQ=255) ttGCCCGCGCCGACAAAGTCACTCTTAATGCGCCGCGTTCACGTCCTCAAAATGGCGTAACGTCCTATTCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCt < 1:145337/143‑1 (MQ=255) ccgcgccgcgAAAGTCACTGATAATGCGCCGCGTTCCTGTCCTAAAAATGGCGTAACGTACTATGCTT‑GCATTTGTTTGCTGGCCTGGATTTGCATACCGGGATAGTATTATTGCTTGGACTTGCTTTTGTCCTTTTCTACGa > 2:438653/1‑143 (MQ=255) ccgcgccgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTCCGa > 1:683538/1‑143 (MQ=255) ccgcgccgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGGTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGa > 1:613529/1‑143 (MQ=255) cgcgccgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGaa < 1:601150/143‑1 (MQ=255) gcgccgcgAAAGTCACTGATAATGAGAAGCGTTACGGTGCTCAAAATGGCGTAACGGCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACggcg > 1:346263/1‑140 (MQ=255) cgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCAt < 1:703569/143‑1 (MQ=255) cgcgAAAGCCCCTGATAATGCGCCGCGTGCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCAt < 1:613916/143‑1 (MQ=255) tCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGCTTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGACGCCATCAATgggt > 1:44571/1‑141 (MQ=255) gATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCGTCAATGGTTTCCAt > 1:314615/1‑143 (MQ=255) tAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGa < 1:103696/143‑1 (MQ=255) aTGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTATACGAAGACATCAATTGTTTCCATGACACAGCCAACGCCGt > 1:551146/1‑143 (MQ=255) tCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGc > 1:253625/1‑143 (MQ=255) cgcAAACTGGCGTAACGTCCTATGCTT‑GCATTTGTTTGCTGGCCTGTATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCaa < 2:369045/141‑1 (MQ=255) aaaaTGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCg < 1:460134/143‑1 (MQ=255) aaaaTGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCg > 2:87401/1‑143 (MQ=255) gTAGCGTCCTATGC‑TGACATTTGTTTGCTGGCCTGGCTTTGCGTACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCtat < 2:394805/143‑1 (MQ=255) cGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATAccc < 2:310059/143‑1 (MQ=255) | TAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGC‑T‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCC > NC_000913/3637511‑3637777 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |