Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,928,802	G→T	9.0%	T13K (ACG→AAG)	ptrB ←	oligopeptidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,928,802	0	G	T	9.0%	114.9 / 2.8	45	T13K (ACG→AAG)	ptrB	oligopeptidase B
Reads supporting (aligned to +/- strand):  ref base G (20/20);  new base T (2/2);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.35e-01

GCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGAC  >  NC_000913/1928660‑1928932                                                                                                                                               |                                                                                                                                   gCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCg                                                                                                                                    >  2:719376/1‑143 (MQ=255)    aTCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCa                                                                                                                                 >  1:791581/1‑143 (MQ=255)      ccTTCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTACCTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATg                                                                                                                               <  2:7148/143‑1 (MQ=255)         gccaGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGACGCTTCATGGCg                                                                                                                            <  2:262412/140‑1 (MQ=255)          cAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGACGCTTCATGGCga                                                                                                                           <  2:384845/143‑2 (MQ=255)             ggCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTggg                                                                                                                        <  1:146660/143‑1 (MQ=255)                   ttgtGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATgc                                                                                                                  >  2:295839/1‑143 (MQ=255)                      tgAGGCCATCGCCCGCTGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATgcggg                                                                                                               <  1:334339/143‑1 (MQ=255)                            cATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCCTGGCGGGGGGAGTGCGGGGGGCtg                                                                                                         >  2:743902/1‑142 (MQ=255)                                aCCCGATGTCCGTAACTATTTTCTTGCTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTgg                                                                                                     <  1:563699/143‑1 (MQ=255)                                  ccGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTa                                                                                                   >  1:546288/1‑143 (MQ=255)                                                tATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttc                                                                                     >  1:94268/1‑143 (MQ=255)                                                   tttCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttcttt                                                                                  >  2:340597/1‑143 (MQ=255)                                                       ttgttgCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGATGCGGCATCGCGTGGGGAATGCGGGCGGCTCTTGGTAGCATGTTATTGTTCTTTTTgg                                                                              >  2:94228/1‑143 (MQ=255)                                                                 tcgtgcAGGACTTCTCGCGGAGAACGCGTAGCGTCCCGCAGCCAGTAGTAATTATCGATGTGCGTATGGCGATGAAGCGTCATTGCTTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATc                                                                    <  2:55463/138‑1 (MQ=255)                                                                    tCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCGTTTGGTAGCATGTTATTGTTCTTTCTGGTTGACACATCGta                                                                 >  1:530728/1‑143 (MQ=255)                                                                      cAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTGATAGTTCTTTCTGGTTGAAACATCTtata                                                               >  2:677495/1‑143 (MQ=255)                                                                         gACTTGTTGCTGTGAACGCGTCTCGCCCCGTAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGGTATTGTTCTTTCTGGTTGAAACATCTTATAAgg                                                            <  2:558750/143‑1 (MQ=255)                                                                           cTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGt                                                          >  1:744818/1‑143 (MQ=255)                                                                           cTTCGGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATTGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGt                                                          <  1:682730/143‑1 (MQ=255)                                                                                ggCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCaa                                                     <  1:719376/143‑1 (MQ=255)                                                                                ggCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCaa                                                     >  1:311157/1‑143 (MQ=255)                                                                                 gCTGAGAACGCGTATCGTCCCGCAGCCAGTAGAAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCATTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCGTAAAAGGGTGGCaaa                                                    >  2:31271/1‑143 (MQ=255)                                                                                  cggagaGCGCGTTTCGTCCCGCGGCCAGTAGGAATTAGCGAAGAATGGATGGGGGCGAAGCATCATTGTGTTTGGTATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCaaaa                                                   <  1:151653/141‑1 (MQ=255)                                                                                  cTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCaaaa                                                   <  1:566251/143‑1 (MQ=255)                                                                                      gaACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCa                                               >  1:787144/1‑143 (MQ=255)                                                                                       aaCGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCCTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCAc                                              >  2:259468/1‑143 (MQ=255)                                                                                                  tCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCaa                                   <  1:738085/143‑1 (MQ=255)                                                                                                      gCAGCCAGTAGCAATTATCGATGCGCGTAGCGCCATGAAGCGTCACGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGa                               <  1:322955/143‑1 (MQ=255)                                                                                                           cAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAg                          <  1:186866/143‑1 (MQ=255)                                                                                                              tagtaATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAgggg                       >  1:256430/1‑143 (MQ=255)                                                                                                               agtaATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGa                      <  1:340597/143‑1 (MQ=255)                                                                                                               agtaATTATCGAGGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTGTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAGGCGAAACAGGGGa                      <  1:748440/143‑1 (MQ=255)                                                                                                                   aatATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGAGGAATGCGGGCGGCTATTGGAAGCATGTTATTGTTCTTTCTGGTTGAAACATCTGATAAGGGTGGAAAAACTAACCGGCGAGACAAGAGAAACAGGGGAgaac                  >  2:390115/3‑140 (MQ=255)                                                                                                                    ttATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGAGAAACAGGGGAGTCac                 >  2:17540/1‑142 (MQ=255)                                                                                                                      aTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAACCGGGGGAGTCAtcg               >  1:722685/1‑141 (MQ=255)                                                                                                                      aTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTg               <  1:448635/143‑1 (MQ=255)                                                                                                                          aTGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTa           <  1:295839/143‑1 (MQ=255)                                                                                                                          aTGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTa           >  2:182734/1‑143 (MQ=255)                                                                                                                           tGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAg          <  2:197631/143‑1 (MQ=255)                                                                                                                            gcgcGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTAGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACA‑GGGAGTCATTGCTTAgat        <  2:64733/143‑1 (MQ=255)                                                                                                                               tgTATCGTTATGAAGCGTAATGGCGTGGGGAATGCGGGCGGCTGGTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAgatga      <  1:49144/142‑1 (MQ=255)                                                                                                                                  cacGCCATGACGCTTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGACACATCTTATAAGGTTGGCAAAACTCACCGGGGATGCACGCGAAACCGGGGAGACATCGCTTAGAcgatga   >  2:417717/3‑143 (MQ=255)                                                                                                                                  cacGCCATGACGCTTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAACCATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGAtgacga   >  2:323979/3‑143 (MQ=255)                                                                                                                                   tggcgaggAAGCGTCATGGCGTGGGGAGTGCGGGCGGCTTGTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGAc  <  1:94228/136‑1 (MQ=255)                                                                                                                                   tCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGCTGAc  >  1:304126/1‑143 (MQ=255)                                                                                                                                               |                                                                                                                                   GCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGAC  >  NC_000913/1928660‑1928932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑