| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,661,969 | C→G | 100% | V55L (GTT→CTT) | iscR ← | DNA‑binding transcriptional dual regulator IscR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,661,969 | 0 | C | G | 99.3% | 107.4 / ‑7.0 | 36 | V55L (GTT→CTT) | iscR | DNA‑binding transcriptional dual regulator IscR |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base G (21/13); total (22/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCA > NC_000913/2661831‑2662105 | cgccCTGTCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGa < 2:400202/143‑1 (MQ=255) ggCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCAttt < 1:280396/143‑1 (MQ=255) ggCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCAttt < 2:622894/143‑1 (MQ=255) cAGCCGCCCTTACCCTGACAACGGGTTGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCAttttt < 2:298712/143‑1 (MQ=255) cTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGCAAGCAGACCATTTTTGCGCAgg > 1:598734/1‑142 (MQ=255) tttACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGCACGCGGGAAAGCAGACCATTTTTACGCAGAc > 2:522093/1‑143 (MQ=255) tGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGATCAACGTAAGCTGGAAAGCAGCGCATTTTTACGCAGACGGGacac > 2:348570/1‑140 (MQ=255) gggTCGCATCTACAGATTCGCCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAgttgt < 2:336699/143‑1 (MQ=255) cATCTACATATTCTTCAACTGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGa < 1:155098/143‑1 (MQ=255) cATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGa < 1:181453/143‑1 (MQ=255) aTCTACAGGTGCGGCACGGGCGCGAATTGCTTGGCCAGCGGCGAGGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCGGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGAt < 2:126347/143‑1 (MQ=255) cAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGaa < 2:477032/143‑1 (MQ=255) tCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGa < 1:422376/143‑1 (MQ=255) tCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGa > 1:627155/1‑143 (MQ=255) tCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAGCAGGGGGTCCAGATAAGGCAGGGa > 2:220877/1‑143 (MQ=255) cGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGaa > 1:143434/1‑143 (MQ=255) tAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGt > 1:421101/1‑143 (MQ=255) tAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCACTgcgat > 1:475860/1‑139 (MQ=255) aaTTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGCTAAGAAAGGGAAATTCCCTGACGtt > 2:472625/1‑143 (MQ=255) acgtctgcAACGGGGATGCTGCTGGCATACCTGCGTGTGAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATCTTTACGCATACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGa < 2:135450/136‑1 (MQ=255) ttCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCCTTTTTACGCAGCCGGGCAAACAGTTGTTCCAGATAAGACAGGGCACTTCCCTGACGTGCGGaaa > 2:534812/1‑143 (MQ=255) ttCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaaa < 2:228645/143‑1 (MQ=255) cGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCTGACGGGAAAACAGTTGTTCCAGATAAGAACGGGAAATTCCCTGACGTGCGGAAata > 2:337087/1‑143 (MQ=255) ggCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAACACAGACGTTCCAGATAAGAAAGGGAAATTACATGACGTTCGGAAATATCAGCCa > 2:93045/1‑143 (MQ=255) ctgctgGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATACCAGCCAACGGtcc > 1:385993/1‑141 (MQ=255) gCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCGGGAAAGCAGACCATTTTTACGCAGTCGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAACCAACGGTACCGGccc > 2:540253/1‑143 (MQ=255) cTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGCAATATCAGCCCACGGTACCGGGCCCGCt > 1:385401/1‑143 (MQ=255) cTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATCTTTACGCAGACGGGCAAGCGGTTGTTCCAGATAAGAAAGGGAAATTCCCTGCGGTTCGGCCATCTCCGCCCACGGACcccgccccgcctgccag > 2:137762/1‑136 (MQ=255) aCAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCCGGGttc > 2:4416/1‑142 (MQ=255) aCAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTAGTTCCAGATCAGAAAGGGAAATTCCCTGACGCTCGGAAATATCAGCCAACGGTACAGGGCGCGCTTCAGAGtta > 2:545306/1‑142 (MQ=255) cAGATAACCTCTGCTTGTTCCACGTTCGCTGGAATCCAGACTA‑TTTTACGCAGATAGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAg < 2:244895/143‑1 (MQ=255) ccGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGCAAACAGTTGTTCCAGATAAGAAAGGGAAAAAACCCAATATCCGGGAATATCAGCCCACGGCACCGAGCCCGCTTCAGAGTTGAGCGCAACGTc > 1:481454/1‑143 (MQ=255) ccGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGCTGAGCGCAACgac > 2:190177/1‑141 (MQ=255) gCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATACGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACgg < 1:59530/143‑1 (MQ=255) cTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCGAGCATTGCGGTCACGGc > 1:489753/1‑143 (MQ=255) tGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCa > 2:201401/1‑143 (MQ=255) | CGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCA > NC_000913/2661831‑2662105 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |