Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,404,619 A→G 9.6% intergenic (+161/‑817) yhdH → / → accB acrylyl‑CoA reductase/biotin carboxyl carrier protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,404,6190AG 9.6% 111.4 / 3.8 49intergenic (+161/‑817)yhdH/accBacrylyl‑CoA reductase/biotin carboxyl carrier protein
Reads supporting (aligned to +/- strand):  ref base A (28/13);  new base G (3/2);  total (34/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.51e-01

AAGAGAGGGAACTCATTTTGAAATCATTATTCAATCGGTTAACGGGAAAAGCGGTTAGCCGGACAGCTTTCGTCGAACACCTTGGTCAGGAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAGCCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATT  >  NC_000913/3404478‑3404758
                                                                                                                                             |                                                                                                                                           
tagggagGGAACTCATTTTGAAATCATTATTCCATCGGTTAACGGGAAAAGCGGTTAGCCGGACAGCTTTCGTCGAACACCTTGGTCAGGAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCTACTGACCACaaa                                                                                                                                            <  2:34974/139‑1 (MQ=255)
 agagagGGAACTCATTTTGAAATCATTATTTACGCCGATTCCGAGAAAAGTGGTTAGCCGGACAGCTCTCGTCGAACACCTTGGGCAGGAAGTTGTACAACATCATCCAAACTGGAAGGTAGGGCTTTCGAGTGACCACAAAt                                                                                                                                           >  2:98083/1‑143 (MQ=255)
   agagGGAACTCATTTTGAAATCATTATTCAATCGGTTAACGGGAAAAGCGGTTAGCCGGACAGCTTTCGTCGAACACCTTGGTCAGGAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTg                                                                                                                                         >  1:113522/1‑143 (MQ=255)
     agGGAACTCATTTTGAAATCATTATTCAATCGGTTAACGGGAAAAGCGGTTAGCCGGACAGCTTTCGTCGAACACCTTGGTCAGGAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGAt                                                                                                                                       <  2:503619/143‑1 (MQ=255)
       ggAACTCATTTTGAAATCATTATTCAATCGGTTAACGGGAAAAGCGGTTAGCCGGACAGCTTTCGTCGAACACCTTGGTCAGGAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACACATTGATgc                                                                                                                                     >  1:760348/1‑143 (MQ=255)
            tCATTTTGAAATCATTATTCAATCGGTTAACGGGAAAAGCGGTTAGCCGGACAGCTTTCGTCGAACACCTTGGTCAGGAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCAtt                                                                                                                                >  2:352654/1‑143 (MQ=255)
             cATTTTGAAATCATTATTCAATCGGTTAACGGGAAAAGCGGTTAGCCGGACAGCTTTCGTCGAACTCCTTAGTCACGAAGTTATGCAGCATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTg                                                                                                                               <  1:266370/143‑1 (MQ=255)
             cATTTTGAAATCATTATTCAATCGGTTAACGGGAAAAGCGGTTAGCCGGACAGCTTTCGTCGAACACCTTGGTCAGGAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCTACTGACCACAAATTGATTCGCATTg                                                                                                                               >  1:293673/1‑143 (MQ=255)
               ttttGAAATCATTATTCAATCGGTTAACTGGAAAAGCGGTTATCCGGACATCTTTCGTCGAACACCTTGGTCAGGAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACACATTGCTGCGCATTGAt                                                                                                                             >  2:780397/1‑143 (MQ=255)
               ttttGAAATCATTATTCAATCGGTTAACGGGAAAAGCGGTTAGCCGGACAGCTTTCGTCGAACACCTTGGTCAGGAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTAGACTGACCACAAATTGATGCGAATTGAt                                                                                                                             >  2:258737/1‑143 (MQ=255)
                tttGAAATCATTATTCAATCGGTTAACGGGAAAAGCGGTTAGCCGGACAGCTTTCGTCGAACACCTTGGTCAGGAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAGATTGATTCGCCTTGATa                                                                                                                            >  1:591725/1‑143 (MQ=255)
                     caTCATTATTCCAGCGCTGAACGGGAAAAGCGGGTAGCCGGACAGCTTTCGTCGAACACCTTGCTCAGGCAGTTATACAACAGCATCCAAACTGGAAAGTCATGGCCTAGCATCGCCATAATTTGATGCGCATTGATACTCCa                                                                                                                       <  2:669412/142‑1 (MQ=255)
                          ttactCAATCGCGTGACCGGCGAAGCGGTTAGCACGACAGCTCTCGTCGAACACCTTGGTCAGGAAGTTATACAACATCATCCAAACTTGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTaaa                                                                                                                  <  2:423212/143‑1 (MQ=255)
                                aaTCGGTTAACGGGAAAAGCGGTTAGCCGGACAGCTTTCGTCGAACACCTTGGTCAGGAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCta                                                                                                            >  2:458249/1‑143 (MQ=255)
                                     gTTAACGGGAAAAGCGGTTAGCCGGACAGCTTTCGTCGAACACCTTGGTCAGGAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAACTTGATGCGCATTGATACTCCACTAAACAGCCcttatt                                                                                                       >  2:648818/1‑143 (MQ=255)
                                      ttAACGGGAAAAGCGGTTAGCCGGACAGCTTTCGTCGAACACCTTGGTCAGGAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTg                                                                                                      <  1:710070/143‑1 (MQ=255)
                                        aaCGGGAAAAGCGGTTAGCCGGACAGCTTTCGTCGAACACCTTGGTCAGGAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGAt                                                                                                    <  2:186066/143‑1 (MQ=255)
                                         aCGGGAAAAGCGGTTAGCCGGACAGCTTTCGTCGAACACCTTGGTCAGGAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTGTTATTGATa                                                                                                   >  2:123382/1‑143 (MQ=255)
                                         aCGGGAAAAGCGGTTAGCCGGACAGCTTTCGTCGAACACCTTGGTCAGGAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATa                                                                                                   >  2:119891/1‑143 (MQ=255)
                                              aaaaGCGGTTAGCCGGACAGCTTTCGTCGAACACCTTGGTCAGGAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCt                                                                                              <  2:756673/143‑1 (MQ=255)
                                                   cGGTTAGCCGGACAGCTTTCGTCGAACACCTTGGTCAGGAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTc                                                                                         >  1:253406/1‑143 (MQ=255)
                                                      ttAGCCGGACAGCTTTCGTCGCACACCTTGGTCAGGAAGTTATACAACATCATCCAACCTGGCAAGTCATGATTTCGACTGACCACAAATTGATGCGCCTTGATACTCAACTAAACAGCTCTTCTTGATACGCCTACGTCGcc                                                                                      >  2:776126/1‑143 (MQ=255)
                                                             gACAGCTTTCGTCGAACACCTTGGTCAGGAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAgg                                                                               >  1:790525/1‑143 (MQ=255)
                                                                  cTTTCGTCGAACACCTTGGTCAGGAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTAt                                                                          <  1:269351/143‑1 (MQ=255)
                                                                   tttCGTCGAACACCTTGGTCAGGAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATg                                                                         >  1:749567/1‑143 (MQ=255)
                                                                    ttCGTCGAACACCTTGGTCAGGAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGAATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGt                                                                        >  2:401140/1‑143 (MQ=255)
                                                                        tcgAACACCTTGGTCAGGAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACATGTATATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCt                                                                    >  2:394809/1‑143 (MQ=255)
                                                                          gAACACCTTGGTCAGGAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATAAATGCGCATTGATACTCCACTAAACAGATATTATTGATACGCCTCCGTCGCATGTTAGGTTTGTGTTGCttt                                                                  >  1:456339/1‑143 (MQ=255)
                                                                           aaCACCTTGGTCAGGAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGAGACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTg                                                                 >  1:300881/1‑143 (MQ=255)
                                                                            accccTTGGTCACGAAGTTCTACCACATCATCCAAACTCGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGc                                                                <  1:292987/140‑1 (MQ=255)
                                                                                cTTGGTCAGGAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAGATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTgg                                                            >  2:658577/1‑143 (MQ=255)
                                                                                cTTGGTCAGGAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAGATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTgg                                                            >  2:657983/1‑143 (MQ=255)
                                                                                      cAGGAAGTTATACCACATCAGCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGacg                                                      <  2:566453/143‑1 (MQ=255)
                                                                                        ggAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGacgct                                                    >  1:406759/1‑143 (MQ=255)
                                                                                         gAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCACGTTAGGGCTATGTTGCTTTGCCTGGGCGacgcta                                                   >  2:439572/1‑143 (MQ=255)
                                                                                           aGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGacgctacg                                                 >  2:391974/1‑143 (MQ=255)
                                                                                            gTTATACAACATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCCCTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGTGCGacgctacga                                                >  1:425874/1‑142 (MQ=255)
                                                                                                  caacaTCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACGAAACAGCTATTATTGATACGCCTCCGTCCCCTGCTAGGTTTATGTTGCTTTGCCTGGGCGACGCTGCGCTTAGcc                                          >  2:601597/1‑143 (MQ=255)
                                                                                                   atcatcatCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGAGACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAGccc                                         <  1:580920/141‑1 (MQ=255)
                                                                                                   aacaTCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAGATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAGccc                                         <  1:658577/143‑1 (MQ=255)
                                                                                                   aacaTCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAGATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAGccc                                         <  1:657983/143‑1 (MQ=255)
                                                                                                   aacaTCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAGccc                                         >  1:116244/1‑143 (MQ=255)
                                                                                                     catcatCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTGCGCTTAGCCCct                                       >  1:83685/1‑143 (MQ=255)
                                                                                                      atcatcCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAGcacatt                                      >  2:398020/1‑138 (MQ=255)
                                                                                                       tcatcCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAGCCCctta                                     >  1:383680/1‑143 (MQ=255)
                                                                                                              aaCTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAGCCCCTTACTTAttt                              <  2:423688/143‑1 (MQ=255)
                                                                                                                    aaaGTCATGATTCCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAGCCCCTTACTTATTTCTGGTa                        <  1:35581/143‑1 (MQ=255)
                                                                                                                               ttCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAGCCCCTTACTTATTTCTGGTACCATGGGGTGa             >  1:227841/1‑143 (MQ=255)
                                                                                                                                          acaAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGATAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAGCCCCTTGCTTATTTCTGGTACCCTGGGGTGAACAATCTGGtt  >  1:546440/1‑143 (MQ=255)
                                                                                                                                          acaAATTGATGCGCATTGAAACTACAACAAACAGCTAATATTGATACGCCTCCGTCGCCTGTTAGGATTATGTTGCTTTGCCTGGGCGACGCTACGCTTAGCCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGAtt  >  2:714836/1‑143 (MQ=255)
                                                                                                                                             |                                                                                                                                           
AAGAGAGGGAACTCATTTTGAAATCATTATTCAATCGGTTAACGGGAAAAGCGGTTAGCCGGACAGCTTTCGTCGAACACCTTGGTCAGGAAGTTATACAACATCATCCAAACTGGAAAGTCATGATTTCGACTGACCACAAATTGATGCGCATTGATACTCCACTAAACAGCTATTATTGATACGCCTCCGTCGCCTGTTAGGTTTATGTTGCTTTGCCTGGGCGACGCTACGCTTAGCCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATT  >  NC_000913/3404478‑3404758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑