| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,915,227 | G→T | 10.7% | intergenic (‑27/+326) | glmU ← / ← atpC | fused N‑acetylglucosamine‑1‑phosphate uridyltransferase and glucosamine‑1‑phosphate acetyltransferase/ATP synthase F1 complex subunit epsilon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,915,227 | 0 | G | T | 10.7% | 115.1 / 4.3 | 46 | intergenic (‑27/+326) | glmU/atpC | fused N‑acetylglucosamine‑1‑phosphate uridyltransferase and glucosamine‑1‑phosphate acetyltransferase/ATP synthase F1 complex subunit epsilon |
| Reads supporting (aligned to +/- strand): ref base G (23/18); new base T (2/3); total (25/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01 | |||||||||||
GCTGAACCATCGCTTTCCCGGCAAGGGTATGCAGCACTTTCGGAAGATCGGAATACATGCGCGTGCCTTTGCCTGCGGCAAGGATCACTACGCTCATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCCATTTTCGACCATCGTCACGCCTTTTGTCTGCAGAAAA > NC_000913/3915089‑3915366 | gCTGAACCATCGCTTTCCCGGCAAGGGTATGCAGCACTTTCGGAAGATCGGAATACATGCGCGTGCCTTTGCCTGCGGCAAGGATCACTACGCTCATAGCATTATTCAACATACGCGTCCCGACTGCAATTTGCGCACGAAtt > 2:801409/1‑143 (MQ=255) aCCATCGCTTTCCCGGCAAGGGTATGCAGCACTTTCGGAAGATCGGAATACATGCGCGTGCCTTTGCCTGCGGCAAGGATCACTACGCTCATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAAc < 1:94318/143‑1 (MQ=255) cATCGCTTTCCCGGCAAGGGTATGCAGCACTTTCGGAAGATCGGAATACATGCGCGTGCCTTTGCCTGCGGCAAGGATCACTACGCTCATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCg > 2:657092/1‑143 (MQ=255) aTCGCTTTCCCGGCAAGGGTATGCAGCACTTTCGGAAGATCGGAATACATGCGCGTGCCTTTGCCTGCGGCAAGGATCACTACGCTCATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGc > 2:463482/1‑143 (MQ=255) gtccccGGCAAGTGTATGCAGCGCTTTCGGAAGATCGGAATACATGCGCGTGCCTTTGCCTGCGGCAAGGATCACTACGCTCATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCAc < 2:372135/140‑1 (MQ=255) ttCCCGGCAAGGGTATGCAGCACTTTCGGAAGATCGGAATACATGCGAGTGCCTTTGCCTGCGGCAAGGATAAGTAAGCTAATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCAcc > 2:133127/1‑143 (MQ=255) ccGGCAAGGGTATGCAGCACTTTCGGAAGATCGGAATACATGCGCGTGCCTTTGCCTGCGGCAAGGATCACTACGCTCATAGCATTATTCAACATACGCGTCCTGACTGTGATTTGAGAACGAATTTAAACGGCTTCACCtgg > 1:53027/1‑141 (MQ=255) ccGGCAAGGGGATGCAGCACGTTCGGAAGATCGGAATACATGCGCGTGCCTTTGCCTGCGGCAAGGATCACTACGCTCATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGCATGAATTTAAACCGCTTCACCTTg < 2:716265/143‑1 (MQ=255) gcaCTTTCGGAAGATCGGAATACATGCGCGTGCCTTTGCCTGCGGCAAGGATCACTACGCTCATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGCAAAAACTACAttttt > 1:714457/1‑143 (MQ=255) tttCGGAAGATCGGAATACATGCGCGTGCCTTTGCCTGCGGCAAGGATCACTACGCTCATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACTAATTTAAACCGCTTCACCTTGAAGAAACTACATTTTTTTCa > 1:671557/1‑143 (MQ=255) tttCGGAAGATCGGAATACATGCGCGTGCCTTTGCCTGCGGCAAGGATCACTACGCTCATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACTAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCa > 1:142828/1‑143 (MQ=255) gcaGATCGGAATACATGCGCGTGCCTTTGCCTGCGGCAAGGATCACTACGCTCATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTg < 2:258422/141‑1 (MQ=255) gAAGATCGGAATACATGCGCGTGCCTTTGCCTGCGGCAAGGATCACTACGCTCATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTg > 1:415492/1‑143 (MQ=255) aaGATCGGAATACATGCGCGTGCCTTTGCCTGCGGCAAGGATAACTACGCTCATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTGTTTTCATCGTGa > 2:406216/1‑143 (MQ=255) aGATCGGAATACATGCGCGTGCCTTTGCCTGCGGCAAGGATCACTACGCTCATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTGaa < 2:293792/143‑1 (MQ=255) aGATCGGAATACATGCGCGTGCCTTTGCCTGCGGCAAGGATCACTACGCTCATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTGaa < 1:523293/143‑1 (MQ=255) aTCGGAATACATGCGCGTGCCTTTGCCTGCGGCAAGGATCACTACGCTCATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTGAAAt < 1:21551/143‑1 (MQ=255) cGGAATACATGCGCGTGCCTTTGCCTGCGGCAAGGATCACTACGCTCATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTGAAATgg > 2:503452/1‑143 (MQ=255) aTGCGCGTGCCGTTGCCTGCGGCAAGGATCACTACGCTCATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTGAAATGGACAGAGGa < 1:283338/143‑1 (MQ=255) gcgcGTGCCTTTGCCTGCGGCAAGGATCACTACGCTCATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTGAAATGGACAGAGGATa < 1:657092/143‑1 (MQ=255) tGCCTTTGCCTGCGGCAAGGATCACTACGCTCATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTGAACTGGACAGAGGATAAATTg > 1:609397/1‑143 (MQ=255) cTTGGCCTGCGGCAAGGATCACTTCGCTCGTAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTc < 2:199893/143‑1 (MQ=255) tGCCTGCGGCAAGGATCACTACGCTCATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAAt > 2:317261/1‑143 (MQ=255) cGGCAAGGATCACTACGCTCATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa < 2:197609/143‑1 (MQ=255) cGGCAAGGATCACTACGCTCATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa < 2:610457/143‑1 (MQ=255) ggCAAGGATCACTACGCTCATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTTAAATGGACAGAGGCTAAATTGTGCAATAACGGAt > 2:240648/1‑143 (MQ=255) gCAAGGGTCACTACGCTCATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACACTTTTTTCATCGTGAAATGCCCACAGGATAAATTGCTCAATAACGGAtc > 2:793758/1‑142 (MQ=255) tACGCTCATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGa < 1:89803/143‑1 (MQ=255) cTCATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaa < 2:139377/143‑1 (MQ=255) cTCATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaa > 1:542265/1‑143 (MQ=255) ccATAGCATTATTCGACATACGCGTCCTCACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaaa < 2:269320/142‑1 (MQ=255) aTAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgc > 2:372812/1‑143 (MQ=255) ttCAACATACGCGTCCTGACTGTAATTTGGGAACGAATTTAAACCACTTCACCTTGAAAAAACTACATTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGACAGCGCCACTTTc > 1:652115/1‑143 (MQ=255) cAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCCATTTTCGa < 1:471790/143‑1 (MQ=255) aaCATACGCGTCCTGACTGTAATTTGAGAACGACTTTAAACCGCTTCACCTTGAAAAAACTACATTTATTTCATCGTGAAATCGACAGAGGATAAATTGTTCAATAACGGATTATCACCGCTCACGAAAACGCCAtctgaccc > 2:642288/1‑136 (MQ=255) cgcgTCCTGACTGTAATTTGAGAACTAATTTAAACCGCTTCACCTTGAAGAAACTACATTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCCATTTTCGACCATCGt < 2:671557/143‑1 (MQ=255) cgcgTCCTGACTGTAATTTGAGAACTAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCCATTTTCGACCATCGt < 2:142828/143‑1 (MQ=255) gcgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAATGGATTATCACTGCTGACGAACGCGCCATTTTCGACCATCGTc > 2:7025/1‑143 (MQ=255) gcgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCCATTTTCGACCATCGTc > 1:127906/1‑143 (MQ=255) gcgTCCTGACTGTAATTTGAAAACGAATCTACACAGCTTCACCTTGCAAAAACTACATTTTTTTCATCGTGAAATGGACAGAGGATAAATTGCTCACTCACGCACTATCCCCGCCAACGACAGCACCATCATccaccaacacc > 2:759359/1‑138 (MQ=255) cgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCGCCGCTGACGAAAGCGCCATTTTCGACCATAGTCa > 2:56016/1‑143 (MQ=255) ccTGACTGTAATTGGAGAACTAATTGAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCCATTTTCGACCATCGTCACGc < 2:368905/143‑1 (MQ=255) gACTGTAATTTGAGAACGGATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTGAAGTGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCCATTTTCGACCATCGTCACGCCtt < 2:486269/143‑1 (MQ=255) ttGAGACCGAATTCAAACCGCTTCACCTTGAAAAAACTACACTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCCATTTTCGACCATCGTCACGCCTTTTGTCTGCa < 2:165727/143‑1 (MQ=255) ttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCCATTTTCGACCATCGTCACGCCTTTTGTCTGCa > 1:640994/1‑143 (MQ=255) aaCGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCCATTTTCGACCATCGTCACGCCTTTTGTCTGCAGaaaa > 2:564290/1‑143 (MQ=255) | GCTGAACCATCGCTTTCCCGGCAAGGGTATGCAGCACTTTCGGAAGATCGGAATACATGCGCGTGCCTTTGCCTGCGGCAAGGATCACTACGCTCATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCCATTTTCGACCATCGTCACGCCTTTTGTCTGCAGAAAA > NC_000913/3915089‑3915366 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |