| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,041,632 | T→A | 100% | V107E (GTG→GAG) | ygfZ → | folate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,041,632 | 0 | T | A | 98.3% | 73.3 / ‑6.6 | 33 | V107E (GTG→GAG) | ygfZ | folate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (0/2); major base A (14/15); minor base C (1/1); total (15/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTT > NC_000913/3041493‑3041773 | gccCAGTGCGACGCCAAAGGTAAGAGGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGAc < 2:566014/143‑1 (MQ=255) ccATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCa > 2:341270/1‑143 (MQ=255) gCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATcggg > 1:324677/1‑141 (MQ=255) tgtgGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGtgtg < 1:396681/143‑1 (MQ=255) aGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTgctgct > 1:80180/1‑143 (MQ=255) tccggtgcgcgACGGCGAGGGCTTTGCATGGATTGAACGGCGCGGCGTGCGTGAACCGCAGCTGACTGAACTGAAGAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCg < 2:271928/136‑1 (MQ=255) cTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGCCTGAACTGAACAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGCGCGTGTGCCGCTTGgtgctccccg > 2:239392/1‑136 (MQ=255) gTTCCGCGACGGCGATGGCTTTGCATGGAGTGAACGGCGCAGCGTGCGGGAACCGCAGCTGACTGAACTGAAAAAATATGCGGGAATCTATAGAGCGAGCATCGCGACAGCACACGAGGACGTGCTGCCTGGCGTTGACggag > 2:413506/1‑141 (MQ=255) ccGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAGCCGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTCGGCGCTGCCGGtcctc > 2:8856/1‑139 (MQ=255) cgcgACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTACAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCCTGGCGTTGCCCGGTTTCa > 2:83877/1‑143 (MQ=255) cgcgACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGAGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCa > 1:175022/1‑143 (MQ=255) gATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGGGGGTGCTGCTTGGGGTGCGCGGTTTTGAGgcgcgcgc > 2:364366/1‑143 (MQ=255) acggCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATCTTCTTTGTTTTTGTTTTTGTTCTTCTCTCCACACCACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGcgcgcc < 1:306102/141‑1 (MQ=255) aTGGCTTTGCATGGATCGAACGGCGCCGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGCGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGcgcgcc < 1:491320/143‑1 (MQ=255) tttGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCt < 2:20270/143‑1 (MQ=255) ttGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCTGGCGcgcgccgcgccg > 1:278413/1‑141 (MQ=255) ttGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGGGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGCCTAAtctc > 1:328773/1‑143 (MQ=255) tGAACGGCGCAGCGTGCGTGAGCCGCAGCTGACGGAACTGCAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATctct < 2:463665/143‑1 (MQ=255) gAACGCCCCAGCGTCCGTGACCCGCAGCTGACTGAACTGAAAACATATGCGGTAGCCCCTAAAGAGACCACCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCtt < 2:467844/143‑1 (MQ=255) gtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCg < 1:292221/143‑1 (MQ=255) cGCAGCTGTCTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATGGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaagaa < 1:320063/143‑1 (MQ=255) tgaactgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGCACTGCCTTCGAAAGAAAAACAGGTAg > 2:17720/1‑143 (MQ=255) tgaaGAAGTATCCGGTATTCTCTAAAGAGACCATAGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCGGGTTTTCAGGCGCGCGCCGCGCTGGGAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCaaa < 2:297767/143‑1 (MQ=255) aaaaaaTATGCGGTATGCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTGTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaaga < 1:69093/143‑1 (MQ=255) aaaaaaGATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaaga < 1:635834/143‑1 (MQ=255) aacaggatGCGGGATTCGCGAAAGAGGCCAGCGCAGCAGACGACGAGCGTGTGCGGTTTGGTGTTGCCGGGTGTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaagaa < 2:599149/137‑1 (MQ=255) aaaaTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGACGGGTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaagaag > 1:415175/1‑143 (MQ=255) aTTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGCACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACtt < 1:339807/143‑1 (MQ=255) ttCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACctt > 2:503303/1‑143 (MQ=255) ctAAAGAGACCATCCCGCCAGTCTACTATCTTGTTCTGCTTGTTGTTTCCGTTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACATGTAGTCAAAGAAGGCGCGACCACTTTGCTa < 1:612646/143‑1 (MQ=255) aaaGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATg < 2:478182/143‑1 (MQ=255) aaaGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGACGCGACCACTTTGCTATg > 1:324760/1‑143 (MQ=255) gTGACCATCGGGCCAGACGACGAGCGCGTTTTGCTTGGTGTTGCCGGTTTTCAGGCTCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGTTATGGtt < 2:581870/143‑1 (MQ=255) | GCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTT > NC_000913/3041493‑3041773 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |