| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,661,969 | C→G | 100% | V55L (GTT→CTT) | iscR ← | DNA‑binding transcriptional dual regulator IscR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,661,969 | 0 | C | G | 100.0% | 106.6 / NA | 36 | V55L (GTT→CTT) | iscR | DNA‑binding transcriptional dual regulator IscR |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (19/17); total (19/17) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTAC‑TTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCA > NC_000913/2661836‑2662105 | tGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTAC‑TTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCAtt < 1:785378/143‑1 (MQ=255) ggCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTTTGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGCTAACCACCGCCTGGTCCACGTACGCTGGACAGCAGACCAtt > 2:785810/1‑143 (MQ=255) ggCAGCCGCCGTTACCCTGACAACGGGTGGCATCTTCAGATTCGTCAACGGCGCTAATTAC‑TTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCAttt < 2:414990/143‑1 (MQ=255) aGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTAC‑TTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTACACCTACGCCGGAACGCAGACCACTTTTa > 2:31938/1‑143 (MQ=255) tttACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTAC‑TTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGAc < 1:146322/143‑1 (MQ=255) ccTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAAGTAC‑TTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGCTCCACGGACGCCGGAAAGCAGACAACTCTTACGCACACgggca > 2:720802/1‑141 (MQ=255) cTGACAACTGGTGGAATCTTCAGTTTCGTCAACGGCGCTAATTAC‑TTCGCCAACGGCGATGCTGCGGGCAGCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGaaa < 1:274562/143‑1 (MQ=255) aCAACGGGTGGCATCTACAGATTCGGCAACGGCGCTAATTAC‑TTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACa < 1:129509/143‑1 (MQ=255) gcgtCTACTGATTCGGCAACGGCGCTAAGTAC‑TTCGCCGACGGCGATGCTGCTGGCATCTTGGCCGAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAg < 2:118341/140‑1 (MQ=255) cagcTACAGAGTCGGCCACGGCGCTAATTAC‑TTCGCATACAGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCAGCGCCTGGTCCACGTACGCTGGAAAGCAGACCTTTTTTACGCAGACGGTAAAACAGTTGTTCCAGa < 2:58214/140‑1 (MQ=255) cATCTACAGATTCGTCAACGGCGCTAATTAC‑TTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCCGGAAAGCATACCCTTTTTACCGAGCCGGGAAAACAGTTGTTcccgc > 2:119216/1‑140 (MQ=255) gTCAACGGCGCTACTTAC‑TTCGCCAACGGCGATGCTGCTGGCATCTTGGCCTAACAGATAACCTCCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGaaa < 2:62168/143‑1 (MQ=255) gTCAACGGCGCTAATTAC‑TTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTAAGCTGGACAGCAGACCATTTTTACGCAGACGGGACCACAGCTGTTCCAGATAAGAAAGGGaaa > 1:408597/1‑143 (MQ=255) tCAACGGCGCTAATTAC‑TTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCAGCGGTTGGACCACCTACGCGGGAAAGCAGACAATTTTTAGGCGGCCGGGAGAGCAGTTGTTCCGGATCGGAAAGGGaaac > 2:97537/1‑142 (MQ=255) cAACGGCGCTAATTAC‑TTCGCCAACGGCGATGCTGCTGGCATCTTTGCATACCAGATAACCACCGCCTGGTCCACGGACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAAtt < 2:487565/143‑1 (MQ=255) tAATTAC‑TTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGt > 1:232595/1‑143 (MQ=255) aTTAC‑TTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGCTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTc < 1:381556/143‑1 (MQ=255) ttCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaaa < 1:439683/143‑1 (MQ=255) ttCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaaa > 1:20212/1‑143 (MQ=255) gCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGCAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACCTTCGGacctat > 2:77081/1‑138 (MQ=255) gCGATTCTGCTTGCATCTTTGCTTAACAGATAACCACCGCCTGGTCCACGTACGCTTGATAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGATAGGGAAATTCCCTGACGTTCGGAAATATCAGCCaa < 2:391730/143‑1 (MQ=255) gctgctGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAACACAGTTGTTCCAGATAAGAAAGGGAAATTCCCCGCCGTTCGGAAAGCTCAGCCACCGGTa > 2:47167/1‑143 (MQ=255) cATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAATATCAGCCAACGGTACCGGGccc > 1:219527/1‑143 (MQ=255) cATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCGGACGTTCGGAAATATCAGCCAACGGTACCGGGccc > 1:259215/1‑143 (MQ=255) cATCTTTGCATAACAGATAACCACCGCCTGGTCCACGTACGATGCAAAGCAGAACAATTTTACGCAGACGGGAAAACAGTTGTTCCAAAAAAGAAAGGGAAATTCCCTGACGTTAGGAAAAATAAGCCAAGGGTAACGGGccc > 2:653620/1‑143 (MQ=255) tCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATGCCCGGACGTTCGGAAATATCAGACAACGGTACCGGGCCCGc > 2:597133/1‑143 (MQ=255) ttGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACCGGAAAACAGTTGTTCCAGATACGAAAGGGAAATTCCCTGACGTTCGGAAATATCAACCAACGGTACTCGGCCCGCTTc > 2:693185/1‑143 (MQ=255) gCCCAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTCGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCag < 1:97537/143‑1 (MQ=255) gcTAACACATCACCACCGCCTGGTCCACGTACGCTGGAGAGCAGACCATTTTTGCGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCaga < 2:370914/142‑1 (MQ=255) tAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGt > 1:275892/1‑143 (MQ=255) aaCAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCACCGGTACCGGGCCCGCTTCAGAGtt < 1:175874/143‑1 (MQ=255) aGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTGCGGGTTGAg > 2:472912/1‑143 (MQ=255) ccGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAAAAAGAGAAATACACTGACGTACGGAAATATCAGCCAACGGTACCGGGCACGCTTCAGAGTTGAGCGCAACGTc > 1:40273/1‑143 (MQ=255) cgtGGTCCAGGTGCGCGGGAAGGCAGACCATGTTTACGCAGACGGGAAAACGGTGGGTCCAGATAAGACAGGGAAGGCCCCTGACGTTCGGAAATATCAGCCAACGGGACCGGGCGCGCTTCAGAGTTGCGCGCAACGTCAAg < 2:18355/141‑1 (MQ=255) tCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTg < 1:563578/143‑1 (MQ=255) tGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCa > 2:466176/1‑143 (MQ=255) | TGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTAC‑TTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCA > NC_000913/2661836‑2662105 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |