BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,831,761	C→G	10.7%	A435A (GCC→GCG)	yicH →	AsmA famly protein YicH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,831,761	0	C	G	10.7%	92.3 / 4.0	41	A435A (GCC→GCG)	yicH	AsmA famly protein YicH
Reads supporting (aligned to +/- strand): ref base C (19/17); new base G (3/2); total (22/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.67e-01

GCCGCAATCTGATCATTGATATCGACCCTGACTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACGCCA  >  NC_000913/3831625‑3831902

gCCGCAATCTGATCATTGATATCGACCCTGACTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATAGGGCGTCTGGAGTGGCTCGGCGACTCTGAATGCCGCCGCcgcg                                                                                                                                         >  1:760158/1‑143 (MQ=255)

 ccGCAATCTGATCATTGATATCGACCCTGACTTCCCGTGGCAGCTCACCGCGCTCGCTGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGa                                                                                                                                        <  1:146064/143‑1 (MQ=255)

           aTCATTGATATCGACCCTGACTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACCCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCAGCACTCAATCg                                                                                                                              >  2:147847/1‑143 (MQ=255)

                    atCGACCCTGACTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCACGACATTCAATCGTGTGGATGt                                                                                                                     >  1:113483/1‑143 (MQ=255)

                      cgccccTGACTTCTCTTGGTTGCTCATCGCTCTCGATCGTTACGGTGCCAACCTGACGCTGGTTACCGATCATGACTGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTtc                                                                                                                   <  2:273673/140‑1 (MQ=255)

                        aCCCTGACTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTtcgt                                                                                                                 <  2:434998/143‑1 (MQ=255)

                          ccTGACTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCGGCCGCGACATTCAATCGTGTTGATGTtcgtcg                                                                                                               >  1:490354/1‑143 (MQ=255)

                             gACTTCCCGTGGCCGCGCACCGCGCTCGGTGGTTACGGTGCCACCCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGccc                                                                                                            <  2:236300/143‑1 (MQ=255)

                              aCTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCg                                                                                                           >  1:485351/1‑143 (MQ=255)

                                ttCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGGGTGGCTCGGCGACTCTGCATGCCGCCGCCGCGACATTCAATAGTGTTGATGTTCGTCGCCCGTc                                                                                                         >  1:445250/1‑143 (MQ=255)

                                     gTGGCGGCTCGCCGCGCTCCATGGTTCCGGTGCGAGCACGACGATGGTTACCGCTCATCGGTGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGACGTGTCACCCGGTTGTGTTGCTGTTCGTCGCCCGTCGCTgg                                                                                                    <  2:619664/143‑1 (MQ=255)

                                           gCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCGCGGCGCATCTGAATGCCGCCGCCGCGACATGCAATCGTGCTGATGTTCGTCGCCCGTCGCTGGCGCTgg                                                                                              >  2:275025/1‑142 (MQ=255)

                                             tCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGCTGTTCGTCGCCGGGCGCTGGCGCGGAcc                                                                                            >  1:131125/1‑143 (MQ=255)

                                              cagcgcgCTCGATCGTTACGGTGCCATCCGTACGCGGGTCACCCATCTTAAATTGGCCGTCTGGAGTGGCTCGGCTAATCTGAATGCCGCCGCCGCAACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCg                                                                                           <  2:90310/140‑1 (MQ=255)

                                                      tCGATGGTTCCGGTGCCACCCTGCCGCTGGTTACCGATCATAACTGGGGCGTCGGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAAcagc                                                                                   <  2:350025/143‑1 (MQ=255)

                                                                      cAACCTGACGTTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATGCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCa                                                                   <  2:70224/143‑1 (MQ=255)

                                                                      cAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTAGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCa                                                                   <  1:734910/143‑1 (MQ=255)

                                                                         ccTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCACCAGCAGCACGGCGAATATCAGCg                                                                >  2:418543/1‑143 (MQ=255)

                                                                                  ggTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCACCAGCAGCACGGTGAATCGCACCGAACTGAggg                                                       >  1:653776/1‑141 (MQ=255)

                                                                                         gATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGCTGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAACGGACTGAGTGCATTTAc                                                >  2:582389/1‑143 (MQ=255)

                                                                                               acaTGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGaaaa                                          <  1:422538/141‑1 (MQ=255)

                                                                                                  tGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTCGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAgg                                       <  1:150809/143‑1 (MQ=255)

                                                                                                     ggCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGCTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATACCAGCGAACTGAGCGCACCTACTGAAAACGGCAt                                    >  2:382941/1‑143 (MQ=255)

                                                                                                          cTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCagaccgg                               >  1:458808/1‑137 (MQ=255)

                                                                                                            ggcgTGGCTCGGCGAGTCTGCGTGCGGCGCCCGCGAGATTGAACCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCGAACAGCAGCACGGTGAATATCAGGGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGaa                             <  2:334921/140‑1 (MQ=255)

                                                                                                            ggAGTGGCTCGGCGGAGCGGGACGCCGCGGCCGGGGCATGCACTCGGGTTGGTGTTCGGCGGGCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGaa                             <  2:55746/143‑1 (MQ=255)

                                                                                                            ggAGGGGCTCGGCGAACCGGAATGCCGCCGCCGCGACATTTAATCGGGTTGATGCGCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGAACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGaa                             <  2:391232/143‑1 (MQ=255)

                                                                                                              ggTGGCTCGGCGAATCTGAATGGCGCCGCCGCGACATGCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGc                           <  1:703531/142‑1 (MQ=255)

                                                                                                               gTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGcc                          >  1:392653/1‑143 (MQ=255)

                                                                                                                gtgttCGGCGAATCGGAATGCCGCCGGCGCGACATCCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCa                         <  1:739742/139‑1 (MQ=255)

                                                                                                                 ggCTCGGCGAATCGCGCTCCCGCCGCCGCGACATTCAATCGTGTTGATGCTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCAc                        <  2:816445/143‑1 (MQ=255)

                                                                                                                   ctctGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCg                      <  2:225641/143‑1 (MQ=255)

                                                                                                                   cTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGGAGCCAcag                      >  1:313012/1‑141 (MQ=255)

                                                                                                                    tCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGGGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGc                     >  2:737472/1‑143 (MQ=255)

                                                                                                                    tCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGCGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGc                     >  2:642240/1‑143 (MQ=255)

                                                                                                                    tCGGCGAACCTGACGGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGc                     <  2:614013/143‑1 (MQ=255)

                                                                                                                         gAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCACCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAgtg                >  1:189030/1‑143 (MQ=255)

                                                                                                                         gAATCTGAATGCCGCCGCCGCGACATTCAATAGTGTTGATGTTCGTCGCCCCTCGCTGGCGCTGACCGCCAACAGAAGCACGGAGAATAACAGCGAGCTGAGTGCATTTAGTGAAAAAGGCATACTGGAAGACACCGcccgag                >  2:241869/1‑139 (MQ=255)

                                                                                                                           gattGAATGTCGCGGCGGCGGCATTCAATCGTGTTGATGCTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGtt              >  1:699522/4‑143 (MQ=255)

                                                                                                                             ttGAATGTCGCGGCGGCGGCATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTc            >  1:116136/2‑143 (MQ=255)

                                                                                                                             cTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCCCCAGTGTTTc            >  1:499588/1‑143 (MQ=255)

                                                                                                                                   gccgccgccGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAAc      <  1:729254/143‑1 (MQ=255)

                                                                                                                                       ccgccgcGCCATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCCCTGACCGCAAACAGCAGAACGGTGAACATCCGCGAACTGAGCGCATAGCCTGAGAACGGAATGCAGCAAGCAACCGACCGTGTTTCCCAACCGCca  >  2:357311/1‑143 (MQ=255)

GCCGCAATCTGATCATTGATATCGACCCTGACTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACGCCA  >  NC_000913/3831625‑3831902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`