| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 887,393 | T→A | 10.6% | intergenic (‑54/‑30) | ybjJ ← / → rcdA | inner membrane protein YbjJ/DNA‑binding transcriptional regulator RcdA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 887,393 | 0 | T | A | 10.6% | 98.6 / 3.3 | 45 | intergenic (‑54/‑30) | ybjJ/rcdA | inner membrane protein YbjJ/DNA‑binding transcriptional regulator RcdA |
| Reads supporting (aligned to +/- strand): ref base T (24/13); new base A (3/2); total (27/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.81e-01 | |||||||||||
CATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTG > NC_000913/887256‑887534 | cATTAACAGGCCTGGCAAAAAGAAGAACAAAAACAGGGCCCGGCTTTTGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTac > 1:777588/1‑143 (MQ=255) aTTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTaca > 2:392211/1‑143 (MQ=255) ttAACAGGCCGGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTacac < 2:445490/143‑1 (MQ=255) aaCAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGCAGACAACAATAGCAAGGTTGTGTACAAATGAACACaa > 2:339724/1‑143 (MQ=255) aaCACGACTGGAAAAAAGAAGAACATAAACAGCGCCCACGTTCGGAGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACAAATGTACACaa < 1:464642/143‑1 (MQ=255) cgtgCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATGACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACAAATGTACACaacc < 1:192564/140‑3 (MQ=255) gCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATACGTTCACTATTGCggagag > 1:723858/1‑143 (MQ=255) aaaaaGCAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCagagagag < 2:312852/143‑1 (MQ=255) aaaaGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATAACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACGCAATTGCAGAGAGAgg > 1:205725/1‑143 (MQ=255) gaagcaCACAAACAGCGCGCCGGGTCGGCCTGTCAACGCATTACGAGAAGAATTGACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTGTCTCGGGTCACTCCACTTCCACACAATTGCAGAGAGAGGAAAt < 2:669417/143‑1 (MQ=255) aagaaCATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGCGAGCGGAGATg > 1:670085/1‑143 (MQ=255) aaCATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTGCACAATTGCAGAGAGAGGAAATGaaa > 1:159521/1‑141 (MQ=255) cATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGCGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTAAACAATTGCAGAGAGAGGAAATGACATg > 1:664974/1‑143 (MQ=255) cATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTGGTACACAATTTCATAGATATGAAATGACATg > 1:563134/1‑143 (MQ=255) aGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCTCGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTcgcg < 1:686599/143‑1 (MQ=255) ccAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGCGCGGAACTGACATGCGTCGCGATAAc > 2:586498/1‑143 (MQ=255) ttCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCACAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCGAACGACCCGCAACGGc > 2:236164/1‑143 (MQ=255) ttCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGc > 2:388440/1‑143 (MQ=255) tcaaaccATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCg < 1:444681/137‑1 (MQ=255) aTGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCCGAGAGGCGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCgaga > 2:407264/1‑143 (MQ=255) cATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGCAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGaaaa > 2:160592/1‑143 (MQ=255) aTTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACGCAAGTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCGGCAACGGCGAGaaaaa > 2:612229/1‑143 (MQ=255) aTTACGTAAAGAATTTACGGTCATAGATCACGTAAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGaaaaa < 1:16857/143‑1 (MQ=255) gTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTAt > 2:646769/1‑143 (MQ=255) aagagTTTGCGGTCATAGAGCACGTCAGAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCa < 2:191590/143‑1 (MQ=255) aagaaTTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCa > 2:502595/1‑143 (MQ=255) ggTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTg > 2:646156/1‑143 (MQ=255) ggtCACGTCAAAATACGTAGCGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCgg < 2:246071/141‑1 (MQ=255) gATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATGTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCgg < 1:343711/143‑1 (MQ=255) gTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGACCACGCTGGAGGCGGTGAAAc > 2:177680/1‑143 (MQ=255) aaaTAAGAAGGGGGAAGACAACAGTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTa < 1:707283/143‑1 (MQ=255) aaaTAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTa < 1:365281/143‑1 (MQ=255) aaaTAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTa > 1:616355/1‑143 (MQ=255) aTAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACg > 2:205015/1‑143 (MQ=255) aagaGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCCACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATa > 2:9238/1‑143 (MQ=255) aacagGGAAGACAACATTACCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATa < 1:367745/140‑1 (MQ=255) acggAAGAGAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACCATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACAt < 1:711440/141‑1 (MQ=255) tggAAGACAACATTAGCAAGGTTGTGTACGTGTTTACACAATTGCAGAGATAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATg < 1:111140/142‑1 (MQ=255) gggAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATg > 2:639135/1‑143 (MQ=255) gACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGtt > 1:626454/1‑143 (MQ=255) gACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACCATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGACGCGGTGAAACTCTCCGGAATACATGCtcct > 2:369338/1‑140 (MQ=255) gaTTGGCAAGGTTGTGTACATGTGTACGCGATTGGAGGGACAGGAAATGACATGCGTCGCGCTAACGATCCGCAGCGGCGAGAAAGAATTATCCAGGCCACGCTGTAGGCGGTGAAACTTTACGTAATACATGCTGTTACGcc < 2:6041/142‑2 (MQ=255) ttAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAcc < 1:385218/143‑1 (MQ=255) aGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTGACCCAGCGCaaaa > 2:460236/1‑143 (MQ=255) aattGTGTACAAATGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAAt > 1:275901/3‑143 (MQ=255) tttGTACACAATTGCAGAGAGAGGAAATGACATGTGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTAGGAACCGCAAAATTGATAACATTg > 2:16969/1‑143 (MQ=255) | CATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCACCGCAAAATTGCTACCCTTG > NC_000913/887256‑887534 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |