| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,814,174 | T→A | 11.7% | intergenic (+20/+44) | emrB → / ← luxS | multidrug efflux pump membrane subunit EmrB/S‑ribosylhomocysteine lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,814,174 | 0 | T | A | 11.7% | 130.3 / 8.8 | 52 | intergenic (+20/+44) | emrB/luxS | multidrug efflux pump membrane subunit EmrB/S‑ribosylhomocysteine lyase |
| Reads supporting (aligned to +/- strand): ref base T (19/26); new base A (2/4); total (22/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01 | |||||||||||
GATTATTTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATC > NC_000913/2814034‑2814313 | gctgagttCGGCCAATGAGAGCCTCTGGATGTCAGCCGGGATAGTCCTCGCCCGGCTGGGGCTGGTGTGGGTTGCTAAACCGCCATTTGGCGCAGGTGGCGGTGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAttt < 1:321694/137‑1 (MQ=255)gaggtcttCCGCCCAGGGTATCTTCTGGATGTCAGCCGGGAGATGCCTCGTCCTGCGGGGGCTGGTGTGGGGTGCTACACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAttt < 2:563965/137‑1 (MQ=255) ttattTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGa < 1:130263/143‑1 (MQ=255) tattTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGaa < 2:753841/143‑1 (MQ=255) tCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTgg < 1:208320/143‑1 (MQ=255) ccGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGc < 2:172237/143‑1 (MQ=255) ccAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCtta < 1:61809/143‑2 (MQ=255) cAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCtttt > 1:766287/1‑143 (MQ=255) gagaTCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCa < 1:776872/143‑1 (MQ=255) gagaTCTTCTGGATGGCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCa < 1:27880/143‑1 (MQ=255) gaTCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAAGTGGCTTTTTTCaat > 2:697053/1‑143 (MQ=255) ttctGGCTGTCAGCCGGGGTATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTTTTCTGGCTTTTTTCaattaat < 2:497339/143‑1 (MQ=255) ttctGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTACGTGCAACTAAGCCAGTTCAGTTGAACTGGCCTTTTTCaattcat > 2:783842/1‑143 (MQ=255) ttctGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCaattaat < 1:621847/143‑1 (MQ=255) ttctGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCtt > 1:33174/1‑131 (MQ=255) ttctGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCtt < 2:33174/131‑1 (MQ=255) ctGGATGTCAGCCGGGATATTCCTCGCCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATtg < 1:396498/143‑1 (MQ=255) gtctGTCTGCCGCGATACTCTTCGTCCTGCTCTGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGCGGCGGCGTATTCGGTGCGCACTAATTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATtgtg < 2:595280/140‑1 (MQ=255) ggATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGGGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATtgtg < 1:323152/143‑1 (MQ=255) ccGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGtt < 1:752121/143‑1 (MQ=255) gATATCCCTCGGCCTGCTGGGGCTGGTGTGGGTTGCTGAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACt < 2:817191/143‑1 (MQ=255) atTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAATAGCCATGTGGCCGATGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGAc < 2:575273/143‑1 (MQ=255) ttCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACGACTAAGGCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACt < 1:207058/143‑1 (MQ=255) ccTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGCTCATTTGACCTGGCTTTCTTCCATCAATTGTGAATATCGTTGAATGACTac > 2:273737/1‑142 (MQ=255) gTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGAtgt > 1:759452/1‑143 (MQ=255) ctgctgGGGTTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCa < 1:525821/143‑1 (MQ=255) gctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGt > 1:689790/1‑143 (MQ=255) ggTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCTGCGGAGGCTGTTCGCACTAAGTACAACTAAGCTAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAAc < 1:250445/143‑1 (MQ=255) tgGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTtct < 2:671845/143‑1 (MQ=255) ggattggCTAAACGGCCACTTGGGGAAGGTGGCGGCGGAGGCGGTGCGCACGAAGTACAATGAAGCCAGTTCATTTGAACGGGCTTTGTTCAATTAAGTGTGAAGCTAGGGTACTGACTAGACGTGCAGTTCCTGCAACTtct < 2:22170/137‑1 (MQ=255) tttGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCtt < 1:168646/143‑1 (MQ=255) tAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCCATTAATTGTGCAGCTAGTTTACTGACTAGATGTGCAGTTGCTGCGGCTTCTCTTTCGGc > 1:226674/1‑143 (MQ=255) tAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGc > 1:239131/1‑143 (MQ=255) tAAACCGCCATTTGGCGCAGGAGGCGGCGGAGGCGGTGCGCACTAAATACAACTAAGCCAAATCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGc > 1:665178/1‑143 (MQ=255) aCCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATGGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGt < 2:517881/143‑1 (MQ=255) cGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGc > 1:774416/1‑143 (MQ=255) ccATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCa > 2:211637/1‑143 (MQ=255) gcgcAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGttcttc < 1:779001/143‑1 (MQ=255) cggTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTg < 2:487156/142‑1 (MQ=255) gcggcgGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGGGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGtt > 1:750967/1‑143 (MQ=255) cggAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATg < 2:523456/143‑1 (MQ=255) ggTGCGCACTAAGTACAACTAAGCCAGATCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCGCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGGGTACGt > 2:734786/1‑143 (MQ=255) cgcACTAAGTACAACTAAGCCAGGTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTCGATGTGCAGTACCTGCAACTTCTCTTTCGGCCGTGCCAGTGCGTCGTTGCTGTTGATACGTACGTCACg > 2:721883/1‑143 (MQ=255) cTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTcc > 1:735663/1‑143 (MQ=255) tAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATCGTGAAGAAAGATTACTGACTAGCTGTGCAGTTCCTGCAACTTCTCTTTCGGCAGCGCCAGTGCTTCGTTGCGGTTGATGCGTCCGTCACGCTCCa > 2:585876/1‑143 (MQ=255) gTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGaa < 2:185656/143‑1 (MQ=255) aaCTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCt < 2:257990/143‑1 (MQ=255) aCTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTCCGTTGCTGTTGATGCGTACGTCACGTTCCACGATGCtt > 2:642984/1‑142 (MQ=255) tAAGCGAGGTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTAcg < 2:472942/143‑1 (MQ=255) aaGCCTGTTCATTTTAACTGGCTTTTTTCATTTAATTGTGAAGATAGTTTACTGACTAGTTGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTAcgc < 2:215302/143‑1 (MQ=255) ccAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATCCGTACGTCCCGTTCCAGAAGCCCACgcgcc > 2:104889/1‑142 (MQ=255) ccAGTTCAAATGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATCCTACgcgcc > 2:29180/1‑142 (MQ=255) ttCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAatat > 1:105393/1‑143 (MQ=255) tCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTAATGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTCCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATc > 1:99601/1‑143 (MQ=255) | GATTATTTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATC > NC_000913/2814034‑2814313 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |