| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,761,620 | T→G | 10.7% | K641Q (AAG→CAG) | abpB ← | CP4‑57 prophage; putative helicase YfjK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,761,620 | 0 | T | G | 10.7% | 142.4 / 7.4 | 58 | K641Q (AAG→CAG) | abpB | CP4‑57 prophage; putative helicase YfjK |
| Reads supporting (aligned to +/- strand): ref base T (26/25); new base G (5/2); total (31/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.31e-01 | |||||||||||
TTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTG > NC_000913/2761480‑2761758 | ttCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGCATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTa < 1:341622/143‑1 (MQ=255) ccggCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACGTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACa < 2:368994/140‑1 (MQ=255) aGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGc > 1:14297/1‑143 (MQ=255) aGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGc > 1:366770/1‑143 (MQ=255) cAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCgt < 2:567846/143‑1 (MQ=255) aaGTCTTGCAGCAGGTGTAACGGCGGAATGGGCATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGGAGACGGAAACTTCCGAACGCCAATTTGACATGTGTGAAATCTACTGTGCTGCGTATCCCACGCTTTACAGCgtg < 2:14704/143‑1 (MQ=255) aaGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCgtg < 1:787019/143‑1 (MQ=255) aaGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCgtg < 1:644351/143‑1 (MQ=255) aCCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGGGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGttt > 1:439055/1‑143 (MQ=255) ccAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTa > 1:18962/1‑143 (MQ=255) gtgtAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACAt > 1:561848/1‑143 (MQ=255) tAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACAtcct > 1:584350/1‑143 (MQ=255) aCGGCTGAATGGGAATGCCAATCTCTTCTAAGGCATCGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACAtcctcc < 2:148734/143‑1 (MQ=255) tCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATc < 1:272189/143‑1 (MQ=255) ggAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTAAACCACGCTGTACAGCGTGGTGTTTAACTACATCCTCCATCaa < 2:493746/142‑1 (MQ=255) aaTGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGc > 2:514886/1‑143 (MQ=255) aaTGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATCTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGc > 2:39598/1‑143 (MQ=255) aaTGGGAATGCCAATCTCTTCTAAGGCATTGACAACTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACGTCCTCCATCaacc > 2:404823/1‑141 (MQ=255) gggAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGa < 1:476835/143‑1 (MQ=255) aTGCCTATCCCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCTAACGCCAATTTACGATTTGTGTAATCGACTTTGCTGCGTATACCACGCTGTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCAc < 2:200380/143‑1 (MQ=255) aaTCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtag < 1:191502/143‑1 (MQ=255) aTCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtcgg > 2:667699/1‑143 (MQ=255) ctctTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggta < 1:579240/143‑1 (MQ=255) cttctAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCGCCATCAAGCTGAGCACCTtaggtagg < 2:381524/143‑1 (MQ=255) tctAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGt > 1:151641/1‑143 (MQ=255) tAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTTGTCTTTAACTACATCCTCCATCAAGCTGAGAAACTAAGGAAGAGtat > 2:82318/1‑143 (MQ=255) ggCATTGACACCTGGTGGTAGATGGACACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGc < 1:395317/143‑1 (MQ=255) cATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCg < 1:362414/143‑1 (MQ=255) aTTGACACCTGTTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGa < 2:68997/143‑1 (MQ=255) aTTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTATGTAGGGTATAGCCGa > 1:166811/1‑143 (MQ=255) tGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATCCCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAg > 2:54056/1‑143 (MQ=255) ggtGGATGGAAACTTTCGAACGCCAATTTGACATTTGTGTAATCGACTTTGCTGCGTATACCACGCTTTCCAGCGTGGTGTTTAACTACATCCTCCATCAAGCGGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTAt < 2:341617/143‑1 (MQ=255) tAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATgc > 2:370948/1‑142 (MQ=255) tGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCATTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCAGAAGGTATTGGTCATGAGCtt > 2:646302/1‑143 (MQ=255) aaCGTTCGAACGCTAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCaaa < 1:42569/143‑1 (MQ=255) tttCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATAACACGCTTTACAGCGTGGTGATTAACTACATCCTCCCTCATGCTCAGCACCTTAGAGCGGGTATAGCCGAAGGTCTCGGTCATGAGGTTGaaaata > 2:25308/1‑140 (MQ=255) aaCGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCAttgtt < 1:789245/143‑1 (MQ=255) aCGCCGATTTCACATGTGTGTAATCGACTTTGCTGCGTATACCCCGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCAttgttg < 2:383498/143‑1 (MQ=255) gCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTAAg > 1:410668/1‑143 (MQ=255) cAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAggg > 1:434645/1‑143 (MQ=255) tGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATg < 1:390559/143‑1 (MQ=255) aTGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCCCCATCCAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGaaa < 1:798460/143‑1 (MQ=255) gtgtgtAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTCTTGGTTATGAGCTTCGAATCATTGTTGAGGGCAATGGAAaga > 2:529922/1‑143 (MQ=255) tgtgtAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAagag > 1:413589/1‑143 (MQ=255) aaTCGACGTTGCCGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAACACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGt < 1:792478/143‑1 (MQ=255) tCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGttt > 2:672186/1‑143 (MQ=255) aCTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTGCGGTAGGGTATAGCCGAAGGTATTCGTTATGCGCTTCAAATCCTTGTTGAGGGCAATGGAAAGAGTTCGTTtctc > 2:300869/1‑143 (MQ=255) ctgcGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCTGCTTTGAAGCTGAGCACCTTAGGTAGGGTATATCCGGAGGTAGTGTTGATGAGCTGCAAATCATTGTTGAGGGCAAGGGAAAGAGTACGTTTTTCCGCTTc > 2:138284/1‑143 (MQ=255) tgccgcgcgTTACAGGGTGGTGTTTAGCTACATCCTCCATCAAGCTGAGCACCGTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAAGCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATc < 2:304418/138‑1 (MQ=255) caCCACGCTGTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATc > 1:517125/2‑143 (MQ=255) caCCACGCTGTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATc > 1:655193/2‑143 (MQ=255) caCCACGCTGTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATc > 2:162836/2‑143 (MQ=255) caCCACGCTGTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAACACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATc > 1:654989/2‑143 (MQ=255) aCCACGCTGTAAAGCGTGGTGTTTCACCCCGTTTTCCTTCAAGCGTAGCACCTTAGGTAGGGTATAGCCCAATGGAAATCTGATTAGCGCCAAAGCAGTGCTCAGGGCAATGGAAAGAGTACGTGGCTCCCCTTCAGAAATcc > 2:399391/1‑143 (MQ=255) ccACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCa < 2:151641/143‑1 (MQ=255) cACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGGTTCTCCCCCTCAGAAATCCAc > 2:70678/1‑143 (MQ=255) aCGCTTTACAGCGGGGTGTTTAACCACATCCTCCATCAAGCTGAGCACCGTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACt < 1:760278/143‑1 (MQ=255) cGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCAccg > 1:295322/1‑141 (MQ=255) | TTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTG > NC_000913/2761480‑2761758 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |