Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,928,799 A→C 9.0% L14R (CTT→CGT)  ptrB ← oligopeptidase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,928,7990AC 9.0% 105.3 / 4.7 46L14R (CTT→CGT) ptrBoligopeptidase B
Reads supporting (aligned to +/- strand):  ref base A (20/21);  new base C (2/2);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.65e-02

GATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGAC  >  NC_000913/1928657‑1928932
                                                                                                                                              |                                                                                                                                     
gATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGaa                                                                                                                                       >  1:23891/1‑143 (MQ=255)
gATGCGATCCTGCAAGGCTGGTGGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGaa                                                                                                                                       <  1:260849/143‑1 (MQ=255)
   gCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCg                                                                                                                                    >  2:719376/1‑143 (MQ=255)
      aTCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCa                                                                                                                                 >  1:791581/1‑143 (MQ=255)
        ccTTCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTACCTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATg                                                                                                                               <  2:7148/143‑1 (MQ=255)
           gccaGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGACGCTTCATGGCg                                                                                                                            <  2:262412/140‑1 (MQ=255)
            cAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGACGCTTCATGGCga                                                                                                                           <  2:384845/143‑2 (MQ=255)
               ggCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTggg                                                                                                                        <  1:146660/143‑1 (MQ=255)
                     ttgtGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATgc                                                                                                                  >  2:295839/1‑143 (MQ=255)
                        tgAGGCCATCGCCCGCTGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATgcggg                                                                                                               <  1:334339/143‑1 (MQ=255)
                              cATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCCTGGCGGGGGGAGTGCGGGGGGCtg                                                                                                         >  2:743902/1‑142 (MQ=255)
                                  aCCCGATGTCCGTAACTATTTTCTTGCTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTgg                                                                                                     <  1:563699/143‑1 (MQ=255)
                                    ccGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTa                                                                                                   >  1:546288/1‑143 (MQ=255)
                                                  tATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttc                                                                                     >  1:94268/1‑143 (MQ=255)
                                                     tttCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttcttt                                                                                  >  2:340597/1‑143 (MQ=255)
                                                         ttgttgCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGATGCGGCATCGCGTGGGGAATGCGGGCGGCTCTTGGTAGCATGTTATTGTTCTTTTTgg                                                                              >  2:94228/1‑143 (MQ=255)
                                                                   tcgtgcAGGACTTCTCGCGGAGAACGCGTAGCGTCCCGCAGCCAGTAGTAATTATCGATGTGCGTATGGCGATGAAGCGTCATTGCTTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATc                                                                    <  2:55463/138‑1 (MQ=255)
                                                                      tCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCGTTTGGTAGCATGTTATTGTTCTTTCTGGTTGACACATCGta                                                                 >  1:530728/1‑143 (MQ=255)
                                                                        cAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTGATAGTTCTTTCTGGTTGAAACATCTtata                                                               >  2:677495/1‑143 (MQ=255)
                                                                           gACTTGTTGCTGTGAACGCGTCTCGCCCCGTAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGGTATTGTTCTTTCTGGTTGAAACATCTTATAAgg                                                            <  2:558750/143‑1 (MQ=255)
                                                                             cTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGt                                                          >  1:744818/1‑143 (MQ=255)
                                                                             cTTCGGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATTGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGt                                                          <  1:682730/143‑1 (MQ=255)
                                                                                  ggCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCaa                                                     <  1:719376/143‑1 (MQ=255)
                                                                                  ggCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCaa                                                     >  1:311157/1‑143 (MQ=255)
                                                                                   gCTGAGAACGCGTATCGTCCCGCAGCCAGTAGAAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCATTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCGTAAAAGGGTGGCaaa                                                    >  2:31271/1‑143 (MQ=255)
                                                                                    cggagaGCGCGTTTCGTCCCGCGGCCAGTAGGAATTAGCGAAGAATGGATGGGGGCGAAGCATCATTGTGTTTGGTATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCaaaa                                                   <  1:151653/141‑1 (MQ=255)
                                                                                    cTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCaaaa                                                   <  1:566251/143‑1 (MQ=255)
                                                                                        gaACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCa                                               >  1:787144/1‑143 (MQ=255)
                                                                                         aaCGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCCTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCAc                                              >  2:259468/1‑143 (MQ=255)
                                                                                                    tCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCaa                                   <  1:738085/143‑1 (MQ=255)
                                                                                                        gCAGCCAGTAGCAATTATCGATGCGCGTAGCGCCATGAAGCGTCACGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGa                               <  1:322955/143‑1 (MQ=255)
                                                                                                             cAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAg                          <  1:186866/143‑1 (MQ=255)
                                                                                                                tagtaATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAgggg                       >  1:256430/1‑143 (MQ=255)
                                                                                                                 agtaATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGa                      <  1:340597/143‑1 (MQ=255)
                                                                                                                 agtaATTATCGAGGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTGTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAGGCGAAACAGGGGa                      <  1:748440/143‑1 (MQ=255)
                                                                                                                     aatATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGAGGAATGCGGGCGGCTATTGGAAGCATGTTATTGTTCTTTCTGGTTGAAACATCTGATAAGGGTGGAAAAACTAACCGGCGAGACAAGAGAAACAGGGGAgaac                  >  2:390115/3‑140 (MQ=255)
                                                                                                                      ttATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGAGAAACAGGGGAGTCac                 >  2:17540/1‑142 (MQ=255)
                                                                                                                        aTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAACCGGGGGAGTCAtcg               >  1:722685/1‑141 (MQ=255)
                                                                                                                        aTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTg               <  1:448635/143‑1 (MQ=255)
                                                                                                                            aTGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTa           >  2:182734/1‑143 (MQ=255)
                                                                                                                            aTGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTa           <  1:295839/143‑1 (MQ=255)
                                                                                                                             tGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAg          <  2:197631/143‑1 (MQ=255)
                                                                                                                              gcgcGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTAGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACA‑GGGAGTCATTGCTTAgat        <  2:64733/143‑1 (MQ=255)
                                                                                                                                 tgTATCGTTATGAAGCGTAATGGCGTGGGGAATGCGGGCGGCTGGTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAgatga      <  1:49144/142‑1 (MQ=255)
                                                                                                                                    cacGCCATGACGCTTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGACACATCTTATAAGGTTGGCAAAACTCACCGGGGATGCACGCGAAACCGGGGAGACATCGCTTAGAcgatga   >  2:417717/3‑143 (MQ=255)
                                                                                                                                    cacGCCATGACGCTTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAACCATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGAtgacga   >  2:323979/3‑143 (MQ=255)
                                                                                                                                     tggcgaggAAGCGTCATGGCGTGGGGAGTGCGGGCGGCTTGTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGAc  <  1:94228/136‑1 (MQ=255)
                                                                                                                                     tCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGCTGAc  >  1:304126/1‑143 (MQ=255)
                                                                                                                                              |                                                                                                                                     
GATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGAC  >  NC_000913/1928657‑1928932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑