Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,661,969	C→G	V55L (GTT→CTT)	iscR ←	DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,661,969	0	C	G	100.0%	59.0 / NA	24	V55L (GTT→CTT)	iscR	DNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (16/8);  total (16/8)

CCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAG  >  NC_000913/2661830‑2662108                                                                                                                                            |                                                                                                                                            ccgccCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACTGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATACCCACCGCCTGGTCCACGTACGCTGGAAAGCAg                                                                                                                                          >  1:14613/1‑143 (MQ=255)  ggccCTGGCAGCCGCCTATACCCTGACACCGGGTGGCATCTACAGATTCGAAAACGGCGCTAATCACTTCGCAAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGa                                                                                                                                         <  1:187841/142‑1 (MQ=255)                       ccTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCATACCATTTTTACGCAGACGGGaa                                                                                                                    >  2:495667/1‑143 (MQ=255)                       ccTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATCACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACgggga                                                                                                                    >  2:487336/1‑141 (MQ=255)                       ccTGACAACGGGTGGCATCTACAGAAAAGACAAAAGAAATAATGTCTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGaa                                                                                                                    >  1:146302/1‑143 (MQ=255)                             aaCGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTGGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAgt                                                                                                              <  2:327815/143‑1 (MQ=255)                                          tACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGCTAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGCAAAACAGTTGTTCCCGATCAg                                                                                                 >  2:168622/1‑143 (MQ=255)                                          tACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATACCCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTGCCAGCTacg                                                                                                 >  2:116561/1‑141 (MQ=255)                                                    tCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATCTTTCCACAGATCAAGTAACAGTTGTCCCAGATAAGAAAGAGaaag                                                                                       >  2:335442/1‑142 (MQ=255)                                                      aaCGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATACGAAAGGGAAATTc                                                                                     >  2:291186/1‑143 (MQ=255)                                                         ggCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCTCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCt                                                                                  <  2:34722/143‑1 (MQ=255)                                                              tAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATACCCACCGCCTGGTCCACGTCCGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGAGAAATTCCCTGACGt                                                                             >  1:122406/1‑143 (MQ=255)                                                              tAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGCCCACCTTGAAGTATTCGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGt                                                                             >  1:757959/1‑143 (MQ=255)                                                              tAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGt                                                                             >  1:708011/1‑143 (MQ=255)                                                                  tACGTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCgg                                                                         <  1:32742/143‑1 (MQ=255)                                                                    cTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaa                                                                       >  1:610936/1‑143 (MQ=255)                                                                        gCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAGCGCAGCCCATTTTTACGCCGACCGGAAAACAGTTGTTCCCGATAAGAAAGGGAAATTCCCTGCCGTTCGGAAatat                                                                   >  1:505585/1‑143 (MQ=255)                                                                          cAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCGCGTCCGCGGGAAAGCAGACCATTTTTACGCAGACGGGGAGACAGGCGGTCCAGATAAGAAAGGGAAATTCGCTGACGTTCGGAAATATCa                                                                 >  2:303020/1‑143 (MQ=255)                                                                               gCGATGTGGCTGGAATCTTTGCCTAACAGATAACCACCGGCTGGTCCACGCACGCTGGATAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCaa                                                            <  1:192504/143‑1 (MQ=255)                                                                                      tgctgGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGTCAACGGTAcc                                                     <  1:345319/143‑1 (MQ=255)                                                                                                           gaggaccaccGTGTGGGCGGCGTACGCGGGAAAGGAGGCCATTGTTACGGAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATGCCCTGACGTGCGGAAATATCAGCCAACGGTACCGGGCCCGCTGCAGAGTTGAgc                                <  2:552901/139‑1 (MQ=255)                                                                                                                 caccGCCTGGTCCACGTACGCAGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACg                          >  1:254416/1‑143 (MQ=255)                                                                                                                             cACGTACCCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCg              <  1:87354/143‑1 (MQ=255)                                                                                                                                         aaaGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAGTTCCCTGACGTTCGGAAATATGAGCCAACGGTACCGGCACCCCTTCAGAGTTGAGAGCAACGTCAAGCATTGCgggcagggcagat  >  2:90486/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                            CCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAG  >  NC_000913/2661830‑2662108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑