Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,041,632	T→A	V107E (GTG→GAG)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,632	0	T	A	100.0%	104.8 / NA	39	V107E (GTG→GAG)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (23/16);  total (23/16)

GCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTT  >  NC_000913/3041500‑3041763                                                                                                                                     |                                                                                                                                    gCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATcgcg                                                                                                                           <  1:548905/143‑1 (MQ=255)   gACGCCAAAGGTAAAATGTTGAGCAATTAACGTCTGTTCCGCGACGGCGATGGCACTACAAGGCTTGAACGGCCCAGCGTGCGTGAACCGCAGCTGACTGAACTGCAACAATCTGCGGTATTCCCTCAAGAGACCATcgccac                                                                                                                         >  2:127398/1‑140 (MQ=255)                  aTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCgtct                                                                                                          >  2:625930/1‑141 (MQ=255)                          cAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTg                                                                                                  <  1:137903/143‑1 (MQ=255)                          cAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACGACAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTg                                                                                                  >  1:760141/1‑143 (MQ=255)                            aTTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgt                                                                                                >  2:557613/1‑143 (MQ=255)                                 cGTCTGTTCCGCGACGGCGATGGCGTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGc                                                                                           <  2:606458/143‑1 (MQ=255)                                     tGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGt                                                                                       >  1:555612/1‑143 (MQ=255)                                       ttCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGgtt                                                                                     >  2:283549/1‑143 (MQ=255)                                          cgcgGCGGCGATGGCTTTGGATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGCAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCa                                                                                  <  1:164388/143‑1 (MQ=255)                                           gcgACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAg                                                                                 <  1:612683/143‑1 (MQ=255)                                               cgtcGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAAGATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgc                                                                             <  2:643932/140‑1 (MQ=255)                                                  cGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCCGACGACGAGCGTGTGCTGCTTGGTGTTGCCGCTTCTCAGgcgcgcc                                                                          >  2:693517/1‑142 (MQ=255)                                                     tGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGcgcgccg                                                                       <  1:72826/143‑1 (MQ=255)                                                       gCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGACAGACGACGAGCGTGTGCTGCGTGGTGTTGCCGGTTTTCAGGCGcgcgccgcg                                                                     >  1:389167/1‑143 (MQ=255)                                                         tttGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACCACGAGCGTGCGCCGCTTGGTGCTGCCGGCGTTCAGGCGcgcgccgcgcg                                                                   >  2:684874/1‑142 (MQ=255)                                                          ttGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGACAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTCTGCTGCTTCGTGCTGCCGGTTTTCAGGCGCGCGCCCCGCTg                                                                  >  2:114444/1‑143 (MQ=255)                                                          ttGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCAGATTTTCAGGCGCGCGCCGCGATg                                                                  >  1:185796/1‑143 (MQ=255)                                                            gCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGc                                                                >  1:74828/1‑143 (MQ=255)                                                              aTGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCaa                                                              >  1:736512/1‑143 (MQ=255)                                                               tGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTGGGTGATGCCGGTTTCCAGGAGCGCGCCGCGCTGGCaga                                                             >  2:350452/1‑141 (MQ=255)                                                                ggATTGGACGGTGCAGCGTTCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGTCTACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAt                                                            <  1:41535/143‑1 (MQ=255)                                                                ggATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCaact                                                            >  1:337849/1‑141 (MQ=255)                                                                ggATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGATATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTGAGGCGGGCGCCGCGCTGGCAAAt                                                            >  1:250236/1‑143 (MQ=255)                                                                 gagtGAGCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGGCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtc                                                           <  1:538800/140‑1 (MQ=255)                                                                   ttGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTATAAAGAGACCATCGCGCCAGACGAAGAACAAAAACTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtctc                                                         >  1:351067/1‑143 (MQ=255)                                                                    tggaCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAATAATATGCGGTATTCTCTAAAGAGACCGTCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTCGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATctct                                                        <  2:753656/140‑1 (MQ=255)                                                                                       gAACCGCAGCTTACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaa                                     <  1:236815/143‑1 (MQ=255)                                                                                         aCCGCAGCTGACTGAACTGAAAAAAGATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaag                                   <  1:121362/143‑1 (MQ=255)                                                                                          ccGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCCTCgaaagc                                  >  2:450547/1‑142 (MQ=255)                                                                                           cGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGGTGCTTGGTGTTGCCGGAGTTCAGGCGCGCGCCGCGCTGGCGAATCTCTTTAGCGAACTGCCTTCgagagaa                                 >  2:392713/1‑143 (MQ=255)                                                                                             cAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTACCGACCTGCCTTCgcacgaaca                               >  1:206305/1‑141 (MQ=255)                                                                                             cAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGCTTTCAGGCGGGTGGCGCGGTGGCAAATCTCTTTAGCGAACGGCCTCCgagcgaaca                               >  2:498553/1‑136 (MQ=255)                                                                                              aGCTGACTGAACTGAGAGAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTGGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGaaaaa                              <  2:678081/143‑1 (MQ=255)                                                                                                          tggaaaaaTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCCTGGTGTTGCCGGTTTTCAGTCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCaaa                  <  1:58508/140‑1 (MQ=255)                                                                                                            aaagaaTATGCGTTATGCTGCAAAGAGACCAGCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaaga                <  2:561901/143‑1 (MQ=255)                                                                                                                        gTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCAc    >  1:542328/1‑143 (MQ=255)                                                                                                                         tATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACt   >  1:619045/1‑143 (MQ=255)                                                                                                                          aTTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACtt  <  1:465290/143‑1 (MQ=255)                                                                                                                                     |                                                                                                                                    GCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTT  >  NC_000913/3041500‑3041763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑