| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 759,250 | G→T | R182L (CGC→CTC) | sucA → | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 759,250 | 0 | G | T | 95.7% | 59.9 / NA | 24 | R182L (CGC→CTC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (13/9); total (14/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTC > NC_000913/759110‑759386 | ccAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAActct < 1:722962/143‑1 (MQ=255) aaCGTCGGTTCATGTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCg < 2:1460/143‑1 (MQ=255) tCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTc < 1:357569/143‑1 (MQ=255) ccATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACATACTGCGGCCCGACTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGCTCCAACAGCGTCTCGAGCCGGCTCGCCCCACTATCaccagc > 2:604350/1‑138 (MQ=255) aCTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAgaga > 1:563415/1‑143 (MQ=255) ctgctgGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGctt > 1:704608/1‑143 (MQ=255) tgGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAGCAGCGTATCGGGGCTGGTCGCGCGACTTGCAATAGCGAAGAGAGAAAACGCGGCTTa > 1:196605/1‑143 (MQ=255) tgGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTa < 1:53561/143‑1 (MQ=255) cTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACt > 1:35079/1‑143 (MQ=255) tCAAGCAAACCTACTGCGGCCCGATTGGTGCCGCGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAACCAACTg > 2:142104/1‑143 (MQ=255) tCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGATGAGAAAAAACGCTCCTGAAGCGAACTg > 2:586824/1‑143 (MQ=255) aaCCTCCTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAccgccg < 2:554438/143‑1 (MQ=255) atggCGGCCAGCTTGGTGCCGGGGATATGCGCATTACCAGCCCGGAAGAAAATGTCTGGATCCAACAGCGTATCGATTCTGTTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAATCGAACTGACCGCCGCGGaa < 2:518712/140‑1 (MQ=255) gCCAGATTGGGCCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGAGCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCtt < 1:472668/143‑1 (MQ=255) tGCCGAGTATATGCACATTACCAGCACCGAAGACAGGAACTAAAATCATCGGCGGATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTAcc > 1:146021/1‑143 (MQ=255) aGTATATGCACATGACCAGCACCGAAGCAAAACTCTGGCTCCAACAGCGTATCGAGTCTGGTCGCGCGCCTTTCCATAGCGAAGAGCGAATACGCGTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGCACGTTACCTCGgc > 2:628404/1‑143 (MQ=255) gatGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCaa < 2:563415/142‑1 (MQ=255) gCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATc > 1:60332/1‑46 (MQ=255) gCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATc < 2:60332/46‑1 (MQ=255) cacaTTACCAGCACCGAAGAAAAACTCTGGATCCAACAGc > 2:11776/1‑40 (MQ=255) cacaTTACCAGCACCGAAGAAAAACTCTGGATCCAACAGc < 1:11776/40‑1 (MQ=255) acaTTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTc > 1:709219/1‑143 (MQ=255) ccttcccAGCACCGAAGAAGAACTCTGGATCCCACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGCCCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTcc < 1:403701/138‑1 (MQ=255) aagaaAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAGAATTCCCTGGCGCAAAACGc > 1:569424/1‑143 (MQ=255) aaaaaCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTtc > 1:453185/1‑143 (MQ=255) | CCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTC > NC_000913/759110‑759386 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |