| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,177,171 | T→A | 9.3% | intergenic (+43/‑187) | tufB → / → secE | translation elongation factor Tu 2/Sec translocon subunit SecE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,177,171 | 0 | T | A | 9.3% | 135.8 / 8.1 | 54 | intergenic (+43/‑187) | tufB/secE | translation elongation factor Tu 2/Sec translocon subunit SecE |
| Reads supporting (aligned to +/- strand): ref base T (24/25); new base A (2/3); total (26/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.33e-02 | |||||||||||
ATCCACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGA‑ACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTGGTTTGCCTCGCG > NC_000913/4177033‑4177311 | atgcccccGAGCGCGATGGACGACGGTCTGCGGTTCGCAATCCGTGAAGGCGGCCGTACCGTAGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGa < 1:411174/138‑1 (MQ=255)aTCCACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCACCATTTGa > 2:68667/1‑143 (MQ=255) aCCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGcc > 2:741115/1‑143 (MQ=255) ccGAGCGCGAGGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCt < 1:725034/143‑1 (MQ=255) aTCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCtttt < 2:560837/143‑1 (MQ=255) tCGCGATGGACGACGGTCTGCGCTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCttttt < 2:219844/143‑1 (MQ=255) tCGCGAGGTACGACGGTCTGCGTTTCGCAATCCGTGAAGCCGGCCGCACCGTTGGCGCAAGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCtttta < 1:302998/143‑2 (MQ=25) gcgcgACGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCtttta < 2:511853/142‑2 (MQ=255) gggtGGACGGCGGCGTGCGTGCGGCACTTCGGGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAGGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCa < 2:730201/140‑1 (MQ=37) cgATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCa > 2:813105/1‑143 (MQ=255) gttccggcgGTCTGCGTTTCGCAAGCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGc < 2:741973/136‑1 (MQ=255) gacgGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAACGGGCATCATTGGATGCCCTTTTTGCACGCTTTCg > 1:241401/1‑143 (MQ=255) gacgGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGACGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTATTTGCACGCTTTCg > 2:658326/1‑143 (MQ=255) acgGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTCGCGCAGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGt < 1:112025/143‑1 (MQ=255) gggcTGCGTTTCGTAGTCCTTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTAc < 2:605973/140‑1 (MQ=255) gTCTGCGTTTCGTAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGGTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTAcc < 1:469001/143‑1 (MQ=255) gTCTGCGTTTCGCAATCCGTGAGGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTAcc < 2:491803/143‑1 (MQ=255) tCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAGCAGTGGACGCAATGCGCACGAAAAGGGCATCGTTCGATGCCATTTTTGCACGCAATCGtgcgg > 1:214806/1‑139 (MQ=21) gCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCGCGCTTTCGTACCAGa‑a > 2:342130/1‑143 (MQ=255) gCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGa‑a > 1:554261/1‑143 (MQ=255) tttCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCCCGCTTTCGTACCAGA‑ACCt > 1:309444/1‑143 (MQ=255) ttCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTTCCGATAACATTTGCAGGCAGACGCACTAAAAGGGCATCATTCGATACCCTTTTTGCACGCTTTCGTACCAGA‑ACCTg > 1:375639/1‑143 (MQ=25) ccGCCATCCGTGAAGGCGTCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTCACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCCTACCAGA‑CCCTgg < 2:684204/142‑1 (MQ=38) gCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCCGA‑ACCTGGCt > 2:139126/1‑143 (MQ=255) cGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGA‑ACCTGGCtcatccg > 2:471766/1‑141 (MQ=255) ggtggcggcCGTGCCGTAGGCGCGGGCGTTGTAGCAAAAGTTCTTAGCTAATTGCCGATAGCATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCTCTTTTTGCACGCTTTCGTACCAGA‑ACCTGGCTCATCAGTGa < 2:143576/140‑1 (MQ=255) ggTACCGTGGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCGATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGA‑ACCTGGCTCATCAGTGATTTTCTTTg < 1:474044/142‑1 (MQ=255) cgGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGA‑ACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGc < 1:123304/143‑1 (MQ=255) gccggggaGCAAAAGTTCGGAGCTAAGTGCCGATAACATTTGACGCAATGCGCACTAAAAGGCCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGA‑ACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTga < 1:96088/136‑1 (MQ=255) ttGTAGCAAAAGTTCTTAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATTTATTTTTTGCACGCTTTCGTACCAGA‑ACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGAc < 1:512196/143‑1 (MQ=255) ttGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGA‑ACATGGCTCATCAGTGCTTTTCTTTGTCATAATCATTGCTGAGAc > 2:649782/1‑143 (MQ=255) gTAGCCAAAGTTCTGAGGTCAGCGCCGATAACATTTGACGCACTGGACAACCAAGGGGCAACATTTGCTGCTCTTTTTGCACGCTTTCGTACCAGACAATAGGCAATTCA‑ACATTTTCTTTGTCATACTCATTGCTGAGACAg < 2:540196/143‑1 (MQ=255) tAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATATGATGCCCTTTTTGCACGCTTTCGTACCAGA‑ACCTGACTCATCAGTGATATTCTTTGTCATAATCACTGCTGGGACaag > 2:477889/1‑141 (MQ=255) cAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCCTCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGA‑ACCTGGCTCATCAGTGCTCTTCTTTCTCATACTCATTGCTGAGACaaggtc > 2:608511/1‑138 (MQ=255) ttCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGA‑ACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGa < 2:495992/143‑1 (MQ=255) ttCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGA‑ACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCGCTGTTGa > 2:51178/1‑143 (MQ=255) tGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGA‑ACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAggg < 1:813105/143‑1 (MQ=255) gCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGA‑ACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGt < 1:667239/143‑1 (MQ=255) gCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGA‑ACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGt > 1:690307/1‑143 (MQ=255) gacGATAACATTCGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGA‑ACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATcc < 1:642693/141‑1 (MQ=255) ccGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGA‑ACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGACACAGGCTCTGTTGAGGGCGTATAATCCg > 2:30000/1‑143 (MQ=255) aTTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGA‑ACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTa > 1:5341/1‑143 (MQ=255) cttGACGCGAGGCGCACTAAAGGGGCATCATTTGATGCCCTTTTGGTACGCTTTCGTACCAGA‑ACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTaa < 2:639638/142‑1 (MQ=255) acacAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTCGCGTACCAGA‑ACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAGGCTaagccg > 2:13882/4‑139 (MQ=255) aTGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGA‑ACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGttt < 1:214641/143‑1 (MQ=255) tgagcACTCTAAGGGCATCATTTGATGCCCGTTTTGCTCGCTTTCGTACCAGA‑ACCTGGCTCATCAGGGATTTTCTTTGTCATATTCATTGCTGAGACAGGCTCTGTTGAGGGCGTATACTCCGAAAAGCTAATACGCGTTTc < 2:717028/140‑1 (MQ=255) cACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGA‑ACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTATTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGAtt > 2:621132/1‑143 (MQ=255) tAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCATA‑ACCTGGCTCATCAGTGATTTTCTTGGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGAGTATAATCCGAAACGCTAATACGCGTTTCGATGTgg > 1:174034/1‑143 (MQ=255) tAAAAGGGCATCATTTGATGCCCTTTTTGCAAGCTTTCGTACCAGA‑ACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCGGAGACAGGCTCTGTCGAGGGCGTATACTCCGAAAAGGTAATGCGAGATTCAATGTgg > 2:490625/1‑143 (MQ=255) tAAAAGGGCATCATTTGATGCCCTTTGTGCACGCTTTCGTACCAGA‑ACCTGGCTCATCAGTGATTTGCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTgg < 1:132040/143‑1 (MQ=255) aaaaGGGCATCAAATGATGCCCTTTTTGCACGCTTTCGTACCAGA‑ACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATGTGGt > 2:507566/1‑143 (MQ=255) aGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGA‑ACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTGGTTTg > 1:33485/1‑143 (MQ=255) gggCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGA‑ACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTGGTTTGc < 2:645226/143‑1 (MQ=255) gggCATCAAATGATGCCCTTTTTGCACGCTTTCGTACCAGA‑ACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATAAGCGTTTCGATTTGGTTTgg > 1:790607/1‑142 (MQ=255) catcatTTGATGCCCTTTTTGCACGCTTTCGTACCAGA‑ACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTGGTTTGCCTc > 1:745239/1‑143 (MQ=255) atcatTTGATGCCCTTGTTGCACGCTTTCGTACCAGA‑CCCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTGGTTTGCCTcg < 1:173981/143‑1 (MQ=255) tcagTTGATGCCCTTTTTGCACGCTTTCGTACCAGA‑ACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTGGTTTGACTcgc < 2:544469/143‑1 (MQ=255) catTTGATGCCCTTTTTGCACGCTTTCGTACCAGA‑ACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGACGGCGTATAATCCGCAAAGCTAATACGCCTTACGATTCGGTTTGCCtgggg > 2:641596/1‑139 (MQ=255) | ATCCACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGA‑ACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTGGTTTGCCTCGCG > NC_000913/4177033‑4177311 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |