Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC JC	NC_000913	1,979,486	IS5 (+) +4 bp	100%	intergenic (‑271/‑264)	insA‑5 ← / → uspC	IS1 protein InsA/universal stress protein C

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1979486 =	0 (0.000)	40 (1.210)	33/264	NT	100%	intergenic (‑271/‑267)	insA‑5/uspC	IS1 protein InsA/universal stress protein C
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1979489	0 (0.000)	39 (1.180)	32/264	NT	100%	intergenic (‑274/‑264)	insA‑5/uspC	IS1 protein InsA/universal stress protein C
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

AATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1979627‑1979486 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCA  >  NC_000913/2101749‑2101890                                                                                                                                                                                                                                                                                              AATCACTCCCGGTGATCATATAATTTCAAATGTAAATATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGTCTTTCTTTGTATTTAATTAGG                                                                                                                                               >  2:87149/1‑143            GTGATCATATAATTTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAA                                                                                                                                    >  1:211168/1‑143             TGATCATATAATTTCAAATGTAATTATAAGACAATAGTGTAATGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGAGTGTTGCATTTCTTTGTATTTAATTGGGGAAGGTGCGAAT                                                                                                                                   >  2:91680/1‑143                        TTTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGGAA                                                                                                                        >  1:351388/1‑143                                    TTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCT                                                                                                            >  1:500977/1‑143                                      ATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTCTTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGA                                                                                                          <  1:631680/143‑1                                                    TACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATGTCAT                                                                                            >  1:160706/1‑143                                                      ctaATTCTCCGCATTAAACAAGTGGTTAATTTATGTAAACCATTTCATTAACATTAAGTTGATTGTTGCCTTGCTTTGTATTTAATTAGGGAAGGTGCTAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTT                                                                                          <  2:131407/140‑1                                                         ATTCGCCGCATTCAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTT                                                                                       <  1:10/143‑1                                                            CTCCGCATTAAATAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCAT                                                                                    <  2:153281/143‑1                                                            CTCCGCATTAAAGAAGTGGTTAATATGTGTATAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATACTTATCGGGTGTCTCAGTCATTTCATTTCTGCAT                                                                                    <  2:401849/143‑1                                                                      AACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCAGTTCTTTATGTTTGGGCCG                                                                          >  1:148661/1‑143                                                                         AAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATT                                                                       <  1:583558/143‑1                                                                         AAGTGGTTAATATATGTAAAACATTTCATTAACATAAGGGTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATT                                                                       >  2:542751/1‑143                                                                           GTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTT                                                                     >  1:419686/1‑143                                                                              GTTAATATATGTAAAACATTTCATTAACATTCAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGACGGTGCGAATAAGCAGGGCAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGCTTCTTTC                                                                  >  2:198433/1‑143                                                                              GTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTC                                                                  >  2:239462/1‑143                                                                                       TGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAca                                                         >  2:248847/1‑141                                                                                         TAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATG                                                       >  1:355777/1‑143                                                                                         TAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATG                                                       >  1:375215/1‑143                                                                                          AAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTCTGAGCCGATTTTTCCTCCCGTAAATGC                                                      >  2:319114/1‑143                                                                                           AAACATTTCATTAGCATTAAGTGGTTTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCC                                                     <  2:188559/143‑1                                                                                             ACATTTCAGTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACCCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTT                                                   <  2:54985/143‑1                                                                                                  TCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATC                                              >  2:273760/1‑143                                                                                                  TCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGCTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATC                                              <  1:426729/143‑1                                                                                                    ATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCTTGTTTGTGCCGATTTTTTCTCCCGTAAATGACTTGAATCAG                                            >  1:184721/1‑143                                                                                                          ATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATT                                      >  2:515404/1‑143                                                                                                              AGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGA                                  <  1:631511/143‑1                                                                                                               GTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAC                                 >  1:394694/1‑143                                                                                                                   ATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGTCTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTT                             >  1:363720/1‑143                                                                                                                    TTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTT                            <  2:211168/143‑1                                                                                                                      GTTGCCTTTCTTTGTATTTACATAGGGAAGGTGCGAATAAGCGGGGACATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGCGCCGATTTTTTCTCCCGTACATGCCTTGCGTCAACCTATTTCGACCGTTTCT                          >  2:143933/1‑143                                                                                                                          CCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGC                      <  1:556952/143‑1                                                                                                                                  tggtTTTAAGTAGTGTAGGTGTGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTTATTTCTTCATGTTTGAGCCTATTTTGTCTCCCGTAAATGCCTTGAATCAGCCTATTTATACCGTTTCTTCGCTATTTAAG              <  2:143950/139‑1                                                                                                                                  TGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTAAGCCGATATTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAG              >  1:365393/1‑143                                                                                                                                  TGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGGGTGAGCCGATTTTTTCTCCCGTAAATGCCTTGTGTGAGCCTGATTAGACCGTTTCTTCGTCAGTTAAG              >  2:537608/1‑143                                                                                                                                    TATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGC            <  1:43310/143‑1                                                                                                                                      TTTTATTAGGGAAGGTGCGAATAAGCGGGGCAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTACATGTGTGAGCCGACTATTTCTCCCGTAAATGCCTTGAATCAGCCTACATAGACCGTTTCGTCGCCATTTcaagagt          >  2:16747/1‑136                                                                                                                                      TTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTACTTAGACCGTTTCTTGCCCATTTAAGtagt          >  2:273013/1‑139                                                                                                                                        TAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGCGGCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTA        >  1:121439/1‑143                                                                                                                                         AATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAGTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTCAGGCGTTAT       >  1:414730/1‑143                                                                                                                                          ATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATC      >  2:630533/1‑143                                                                                                                                          ATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGGTTCTTCGCCATTTAAGGCGTTATC      >  1:520885/1‑143                                                                                                                                            TAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTATTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGACAATTTAGCCGGTTTCTTCGCCATTTAAGGAGTTATCCC    >  2:493499/1‑143                                                                                                                                             AGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCC   <  1:445144/143‑1                                                                                                                                              GGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCA  <  2:537770/143‑1                                                                                                                                                                                                                                                                                              AATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1979627‑1979486 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCA  >  NC_000913/2101749‑2101890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.