| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,041,632 | T→A | 100% | V107E (GTG→GAG) | ygfZ → | folate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,041,632 | 0 | T | A | 97.5% | 100.4 / ‑4.7 | 40 | V107E (GTG→GAG) | ygfZ | folate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (17/22); minor base C (1/0); total (18/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.08e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGAC > NC_000913/3041495‑3041758 | ccATTGCGACGCCAAAGGTAAAATGTGGAGCAATGTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCa < 1:54089/143‑1 (MQ=255) gCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCCTGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGACCAAATATGCGGCATTCTCTAAACAGGCCATCGCGCCAga > 1:63124/1‑143 (MQ=255) gTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTACAGAGACCATCGCGCCAGACGACGAg > 2:558820/1‑143 (MQ=255) aaaTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCgt < 2:344424/143‑1 (MQ=255) aaaTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGAAGATTTCGTTAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATCCTCTCAAGCGACCATCGCGCCAGCCGACGAGcct > 2:135236/1‑141 (MQ=255) tgtgGAGCAATTTACGTCTGTTCCGCGACGGCGATAGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGGCTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCTGGCCAGACGACGAGCGtgtg > 2:311865/1‑143 (MQ=255) tgGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGtgct < 1:391367/143‑1 (MQ=255) aGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTgctgct > 1:664831/1‑143 (MQ=255) aTTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgt < 1:531678/143‑1 (MQ=255) aCGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAAGATGCGGTATTCTCGAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTg > 1:772641/1‑143 (MQ=255) cGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGc > 1:569331/1‑143 (MQ=255) tCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCACGACCACTTGCTTGTGCTGCTTGGTGTTGCCGGtttt < 1:155927/143‑1 (MQ=255) cGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgcgcg < 2:131003/143‑1 (MQ=255) gATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGCGCTGCCGGTCTTCAGgcccgcgc > 1:760019/1‑143 (MQ=255) tttGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCt > 1:106187/1‑143 (MQ=255) tttGCATGGACTGAACGGCGCAGCGTGCGTGACCCGCAGCGCACGGAACGTAAACAATATGCGGTATTGTCGAAAGAGACCATCGCGCCAGACGACGCGCGTGTGCTGATTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCt < 1:754764/143‑1 (MQ=255) ttGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTg > 1:431794/1‑143 (MQ=255) tGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATGCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCaaa < 1:402570/143‑1 (MQ=255) tGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATctct < 2:741495/143‑1 (MQ=255) aaCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTATTCACGCGCGCGCCGCGCTGGCCAATCCCttc > 2:230598/1‑142 (MQ=255) aCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGTCAGCCGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTa < 2:327076/143‑1 (MQ=255) aCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCCCGCTGGCAAAGCTCCTTa > 1:107522/1‑143 (MQ=255) aCGGCGCAGCGCGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTa < 1:505659/143‑1 (MQ=255) gcgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGCTATTCTCTAAAGAGACCATCGCGCCAGTCGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCtt < 2:281773/143‑1 (MQ=255) aaCCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCTGCGGACGCGCGTGTGCCGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCCCTTTAGCGAACTGCCTCCgaaa > 2:97163/1‑143 (MQ=255) cGCAGCTGGCTGAACTGAAAAAAGATGCGGTATGCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaagaa < 1:304362/143‑1 (MQ=255) gCTGACTGAACTGAAGAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAAc < 2:636729/143‑1 (MQ=255) ttactgaactcaAAAAATATGCGGTATTCTCTAAAGAGTCCATCGCGCCAGACGACGAGCGTGTGCTGCCTTGCCTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAg < 2:325457/141‑1 (MQ=255) actgaactgaAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCACATCTCTTTAGCGAACTGCCTTCGCAAGACACACAgca > 2:536686/1‑141 (MQ=255) tgaactgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAACAACAGGTAg > 1:652381/1‑143 (MQ=255) ctgaaAAACTATGCGGTATTCTCTCAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCACTGGCAAATCTCTTTAGCGAACTCCCTTCAAAAAAAAAACAGGTAGTCaa < 2:294030/143‑1 (MQ=255) tgagAAAAGAGGCGGTATCGTCTAAAGAGACCATGGCGCCAGACGACGAGCGTGTGCTGCGTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCaaa < 2:345943/143‑1 (MQ=255) tgaaAAAGTATGCGGATTTCTCTAAGGAGTCGCTCTCGGCAGAGGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGAGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCaaa < 2:580493/143‑1 (MQ=255) aaaaaaTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGGAGTCAaaga > 1:231968/1‑143 (MQ=255) aaaaaaTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCGTCGCAAGAAAACCAGGTAGTCAaagc > 2:501725/1‑142 (MQ=255) aaaaaTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATGTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaagaa < 2:654507/143‑1 (MQ=255) aaaaaTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaagaa < 1:639688/143‑1 (MQ=255) caaaGATGCGGTATTCTCTAAAGAGACCACCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGCTGCCGGTTCTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaagaag < 1:18405/142‑1 (MQ=255) tGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGa < 1:532876/143‑1 (MQ=255) gCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGCACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGAc > 2:638136/1‑143 (MQ=255) | CCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGAC > NC_000913/3041495‑3041758 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |