| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,196,304 | G→A | 24.3% | E394E (GAG→GAA) | icd → | isocitrate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,196,304 | 0 | G | A | 24.3% | 29.9 / 4.2 | 23 | E394E (GAG→GAA) | icd | isocitrate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (11/5); new base A (3/2); total (15/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
CAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTC‑AGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTA > NC_000913/1196164‑1196445 | cAGGACAAAGTCAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTCAAGCGGCTGACCTGCTTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTAGGCCTTCGAGCg > 1:81760/1‑143 (MQ=14) gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGCACGTCt > 1:688299/1‑143 (MQ=14) gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTACCCTCAGACTTCGACCGTCt > 1:723280/1‑143 (MQ=255) cAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGATCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTga > 1:121115/1‑143 (MQ=11) cAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCGGACCTGAGTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTga < 2:153007/143‑1 (MQ=11) aaaGTAAAGCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTTATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTgat < 1:149786/143‑1 (MQ=11) gTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTgatgaa > 2:103485/1‑141 (MQ=11) tattCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGa > 1:663179/1‑143 (MQ=255) ccGCTGAGATGATGCTGCGCCACATGGGTTGGACCGACGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGAGGGCGCTAAACTGCTGAAATGTTcc > 2:798863/1‑142 (MQ=255) ccGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTC‑a < 2:250348/143‑1 (MQ=255) ggagggtgcGCCACATGGGTGGGACCGCAGCGGCTGACTTAATTGTTCAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTC‑AGAGTTTGGt < 1:462263/137‑1 (MQ=18) gcgcCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGCGGCTAAAACGCTGCAATCTTC‑CGAGGTTGGTGGCGGGa > 2:770294/1‑143 (MQ=18) gcCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTC‑AGAGTTTGGTGACGCGatc > 2:263974/1‑143 (MQ=255) ggACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTC‑AGAGTTTGGTGACGCGAGCATCGAAAACATg > 1:475116/1‑143 (MQ=255) ggCTGACCTAATTGTTAAAGGTATGGACGGCGCAACCAACGCGAAAACCGCAACCTATGACTTCGACAGCCTGATGGATGGCGCTAGTCTTCTCTAA‑GTCCAAGAGTCTGGTCATGCAATCATCGAAAACATGTAATGCCGTa < 2:329258/143‑1 (MQ=9) aaTTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTC‑AGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGCTTGTTaa > 2:263986/1‑143 (MQ=255) tATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTC‑AGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTGTGTTAAATTTATTAACgg > 2:237319/1‑143 (MQ=255) aGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTC‑AGAGTTTGGTGACGCGCTCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGt > 2:526369/1‑143 (MQ=255) aCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTC‑AGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGtttttt < 1:380823/143‑1 (MQ=255) ccGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTC‑AGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTg < 1:263974/143‑1 (MQ=255) tAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTC‑AGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTa < 2:568722/143‑1 (MQ=255) tAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTC‑AGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGCGAGCGTAACGCTTCCGTTGTTTTTTGttt > 1:566203/1‑142 (MQ=255) tGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTC‑AGAGTTTGGTGACGCGATCATCGAACACGTGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGATCCGGTTGTTTTTTGTTAGgctggt > 1:322109/1‑141 (MQ=255) ggCGTCTGATGGATGGCGCTAAACTGCTGAACTGTCA‑AGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTa < 1:770294/142‑1 (MQ=255) | CAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTC‑AGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTA > NC_000913/1196164‑1196445 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |