| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,637,645:1 | +T | 100% | coding (4/1500 nt) | pitA → | metal phosphate:H(+) symporter PitA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,637,645 | 1 | . | T | 94.9% | 118.4 / ‑0.8 | 39 | coding (4/1500 nt) | pitA | metal phosphate:H(+) symporter PitA |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base T (16/21); total (17/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGC‑T‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCG > NC_000913/3637509‑3637780 | tataAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGC‑ttacat > 2:341472/1‑138 (MQ=255) taAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACAttt < 2:652813/143‑1 (MQ=255) gATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACAtttgtt < 1:160237/143‑1 (MQ=255) aaTTATTTTTTGAGTGAAATCAATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCACGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGCTATATACTT‑ACATTTGTTTGc > 2:428415/1‑143 (MQ=255) ttattTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGCCGTAACCTGCCATGCTT‑ACATCTGTTTGCTg > 2:190813/1‑143 (MQ=255) gtgtcaaaTCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTTACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGAtt < 1:502492/138‑1 (MQ=255) gAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGAtt < 1:505971/143‑1 (MQ=255) tGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACACTTGATAGATGGCCTGGATTTGc > 1:101963/1‑143 (MQ=255) tGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATAGTTCACTTTGCCCGCGCAGCGAAAGTCAATGATGATGCGCCGAGTTCATGTCCTCAAAATGCCGCAACGTCCTATTC‑TCACGTTTGTTTGCTGGCCTGGCTTTGc > 2:293488/1‑143 (MQ=255) aaaTCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCGCTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCAt > 2:618104/1‑143 (MQ=255) tCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATAcc > 2:217563/1‑143 (MQ=255) tCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGAGCAGCGAAAGTAACTGATAATGCGCCGCGTTCATGTCCTCAAAATGACGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTTCATAcc > 2:107756/1‑143 (MQ=255) tACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGc > 1:617120/1‑143 (MQ=255) ccAGGCGGCAGATCAAAAAAAGTCTCTATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCt < 2:303347/142‑1 (MQ=255) cAAACCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCCCCGGTAATGCGCGGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTtattat < 1:480726/143‑1 (MQ=255) tataTTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCttt > 1:249377/1‑143 (MQ=255) tatTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTtg < 1:454529/143‑1 (MQ=255) atTTCACTTTGCCGGCGCCGCGACAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAAGGGCGTAACGTCCTATGCTT‑ACATTTGTTTGGTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTtgt < 2:554104/143‑1 (MQ=255) tttCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAGAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCCTACCGGTCTGTTATTCTTGCTTGCACTGGCTTTtgtg > 2:548953/1‑143 (MQ=255) tttCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTtgtg < 1:617071/143‑1 (MQ=255) aCTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGt < 1:432772/143‑1 (MQ=255) tttGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACAGGTCTGTTATTATTGCTTGCACTGGGTTTTGTGcagaaa > 1:408098/1‑138 (MQ=255) gccgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGcc < 1:105904/143‑1 (MQ=255) cgcgAGAGTCACTGATAACGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCAt < 1:237644/143‑1 (MQ=255) gAATGTCACTGATCATGCGCCGCGTTCTGGTCCTCAAGATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCaa < 1:590869/143‑1 (MQ=255) gAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCaa < 1:569619/143‑1 (MQ=255) tCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCACTGGtt > 1:7595/1‑143 (MQ=255) cACTGATAATGCGCTGTGTTCATGGCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGttt < 2:492442/143‑1 (MQ=255) aCTGATAATGCGCCGCGTTCATGTCCTCAAAATGACGTAACGTCCTATACTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATGAATAGtctt > 2:141044/1‑140 (MQ=255) tgaTGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGa < 2:102491/141‑1 (MQ=255) tGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGacac > 2:660039/1‑143 (MQ=255) tGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTg > 2:71507/1‑143 (MQ=255) cTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCaa > 1:633199/1‑143 (MQ=255) cTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCaa < 1:540711/143‑1 (MQ=255) tCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTCTTGCTTGCACTGGCTTTTGTACTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAAc > 1:563951/1‑143 (MQ=255) aTGGCGTAACGTCCTATGC‑TGACATTTGTTTGCGGGCCTGGCTTTCCTTGACAGGCTGTTGTTCTTGCTTGCACGGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTa < 2:643700/143‑1 (MQ=255) tGGCGTGACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTAt < 2:97766/143‑1 (MQ=255) tGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTAt < 2:249377/143‑1 (MQ=255) tGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTAt < 1:217563/143‑1 (MQ=255) ccTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCcgcg < 1:618104/143‑1 (MQ=255) | TATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGC‑T‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCG > NC_000913/3637509‑3637780 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |