Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,196,232	C→T	T370T (ACC→ACT)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,232	0	C	T	100.0%	49.8 / NA	22	T370T (ACC→ACT)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/8);  total (14/8)

TGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTC  >  NC_000913/1196097‑1196301                                                                                                                                        |                                                                      tGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCgg                                                                >  1:531001/1‑143 (MQ=255)     accctCGGTGAAGGATGGCCGCTGCTTGGAGCCAGCCGCGGTACTGCGCCGAAATATGCCTGCCAGGACAAAGGACATCCTGGCTCTGTCCTTCTCCCCGCTGTGATGATCCTGCGCCATATGGGTTGGACTGAAGCGGCTGa                                                            <  2:514157/139‑1 (MQ=255)      aCATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAc                                                           <  1:669784/143‑1 (MQ=255)           tgTGGCGAAGGCGCCCGGTTTGACGCAGCCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAAGCCTGGCTCTATTATGCTCTCCGCTGAGATGATGCTGCGCCAGATGGGTTGGACTGAAGCGGCTGAcctgat                                                      <  2:295438/142‑6 (MQ=37)                   aggcgcCCGGTTCGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAg                                              <  1:7264/141‑1 (MQ=37)                       gcCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGCAGCGGCTGCCCTGATTGTTACAGGTAt                                          >  1:85883/1‑143 (MQ=38)                         ccTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGCTTGTTAAATGTATgg                                        >  2:115500/1‑143 (MQ=37)                         ccTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATgg                                        >  1:423066/1‑143 (MQ=255)                             tttGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAgg                                    >  1:446172/1‑143 (MQ=255)                               tGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAGGCGGCTGACCTGATTGTTAAAGGTCTGGAAGgcg                                  >  1:24748/1‑143 (MQ=37)                                  aGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCaa                               >  1:43807/1‑143 (MQ=255)                                  aGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCaa                               >  1:467772/1‑143 (MQ=255)                                    ccacccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGAGGATGCTGCGCCATCTGGGTTGGACTCACGCGGCTGACCTGCTTGTTAAAGGTATGGAAAGCGCAATc                             >  2:382729/1‑143 (MQ=255)                                           cGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTAGCTCTATTATTCTCTCCGCTGAAATGATGCTGCGACATATGGGTTGGACTGAAGCGGCTGACCTGCTTGTGAAAGTGATGGGAGGAGCAATCAAtgcaa                      >  2:309651/1‑141 (MQ=21)                                              tACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGGCCGGATTGTTAAAGCTATGGAAGGCGCAATCAATGCCaaga                   >  1:172073/1‑141 (MQ=21)                                               aCTGCGCCGAAATAAGCCGGTCAGCACAAAGTGCCTCCCAGCGCTATTATTCTCTCCCCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGAc                  <  2:739927/143‑1 (MQ=14)                                                 tGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGCTTGTTAAAGGTATGGAAGGCGCAATCCATGCCAAGACCg                >  1:53008/1‑143 (MQ=18)                                                    gcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTaa             >  1:490322/1‑143 (MQ=18)                                                     ccGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAAc            >  1:257935/1‑143 (MQ=18)                                                      ggAAGTATGCCGGTCAAGACAGAGTACATCGTGGCTCTATTATTCTCTCCGCTGAGATGAGGCTGCGCCATATGGGTTGGCCTGCAGCCGCTGCCCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAAct           <  2:712338/142‑2 (MQ=14)                                                         aacatGCCGGGCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGGTGGACTGAAGCGGCTGACCTGATTGTTAAAGGGATGGAAGGCGCAATCAATGCCAAGACCGTAACTTAt        <  1:167021/140‑1 (MQ=17)                                                               ccGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCGCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTc  <  2:567682/143‑1 (MQ=14)                                                                                                                                        |                                                                      TGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTC  >  NC_000913/1196097‑1196301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑