Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative colanic acid biosynthesis glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	100.0%	101.8 / NA	34	I204S (ATC→AGC)	wcaA	putative colanic acid biosynthesis glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (15/19);  total (15/19)

GTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT  >  NC_000913/2132654‑2132925                                                                                                                                      |                                                                                                                                           gTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAGGTGGAAGTACCCGGAGAGTTTTTTCGGTGACGAGGTGATGTGCATGTCACCGTGATTGATATGCAGGCTCTGCGTCg                                                                                                                                   >  1:207186/1‑143 (MQ=255) gTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTCGTCTTTGTGCTTGCGGTAAAAGTGGGAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGCGATCGATATGCAGGCTCTGcaccc                                                                                                                                   >  2:66855/1‑139 (MQ=255)      gggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGCAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCctct                                                                                                                              >  1:538907/1‑143 (MQ=255)        ggaggcAGGTGATATTTTCTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCTGAGCGGTTTTTCGGTGACGAGGGGATCTGCCTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtcttc                                                                                                                            <  2:724212/138‑1 (MQ=255)          aaaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa                                                                                                                          <  1:578696/143‑1 (MQ=255)               gCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTTCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTc                                                                                                                     <  1:507727/143‑1 (MQ=255)                           tGCCGGCGCGGTCGAATTTGTCTTTGTGCTCGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg                                                                                                         <  1:240388/143‑1 (MQ=255)                                 cgcgTTCGTTTTTGTCGTTGTGGTTGCAGTAAAAGTGGTAGTTCGCGGAGAATTTTGTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTcc                                                                                                   <  2:131222/143‑1 (MQ=255)                                           tttGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCGTCTACTTTCCACCGTTCGCCGTACTCCACCACCATcc                                                                                         >  2:431203/1‑143 (MQ=255)                                            ttGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGTTGACGTGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATCCg                                                                                        >  1:482710/1‑143 (MQ=255)                                              gTCTTTTGGCTCGCGGTAAAAGCGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCa                                                                                      <  1:63156/143‑1 (MQ=255)                                              gTCTTTGTGCTTGCGGTAAAAGTGGAAGTACAAAGAGAATTACTTATGTGACGGGGTGATCTGCATTGCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGCACTCCACCACCATCCGCa                                                                                      >  1:323873/1‑143 (MQ=255)                                               tCTTTGTGTTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATAGGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAg                                                                                     <  1:30105/143‑1 (MQ=255)                                                  ttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATAGATATGCAGGCTCTGCGTCGCCTCTTCTCCTTTCCACGGTTCGCCGTCCTCCCCCACCATCCGCgggca                                                                                  >  1:106662/1‑141 (MQ=255)                                                       cTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGACGCGATCTGCATTTCACCTTGATTGATATGCAGGCTCTGCGTCGCCCGTTTGTTTTTCCACCGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGt                                                                             >  1:554269/1‑143 (MQ=255)                                                                          tccccGGAGAATTCTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCttt                                                          <  1:310822/141‑1 (MQ=255)                                                                                gagaGTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTGTCCTCTTGTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTc                                                    <  2:673351/143‑1 (MQ=255)                                                                                 agagTTTTTCCGGTGGCGAGGTGATCTGCATTTCACCGCGATTGATCTGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg                                                   <  1:233600/143‑1 (MQ=255)                                                                                    cttttctCGGTGCCGTGGTGATCTGCCGTCCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCTCGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTa                                                <  2:382104/142‑1 (MQ=255)                                                                                      ggtggcGGGGACGAGGGGATCTGCATGTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCTGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATc                                              <  1:455688/138‑1 (MQ=255)                                                                                        tttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGa                                            <  1:564410/143‑1 (MQ=255)                                                                                         ttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCgca                                           >  1:87647/1‑141 (MQ=255)                                                                                         gggggTGACGGGGGGATCGGCGTTGCGCCGTGATTGGCGGGCAGGCTCTGCGGCGCCGCTTCTACGTTCCACGGTTCGCCGTGCTCCACCCCCGTCCGCAGGAAGATGTCGTAAGCCTGGGCCGCTGTCAGTTCGGTATCGaa                                           <  2:31487/140‑1 (MQ=255)                                                                                             gTGACGAGGTGATCTGCATTGCACCGTGATTGATATGCAGGCTCTGCGTCGCCTATTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa                                       <  1:342226/143‑1 (MQ=255)                                                                                                aCGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGATACCGaactgaaag                                    <  1:199385/143‑7 (MQ=255)                                                                                                           tgccggtCACCGTGATTGATCTGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACg                         <  2:78325/137‑1 (MQ=255)                                                                                                           cgCATTTCACCGTGATGGCTATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTGCTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACg                         <  2:746403/142‑1 (MQ=255)                                                                                                             cATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTATACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTGTCAGTTCGGTATCGCACAAACACCCTCTGAACCGcc                       >  1:86676/1‑143 (MQ=255)                                                                                                                ttCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGGAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGCAGCGAACCAACACTCTTTGAAACCCCATg                    >  2:595338/1‑143 (MQ=255)                                                                                                                   gcggTGACTGACATGCATGCTCCGCGCCGCCGCTTCTACGTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGccc                 <  2:636916/140‑1 (MQ=255)                                                                                                                    ccGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCACTTCGGTATCGCACAACCCCTCTTTGCAACACCATGCCCa                >  2:513353/1‑143 (MQ=255)                                                                                                                      gTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACACCATGCCCAgg              >  1:459947/1‑143 (MQ=255)                                                                                                                       tgattgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTTGTGATCCTGTGCCGCTTTCATGTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAggg             >  1:320873/1‑142 (MQ=255)                                                                                                                                  cAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTTTTTGAGACCCCGTGCCCAGGTAAAGACGTgga  >  1:24676/1‑142 (MQ=255)                                                                                                                                      |                                                                                                                                           GTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT  >  NC_000913/2132654‑2132925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑