| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,637,646 | +T | coding (5/1500 nt) | pitA → | metal phosphate:H(+) symporter PitA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,637,645 | 1 | . | T | 97.1% | 108.5 / ‑5.4 | 35 | L2L (CTA→TTA) | pitA | metal phosphate:H(+) symporter PitA |
| Reads supporting (aligned to +/- strand): ref base . (0/1); major base T (13/20); minor base A (1/0); total (14/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑C‑TACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCGC > NC_000913/3637505‑3637781 | aTTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGTATATTAAAAAAATTCTTTATTTCACTTTGCCCGCGTCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑C‑tt < 1:477767/143‑2 (MQ=255)aTTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑C‑tt < 2:228308/143‑2 (MQ=255) ttATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAACATCGCGCACCGTCCCATG‑C‑ttc > 2:257854/1‑141 (MQ=255) agagAATTAGTTTTTGAGTGAAGACGATGCTGGGGGGAAACCAAAAGAAGGCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTACAtttgt < 1:282390/143‑1 (MQ=255) gATAATTATTTTTTGAGTGCAATCCATACAGCGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTACAtttgtt < 1:659641/143‑1 (MQ=255) aGTCAACTCCATGCAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCACAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGAttt < 2:528529/143‑1 (MQ=255) gTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTg > 1:425569/1‑143 (MQ=255) aaaTCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCGTGGATTTGCAt > 1:364667/1‑143 (MQ=255) aaaTCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCAt > 1:414602/1‑143 (MQ=255) aTACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCggg < 1:420228/143‑1 (MQ=255) aGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGt > 2:433727/1‑143 (MQ=255) aaaaaaaGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGAAAATGCGACGCGTTCATGTCCTCAAAATGGCGTAACTTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGAGCTGTTATTAttgcttg > 2:303411/1‑143 (MQ=255) aaaaGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCAc > 1:567980/1‑143 (MQ=255) aGTCTATATTTCACGTTGCCCGCGCCGCGAAAGTCACTGACAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTgg < 2:5668/143‑1 (MQ=255) atatTTCGCTTTGCCCGCGCCGCGAAAGTCGCTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCtttt < 2:551941/143‑1 (MQ=255) atatTTCACTTTGCCCGCGCCGCGAGAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCtttt < 1:165366/143‑1 (MQ=255) atTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTtgt < 2:66633/143‑1 (MQ=255) tttGCCCGTGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTc < 1:153338/143‑1 (MQ=255) tttGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTGCATTTGTTTGCTGGCCTGGATTTGCATCCCGGGCTGTTATTATTGCTTTCACTGGCTTTTGTGCTGTTc > 2:230697/1‑143 (MQ=255) tttGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGACTACCGGGCGGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTc > 2:685780/1‑143 (MQ=255) tGCCCGCGCCGCGAATGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTa < 1:389695/143‑1 (MQ=255) ccgcgccgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCGGTTCTACGa > 1:105033/1‑143 (MQ=255) cgcgccgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGaa < 1:504454/143‑1 (MQ=255) cgcgccgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGaa < 1:705478/143‑1 (MQ=255) cgcgccgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGaa < 1:490159/143‑1 (MQ=255) cgcgccgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGaa < 1:281820/143‑1 (MQ=255) gcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTCATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATc < 2:232651/143‑1 (MQ=255) aaaGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAAt > 1:10675/1‑143 (MQ=255) aaGTCACTGATACTGCGCCGCGTTCATGTCGTCAAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATg < 2:6208/143‑1 (MQ=255) aaGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGTTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATg < 1:466740/143‑1 (MQ=255) gATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCAt < 2:570150/143‑1 (MQ=255) gccgcGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CATCCATTTGTTTGCTGGCCTGGATTTGCAGACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGGGCTGTTCTACGAAGTCCTCAATGGTTTGCATGacacgtc > 1:67091/1‑140 (MQ=255) cTCAAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCaa > 1:749587/1‑143 (MQ=255) cAAAATGGCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTCGCTTTTGTGCTGTTCTACGAAGCCATCACTGGGGTCCATGACACAGCCACCGCCGTGTCAAcc > 2:571489/1‑143 (MQ=255) tGGCGTAACGTCCTATGCC‑TACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTAt < 1:173285/143‑1 (MQ=255) gCGTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCt < 1:300788/143‑1 (MQ=255) gTAACGTCCTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCtat < 1:740371/143‑1 (MQ=255) cTATG‑CTTACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCCACGGTTATCTCTACCcgcgc > 2:759741/1‑143 (MQ=255) | ATTATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑C‑TACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCGC > NC_000913/3637505‑3637781 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |