| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,321,246 | A→G | 10.0% | M1T (ATG→ACG) † *151R (TGA→CGA) |
phnH ← phnG ← |
carbon‑phosphorus lyase core complex subunit PhnH carbon‑phosphorus lyase core complex subunit PhnG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,321,246 | 0 | A | G | 10.0% | 90.8 / 2.1 | 40 | M1T (ATG→ACG) † *151R (TGA→CGA) | phnH phnG | carbon‑phosphorus lyase core complex subunit PhnH carbon‑phosphorus lyase core complex subunit PhnG |
| Reads supporting (aligned to +/- strand): ref base A (17/19); new base G (2/2); total (19/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.91e-01 | |||||||||||
GCGGTTGCCAGCCGCGTTTGAGCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTTCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGA > NC_000913/4321118‑4321382 | gCGGTTGCCAGCCGCGTTTGAGCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAACCTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTTCCAGGGTCATGCGTTGTCTCcgc < 1:562859/143‑1 (MQ=255) gTTGCCAGCCGCGTTTGAGCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTTCCAGGGTCATGCGTTGTCTCCGCGaa < 1:126194/143‑1 (MQ=255) gggCCAGCCGCGGTTGGGCGGAGGCGGGGCGACAACCACGCCGGGCTCGCTCATGGCCTTTAACGGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTTCCAGGGTCATGCGTTGTCTCCGCGAAc < 2:638013/141‑1 (MQ=255) tGCCAGCCGCGTTTGAGCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTTCCAGGGTCATGCGTTGTCTCCGCGAAcc < 2:569770/143‑1 (MQ=255) tttGAGCTGATGCAGGGCGACAATGACGCCCGGCTCGCTCATGGCCTCTAACAGGCGGCGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAGAAGCGGTTTGCAGGGTCGTGCGTTGTCTCCGCGAACCATCGTAaagaa > 1:308852/1‑143 (MQ=255) tttGAGCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTTCCAGGGACGTGCGTTGCCCCCGCGAACCATCGTAaagaa > 1:310162/1‑143 (MQ=255) gCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGGAGCATAAAAGCGGTTTCCAGGGTCATGCGTTGTCTCCGCGGACCATCGTAAAGAAGTCGa > 1:44854/1‑143 (MQ=255) gCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTTCCAGGGTCATGCGTTGTCTCCGCGACCCATCGTAAAGAAGTCGa > 1:278658/1‑143 (MQ=255) gCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTTCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGa < 1:116325/143‑1 (MQ=255) cTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGATTCCAGGGTCATGCGTTGTCTCCGCGACCCATCGCAAAGAAGTCGAc > 1:277408/1‑143 (MQ=255) tGCAGGGCGACAATCACGCCCGGCTCGCTCATGACACATCACAGTCTGCGTAAACTGTGCTGGGCATCCTGCACGGGCAGCATAACAGCGGTTTCCATGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACACgg > 1:155078/1‑143 (MQ=255) aCGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTTCCAGGGGCATGCGATGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCg > 2:384267/1‑143 (MQ=255) gCCCGGCTCGCACATGGCACTTAACAGGCGACGAAAACTGTGCTGGGCCTCCCGCACGAAAAGCATAAAAGCGGTTTCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGc < 1:183989/143‑1 (MQ=255) ccGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTTCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACACGGCTGGCGTTCACTGCGGccc > 2:480399/1‑142 (MQ=255) ccGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCGGGGCATCCTGCGCGGGAAGCATAGAAGCGGTTCCCAGGGTCATGCGTTGGCTCCGCCAACCATCGTCAAGCAGGCGACCCGGCTGCCGTTCACTgccgccc > 2:661647/1‑142 (MQ=255) ggtcAGGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTTCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGc < 2:771381/141‑1 (MQ=255) gctcATGGCCTTTAACAGGCGCCGAAAACTGTGCGGGGCAGCCTGCACGGGAAGCAGAAAAGCGGTTTCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGc < 1:35464/143‑1 (MQ=255) tttAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTTCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGt > 1:312100/1‑143 (MQ=255) tttAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTTCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGATGGCGAACACTTCGGCCTGGCGTGCGGCGCTGCgc > 2:199331/1‑142 (MQ=255) aGGGGACGAAAACGGTGCTGGGCATCGTGCACGGGAAGCATAAAAGCGGTGTCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATa < 1:587446/143‑1 (MQ=255) aGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTTCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGCTACCCTTGGCCTGGCGTGCGGCAATGCGTGCCATa > 1:768956/1‑143 (MQ=255) cgacgaAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTTCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTAACTTCGGCCTGGCGTGCGGCAATGCGTGCCAGACgg > 1:768004/1‑143 (MQ=255) acgaAAACTGCGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTCCGAGGGTCATGCGTTGTCTGCGCTAAGCATCGTAAAGAAGTGGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTc < 2:391128/143‑1 (MQ=255) agcaCTGGGCTGGGCATCCTGCACGGGATGCATAATAGCGGTTTCCAGGGTCATGCGTTGTCTCCGCGAACCAGCGGAAAGAAGGCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGc < 1:138808/140‑1 (MQ=255) aaCTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTTCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGt < 1:657668/143‑1 (MQ=255) gtgtgCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTTCCAGGGTCATGCGTTGTCTCCGCGAACCAGCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTcc < 1:327123/142‑1 (MQ=255) cTGTGCCGGGCATCCTGCACGGGAAGCACGAACGCGGTCGCCAGGGTCATGCGGGGTCGCTGCGAACCATCGTAAACAAGTCGCCCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTcc < 1:384674/143‑1 (MQ=255) tgtgCTGGGCATGCTGCACGGGAAGCATAAAAGCGGTTTCCGGGGTCATGCGTTGTCGCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCa < 1:445056/143‑1 (MQ=255) cTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTTCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCCGCgg > 2:149887/1‑143 (MQ=255) gggCGTCCTTCACGGGACGCATACAAGCGGTTTCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCgggg < 1:458656/143‑1 (MQ=255) gggCATCCTGCACGGGAAGCATAACAGCGGTTTCCAGGCTCATGCGTTGTCTCCGCGACCCATCGTAAAGAAGTCGCCCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCgggg < 1:481169/143‑1 (MQ=255) gggAAGCATAAAAGCGGTTTCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTc > 1:373696/1‑143 (MQ=255) gCATAAAAGCGGTTTCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGCCCCGCGGGGCCATCAGGGGTGCTGaga > 2:31678/1‑141 (MQ=255) tAAAAGCGGTTTCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGt > 1:420565/1‑143 (MQ=255) aaaGCGGTTTCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGGCAGCGTCCAGCGGGGCCATAAGGGTTTCTGATAGGttt > 1:56423/1‑143 (MQ=255) gTTTCCAGGGTCGTGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTAATAAGTTTTGAAAg < 2:308852/143‑1 (MQ=255) gTTTCCAGGGTCGTGCGTTGTCTCCGCGAACCATCGTAAAGAAGGCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTAATAAGTTTTGAAAg < 2:310162/143‑1 (MQ=255) tttCCGGGGTCATGTGTTGTCGTCGCTAACCATAGTAAAGAAGTCGACCCGGCTGGCGTTCACTGCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGt < 1:387531/143‑1 (MQ=255) ccAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGc < 1:691730/143‑1 (MQ=255) aGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCga > 1:626778/1‑143 (MQ=255) | GCGGTTGCCAGCCGCGTTTGAGCTGATGCAGGGCGACAATCACGCCCGGCTCGCTCATGGCCTTTAACAGGCGACGAAAACTGTGCTGGGCATCCTGCACGGGAAGCATAAAAGCGGTTTCCAGGGTCATGCGTTGTCTCCGCGAACCATCGTAAAGAAGTCGACCCGGCTGGCGTTCACTTCGGCCTGGCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGA > NC_000913/4321118‑4321382 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |