| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | 100% | I204S (ATC→AGC) | wcaA ← | putative colanic acid biosynthesis glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 100.0% | 73.9 / ‑6.7 | 28 | I204S (ATC→AGC) | wcaA | putative colanic acid biosynthesis glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (13/14); minor base G (1/0); total (14/14) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTG‑CGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAC > NC_000913/2132647‑2132920 | ggATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGCGGTGATCTGCATTTCACCGTGATTGATCTGCCGGCTc > 1:565561/1‑143 (MQ=255) gaccTGGTAGCGTGTAAACCGCTGACATTTTTTGCTGGCGCGGTCGCATTTGTCTTTGTGCTTGCGCTAAAAGTGTAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGctct < 1:50660/140‑1 (MQ=255) tCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCGTTTCACCGTGATTGATATGCAGGCTCTG‑c > 1:137196/1‑143 (MQ=255) tGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTG‑CGt < 1:112468/143‑1 (MQ=255) ggTAGAGGGTGATCAGCTGATATGTTTTGTTGGCGGGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTG‑CGTc < 2:958/143‑1 (MQ=255) tAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTG‑CGTCGc < 1:591334/143‑1 (MQ=255) aaaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTG‑CATCGCCTCTTCTa > 1:257128/1‑143 (MQ=255) atatTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGAGTGACA‑ACCGGGTGTGTGGGCGCCTCTTCTACTTTcctgg > 1:631280/1‑140 (MQ=255) tatTTGTGGCTGGCGGGGTCGCATTGGTCTTTGTGCTTGCGGTAAAGGTGGAAGGACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTG‑CGTCGCCTCTTCTACTTTCCACgg < 2:224799/143‑1 (MQ=255) ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTG‑CGTCGCCTCTTCTACTTTCCACGGtt > 1:496353/1‑143 (MQ=255) ttGCTGGCGCGGTCGAGTTTGTGTTTTGGCTTGGGGTCAGGACGGAATGTCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTG‑CGTCGCCTCTTCTACTTTCCACGGTTCGcc < 2:574668/143‑1 (MQ=255) ggCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTG‑CGTCGCCTCTTCTACTTTGCACGGGTCGCCgcaac > 2:709386/1‑139 (MQ=255) gagaTTTGTCTTTGTGCTTGGGGTCAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCCTTTCACCGTGATTGATATGCAGGCTCTG‑CGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccacc < 2:341736/140‑1 (MQ=255) tttggtgtttGTGCGTGCGGTCAAAGTGGAAGTACCCGCAGACTTTTTTCGGGGGCGAGGTGATCTGCATTTCACCGTGATTGATAGGCAGGCTCTG‑CGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATc < 2:464091/136‑1 (MQ=255) tgCCTGCGGTAAACCGGCAAGTACCCTGAGCATTTTTTCGGTGACGAGGCGATCTGCATTTCACCGTGATTGATATGCAGGCTCTG‑CGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGAt < 2:228356/143‑1 (MQ=255) gCTTGGGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATAGGCAGGCTCTG‑CGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATg < 2:116755/143‑1 (MQ=255) cTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGACGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTG‑CGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACAACCATCCGCAGGAAGATGt > 1:388606/1‑143 (MQ=255) gTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTG‑CGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg > 1:386101/1‑143 (MQ=255) tGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGTTATGCAGGCTCTG‑CGTCGCGTCTTCTACTTTCTACGGTTCGCCGTACTCCACCACCATCCGCGGGGCGATGTCGTAGTCCTGTGc > 2:430082/1‑143 (MQ=255) aCCCGGAGCATTTTTTCGGTGACGAAGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTG‑CGTCGCCTCTTCGACTTGCCACGGTTCGCCGGCCTCCACCACCAGCCGCAGGAAGCGGTTGTAATCCTGTGCCGCTGTc > 1:549035/1‑143 (MQ=255) gagaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTG‑CGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCTGGTGGATGTCGTAATCCTGTGCCGCTTTCAGTTc < 2:496353/143‑1 (MQ=255) ttttCGGTGACGAGGTGATCTGCATTTCACCGTGTTTGATATGCAGGCTCTG‑CGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCg < 2:636117/143‑1 (MQ=255) gttCTGCATTTCACCTTGATTTGTATGCAGGCTCTG‑CGTCGCCTCTTCTACTTGCCTCGGGTCGCCGTACTCCACCACCAGCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGa < 2:323482/141‑1 (MQ=255) tCTGCATTTCACCGTGATTGATATGCAGGCTCTG‑CGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaaa > 1:624534/1‑143 (MQ=255) gCATTTCACCGTGATTGATATGCAGGCTCTG‑CGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGc > 1:580495/1‑143 (MQ=255) tCACCGTGGTTGATATGCAGGCTCTG‑CGTCGCGTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGACGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGc < 2:282157/143‑1 (MQ=255) attgatATGCAGGCTCTG‑CGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGGAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGACCAgggag > 2:8576/1‑140 (MQ=255) tgatATGCAGGCCCTG‑CGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTGATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAg < 1:355211/143‑1 (MQ=255) atatGCAGGCTCTG‑CGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGACGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCCGGCAAAGAc > 2:372749/1‑143 (MQ=255) | GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTG‑CGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAC > NC_000913/2132647‑2132920 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |