| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 125,387 | C→T | 100% | Q791* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,387 | 0 | C | T | 100.0% | 91.0 / ‑6.7 | 33 | Q791* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (13/19); total (14/19) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCC > NC_000913/125247‑125519 | agaTCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAg < 1:178768/143‑1 (MQ=255) ccTGGCGAGAGTTTACGGCGTAGGTTCGGGCGTTGAGAGCGTGACCGCCTTCCCCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGtga < 2:349546/143‑1 (MQ=255) gCGAAAGATTACGGCGTGGGTTCTGACGGTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGaa < 1:80445/143‑1 (MQ=255) aaaGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTAGAACATGCTGAACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAacga > 1:562880/1‑143 (MQ=255) aaGATTACGGCGTAGGTTCTGACGTTTATAGCGTGCCCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAacgac < 1:169795/143‑1 (MQ=255) ggTTGTGACGTTTAGAGCGTGAGCTCCTTCGCCGAGCGGGCGCGTGATGGTCAGGATTGTGAACGCTGGCACATGCTGCACCCGCTGGAACCTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGc < 2:173946/143‑1 (MQ=255) gTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCTTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCa < 2:374902/143‑1 (MQ=255) gaggTTTATACCGTGACCTCCTTCACCGAGCTGGCGCTTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTAc < 2:178115/140‑1 (MQ=255) gACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACACGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTAc < 2:434102/143‑1 (MQ=255) gTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGAATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGa > 2:79700/1‑143 (MQ=255) ataGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCCTCTACCGACtat > 1:544033/1‑143 (MQ=255) cctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTc > 2:49713/1‑143 (MQ=255) tCACCGAGCTGGCGCGGTATGGTTAGGGTTGTGAACGCTGGAACATTCTGCACCCGCTGGAAACTCCGCGCGGTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCt < 2:392938/143‑1 (MQ=255) cTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGt < 1:318239/143‑1 (MQ=255) ggCGCGGTATGGTGAGGATTGTGAACGCGGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTcc < 1:497401/143‑1 (MQ=255) ggCGCGGGATGGTCAGGATTGTGAACGCGGGAGCATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTcc < 2:365389/143‑1 (MQ=255) tGATGGTCAGCATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACtt < 1:349032/143‑1 (MQ=255) cgATGGTCAGGATTGTGAGCGCCGGAACATGCTGCACCCGCTGGAAACGCCGCGCGTTCCGTATATCGCGTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACtt < 2:111853/142‑1 (MQ=255) aGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATCTAGCGTAGGTGCTGAACCACGCTCCGGCAGTGGCATCTACCGACTATATGAAGCTGTTCGCTGAGCAGGTCCGTAGTTCCGTAGCg > 2:264225/1‑143 (MQ=255) gaccGCTGGAACATTCTGCACCCGCTGGAAATTCCGCGCGTTTCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATATACCGACGATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTgacga < 1:444865/140‑1 (MQ=255) ctgcACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGGTGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGCTCCGTACTTACGTAACGACTCACGACTACCACGAACTggg > 2:72283/1‑143 (MQ=255) cACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATTAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCCGACGACGACCGCGGACCGGGTAc > 2:345365/1‑143 (MQ=255) cccGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTg > 1:390747/1‑143 (MQ=255) ccGCTGGAAACTCGGCGCGTTTCGTATCTCTGTTATGTGATGAACGACTCTCCGGCAGTGGCATCTACCGATTATGTGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGa < 2:89173/143‑1 (MQ=255) ccGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTgc > 1:270984/1‑142 (MQ=255) ccGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGa > 1:6282/1‑143 (MQ=255) aCTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCgg < 1:867/143‑1 (MQ=255) aCTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACGGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCgg < 1:336942/143‑1 (MQ=255) gcgcgTTCCAAAAATAGCATAGGTGATGAACGACGATCCGGCAGTGGCATATACCGACTATATGAAACTGTTCGCTGAACAGGTCCGTACTTACGTACAGACTGAAGACGACCGCGCACTGGAGAATGATGGCTTCGGACGtt > 2:35686/1‑143 (MQ=255) cgctTTCCGTATATCGCTTAGTTGATGCACGACGCTCCGGCCGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTc < 2:370716/143‑1 (MQ=255) gcgTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTCCCGCGTACTCGGTACTGATGGCTTCGGTCGTTcc > 1:171323/1‑143 (MQ=255) ttCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCCGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGGACTGACGGCTTCGGTCGATCCggc > 2:184906/1‑141 (MQ=255) gTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAgac > 1:593993/1‑141 (MQ=255) | AGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCC > NC_000913/125247‑125519 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |