Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,196,220	C→T	H366H (CAC→CAT)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,220	0	C	T	96.0%	51.9 / NA	26	H366H (CAC→CAT)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (16/8);  minor base G (0/1);  total (17/9)
Fisher's exact test for biased strand distribution p-value = 3.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCGGTATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTC  >  NC_000913/1196080‑1196301                                                                                                                                             |                                                                                  accggtatcgCCCCCGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCCCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCatat                                                                                 <  1:374021/141‑1 (MQ=255)   cggtatcgCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATgg                                                                               >  1:567682/1‑143 (MQ=255)     gtatcgCCCCTGGTGCAAACATCGATGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACGGCGCCGAAATATGCCGGTCAGGACAACGTAAATCCTGGCGCTATGATTCACTCAGCTGAGATGATCCTGCGCcacaacagc                                                                             >  2:612584/1‑138 (MQ=255)       atcgCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCAACATTAAATTCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTg                                                                           <  1:557630/143‑1 (MQ=255)         cgCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGACATATGCCGGTCAGGCCCACGTAAATCCTGGCCCTATTATTCTCCCCGCTGAGATGCTGCTGCGCCACATggcgtcga                                                                         >  2:65420/1‑137 (MQ=255)          gCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCCCTATTATTCTCTCCGCTGAGATGATGCTACGCCATCTGGGCTGGAc                                                                        >  2:167021/1‑143 (MQ=255)                  tGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCgg                                                                >  1:531001/1‑143 (MQ=255)                      accctCGGTGAAGGATGGCCGCTGCTTGGAGCCAGCCGCGGTACTGCGCCGAAATATGCCTGCCAGGACAAAGGACATCCTGGCTCTGTCCTTCTCCCCGCTGTGATGATCCTGCGCCATATGGGTTGGACTGAAGCGGCTGa                                                            <  2:514157/139‑1 (MQ=255)                       aCATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAc                                                           <  1:669784/143‑1 (MQ=255)                            tgTGGCGAAGGCGCCCGGTTTGACGCAGCCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAAGCCTGGCTCTATTATGCTCTCCGCTGAGATGATGCTGCGCCAGATGGGTTGGACTGAAGCGGCTGAcctgat                                                      <  2:295438/142‑6 (MQ=37)                                    aggcgcCCGGTTCGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAg                                              <  1:7264/141‑1 (MQ=37)                                        gcCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGCAGCGGCTGCCCTGATTGTTACAGGTAt                                          >  1:85883/1‑143 (MQ=38)                                          ccTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGCTTGTTAAATGTATgg                                        >  2:115500/1‑143 (MQ=37)                                          ccTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATgg                                        >  1:423066/1‑143 (MQ=255)                                              tttGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAgg                                    >  1:446172/1‑143 (MQ=255)                                                tGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAGGCGGCTGACCTGATTGTTAAAGGTCTGGAAGgcg                                  >  1:24748/1‑143 (MQ=37)                                                   aGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCaa                               >  1:43807/1‑143 (MQ=255)                                                   aGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCaa                               >  1:467772/1‑143 (MQ=255)                                                     ccacccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGAGGATGCTGCGCCATCTGGGTTGGACTCACGCGGCTGACCTGCTTGTTAAAGGTATGGAAAGCGCAATc                             >  2:382729/1‑143 (MQ=255)                                                            cGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTAGCTCTATTATTCTCTCCGCTGAAATGATGCTGCGACATATGGGTTGGACTGAAGCGGCTGACCTGCTTGTGAAAGTGATGGGAGGAGCAATCAAtgcaa                      >  2:309651/1‑141 (MQ=21)                                                               tACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGGCCGGATTGTTAAAGCTATGGAAGGCGCAATCAATGCCaaga                   >  1:172073/1‑141 (MQ=21)                                                                aCTGCGCCGAAATAAGCCGGTCAGCACAAAGTGCCTCCCAGCGCTATTATTCTCTCCCCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGAc                  <  2:739927/143‑1 (MQ=14)                                                                  tGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGCTTGTTAAAGGTATGGAAGGCGCAATCCATGCCAAGACCg                >  1:53008/1‑143 (MQ=18)                                                                     gcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTaa             >  1:490322/1‑143 (MQ=18)                                                                      ccGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAAc            >  1:257935/1‑143 (MQ=18)                                                                       ggAAGTATGCCGGTCAAGACAGAGTACATCGTGGCTCTATTATTCTCTCCGCTGAGATGAGGCTGCGCCATATGGGTTGGCCTGCAGCCGCTGCCCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAAct           <  2:712338/142‑2 (MQ=14)                                                                          aacatGCCGGGCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGGTGGACTGAAGCGGCTGACCTGATTGTTAAAGGGATGGAAGGCGCAATCAATGCCAAGACCGTAACTTAt        <  1:167021/140‑1 (MQ=17)                                                                                ccGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCGCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTc  <  2:567682/143‑1 (MQ=14)                                                                                                                                             |                                                                                  ATCGGTATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTC  >  NC_000913/1196080‑1196301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑