Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,196,277	C→T	38.2%	N385N (AAC→AAT)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,277	0	C	T	38.2%	21.0 / 16.9	24	N385N (AAC→AAT)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (10/4);  new base T (5/4);  total (16/8)
Fisher's exact test for biased strand distribution p-value = 6.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTC‑AGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGT  >  NC_000913/1196135‑1196407                                                                                                                                               |                                                                                                                                    cccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGACATGATGCTGCGCCATATGGGTTGGCCTGACGCGGCTGCCCTCATTGTTAACGGTCTGGCGGGCGCAATCCAc                                                                                                                                     >  1:337497/1‑143 (MQ=25)          cTGCGCCGTAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTACTATTCTCTCCGCTGAGATGACGCTGCGCCCCATGGGCTGGACCGAACCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAAcc                                                                                                                            <  2:348842/143‑1 (MQ=38)             cgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTa                                                                                                                         >  1:568722/1‑143 (MQ=255)                    atatGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAAAGCGAAAACCGTAACCTATg                                                                                                                  >  2:308202/1‑143 (MQ=255)                       tGCCGGTCAGTACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACt                                                                                                               <  1:790896/143‑1 (MQ=14)                             tCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGaac                                                                                                         <  1:663947/143‑3 (MQ=14)                             tCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTAGCACTTCGaac                                                                                                         >  2:342161/1‑141 (MQ=14)                              cAGGACAAAGTCAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTCAAGCGGCTGACCTGCTTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTAGGCCTTCGAGCg                                                                                                        >  1:81760/1‑143 (MQ=14)                                 gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGCACGTCt                                                                                                     >  1:688299/1‑143 (MQ=14)                                 gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTACCCTCAGACTTCGACCGTCt                                                                                                     >  1:723280/1‑143 (MQ=255)                                   cAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGATCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTga                                                                                                   >  1:121115/1‑143 (MQ=11)                                   cAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCGGACCTGAGTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTga                                                                                                   <  2:153007/143‑1 (MQ=11)                                    aaaGTAAAGCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTTATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTgat                                                                                                  <  1:149786/143‑1 (MQ=11)                                       gTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTgatgaa                                                                                               >  2:103485/1‑141 (MQ=11)                                                        tattCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGa                                                                              >  1:663179/1‑143 (MQ=255)                                                                ccGCTGAGATGATGCTGCGCCACATGGGTTGGACCGACGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGAGGGCGCTAAACTGCTGAAATGTTcc                                                                      >  2:798863/1‑142 (MQ=255)                                                                ccGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTC‑a                                                                     <  2:250348/143‑1 (MQ=255)                                                                         ggagggtgcGCCACATGGGTGGGACCGCAGCGGCTGACTTAATTGTTCAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTC‑AGAGTTTGGt                                                            <  1:462263/137‑1 (MQ=18)                                                                                gcgcCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGCGGCTAAAACGCTGCAATCTTC‑CGAGGTTGGTGGCGGGa                                                     >  2:770294/1‑143 (MQ=18)                                                                                  gcCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTC‑AGAGTTTGGTGACGCGatc                                                   >  2:263974/1‑143 (MQ=255)                                                                                              ggACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTC‑AGAGTTTGGTGACGCGAGCATCGAAAACATg                                       >  1:475116/1‑143 (MQ=255)                                                                                                        ggCTGACCTAATTGTTAAAGGTATGGACGGCGCAACCAACGCGAAAACCGCAACCTATGACTTCGACAGCCTGATGGATGGCGCTAGTCTTCTCTAA‑GTCCAAGAGTCTGGTCATGCAATCATCGAAAACATGTAATGCCGTa                             <  2:329258/143‑1 (MQ=9)                                                                                                                 aaTTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTC‑AGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGCTTGTTaa                    >  2:263986/1‑143 (MQ=255)                                                                                                                             tATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTC‑AGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTGTGTTAAATTTATTAACgg        >  2:237319/1‑143 (MQ=255)                                                                                                                                   aGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTC‑AGAGTTTGGTGACGCGCTCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGt  >  2:526369/1‑143 (MQ=255)                                                                                                                                               |                                                                                                                                    CCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTC‑AGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGT  >  NC_000913/1196135‑1196407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑