| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,018,986 | A→T | 12.5% | intergenic (+49/+27) | matP → / ← ompA | macrodomain Ter protein/outer membrane porin A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,018,986 | 0 | A | T | 12.5% | 77.5 / 3.7 | 34 | intergenic (+49/+27) | matP/ompA | macrodomain Ter protein/outer membrane porin A |
| Reads supporting (aligned to +/- strand): ref base A (17/11); new base T (2/2); total (20/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01 | |||||||||||
CTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATT‑‑GCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCC‑GCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAG > NC_000913/1018848‑1019124 | cTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATG‑‑GCTGGGTAAGGAATAACCGACGAAAGAAACAACCAATTACTACAGGCAAAAAAAACGCC‑GCAGCAGCgg > 2:790894/1‑143 (MQ=255) tGAGTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATT‑‑GCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCC‑GCAGCAGCggg < 1:616037/143‑1 (MQ=255) ttGAAGAGACGGAACACAAAGAGAAATACGCGAATAAAATGTCATCTCTGAAGCAGGATCTGCAGGCATT‑‑TCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCC‑GCAGCAGCGGGGtt < 1:282072/143‑1 (MQ=255) aaGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATT‑‑GCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCC‑GCTGCTGCGGGGttttt < 1:637492/143‑1 (MQ=255) aGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTCACACGGGGATTTCATTCACTCAACGTCTTCCGGAGT‑‑CTGACGAAAGTCAGTTCCATTTACTAAAGGCAAAAAAAACCCC‑GCAGCAGCGGGGTTTTTc > 1:597701/1‑143 (MQ=255) ggAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATT‑‑GCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAGAAAAACCCC‑GCTGCTGCGGGGttttttttgcct < 1:290204/143‑8 (MQ=39) aaCAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATT‑‑GCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCC‑GCAGCAGCGGGGATTTTCTACCAGACga > 2:418153/1‑143 (MQ=255) aaTCCGCGAATAAAAGGTCTTCTCTGAAGCAGGATCTGCAGGCATT‑‑GCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCC‑GCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGc < 2:721793/143‑1 (MQ=255) aTAAAATGTCCTCTCGGAAGCAGGAGCTGCAGGCATT‑‑GCTGGGTAAGGAATAACTGAGGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCC‑GCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTg < 2:162877/143‑1 (MQ=255) aaTGTCTTCTCTGAAGCAGGATCTGCAGGCATT‑‑GCTGGGTAAGGAATAACTGACGAACGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCC‑GCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGGGtt > 1:215084/1‑143 (MQ=255) cttctCTGAAGCAGGATCTGCAGGCATT‑‑GCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCC‑GCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAAc > 1:706110/1‑143 (MQ=255) tctGAAGCAGGATCTGCAGGCATT‑‑GCTGGGTAAGGCCTCACTGACGAAAGTCAGTACAATTTACTCCAGGCACAAAAAACCCC‑GCAGCCGCGGGGCACTTCTACCCGGCGAGAACCCAAGCCTCCGGCCGACGTACAACGTCt > 2:50252/1‑143 (MQ=255) tGAAGCAGGATCTGCAGGCATT‑‑GCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCC‑GCAGCAGCGGGGTTTTTCTACCAGACGCGAACTTAAGCCTGCGGCTGAGTTCCAACGTCttt > 2:386466/1‑143 (MQ=255) cTGCAGGCATT‑‑GCGTGGGAAGGAATAACTGAGCAAAGTCAGTCCAATTTACTAAAGGCAAAAAAAACCCC‑GCAGCGGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTaa < 2:350818/143‑1 (MQ=255) cATT‑‑GCGCGCTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTACAGGCAAAAAAAACCCC‑GCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGAt < 1:238040/143‑1 (MQ=255) tt‑‑GCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCC‑GCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGAtct > 2:103256/1‑143 (MQ=255) ggTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCC‑GCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTAcgc < 1:775932/143‑1 (MQ=255) tAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCC‑GCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATAcc < 1:393431/121‑1 (MQ=255) tAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCC‑GCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATAcc > 2:393431/1‑121 (MQ=255) aaGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCC‑GCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGcgac < 2:764524/143‑1 (MQ=255) gAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCAGCAGCAGCGGGGTGTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCGTTGATACATTTAAATTCGATCGCGAGGcgacac > 2:632062/1‑141 (MQ=255) aCGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCC‑GCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGCCTTGGATACCTTAAACAGCGTTCCCTACGCGACGACCCGGAGCCa > 2:390806/1‑143 (MQ=255) cGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCC‑GCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAg < 1:153797/143‑1 (MQ=255) ttCAATTTACTAAAGGCAAAAAAAACCCC‑GCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTGGAta > 2:65954/1‑142 (MQ=255) ctCAATTTACTAAAGGCAAAAAAAACCCC‑GCAGCAGCGGGGTTTTTATACCAGACGAGAAATTAAGCCTGCGGCGGAGTTAGAACGTCGTTGATACCGGTAACTTCGATCTCTACGCGCGGAGCGGGAGCCGGGCAGTCGagc > 1:324780/2‑141 (MQ=255) aaaaaaaaCCCC‑GCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGttt > 2:650931/1‑143 (MQ=255) aaaaaaaCCCC‑GCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACCTCGATCTCTACGCGCCGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTc > 2:21155/1‑143 (MQ=255) aaaaaCCCC‑GCTGCTGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCAc > 1:755783/1‑143 (MQ=255) gtcccc‑GCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTGTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGtt < 2:748924/141‑1 (MQ=255) aaCCCC‑GCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGTAGCCAGGCAGTCGATCAGTGCAGCACGCGGTCTCACGtt > 2:636730/1‑143 (MQ=255) aCCCC‑GCTGCTGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTg > 1:193544/1‑143 (MQ=255) cc‑GCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGCTCAGTGCAGCACGCGGTTTCACGTTGTca > 2:389757/1‑143 (MQ=255) cc‑GCAGCAGCGGGGCTGTGCTGCCGGACGGTGACTTAAGCGCGCGGTTGAGTTACAACGTCTTTGATACCTTTAGCTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTca < 2:592995/143‑1 (MQ=255) c‑GCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTATTTCACGTTGTcac > 1:459807/1‑143 (MQ=255) cagcagCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAg < 1:714898/143‑1 (MQ=255) | CTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATT‑‑GCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCC‑GCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAG > NC_000913/1018848‑1019124 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |