Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,657,601 G→C 10.0% intergenic (+34/+385) hdeD → / ← arrS acid‑resistance membrane protein/small regulatory RNA ArrS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,657,6010GC10.0% 101.0 / 5.6 41intergenic (+34/+385)hdeD/arrSacid‑resistance membrane protein/small regulatory RNA ArrS
Reads supporting (aligned to +/- strand):  ref base G (17/19);  new base C (2/2);  total (19/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.87e-01

AACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGG  >  NC_000913/3657459‑3657735
                                                                                                                                              |                                                                                                                                      
aaCGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGAGCATAGTCACTTg                                                                                                                                        >  1:113999/1‑143 (MQ=255)
 aCGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATTGTCACTTGa                                                                                                                                       >  1:203400/1‑143 (MQ=255)
         ggTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTa                                                                                                                               <  1:429693/143‑1 (MQ=255)
                   aCGCTGGTTTCCACACTGGTGCGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTATGATCCgggt                                                                                                                     <  1:451957/143‑4 (MQ=39)
                     gCTGGTTTCCACACTGGTGGGAATTGAGCTGGTATTTGGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTaa                                                                                                                   <  2:125310/143‑1 (MQ=255)
                      cTGGTTTCCGCTGTGTTGGGCATTGAACGGTTATTTGTCGCCGCCAGTCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCGCACGGATCATAGTCACTTGATGTGACTATGATCCGATTAAt                                                                                                                  <  2:313860/143‑1 (MQ=255)
                         gTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACACCGGTTGTCACCCGGCACATAGTCACTTGATGTGACTGTGATCCGATTAATAcc                                                                                                               >  2:432306/1‑142 (MQ=255)
                         gTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCAAGCCTGTTCAGCTTCGCCAGGTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTAACCCGGATCATAGTGGCTTGATGTGACTATGATGAGATTGATAct                                                                                                               >  1:60662/1‑143 (MQ=255)
                              cacaCTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACtctctc                                                                                                          >  1:166424/1‑143 (MQ=255)
                               acacTGGTGGGACTTGGACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGTCTATGATCCGATTAATACTCTCTcc                                                                                                         <  2:64953/143‑1 (MQ=255)
                                 acTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGc                                                                                                       >  2:198679/1‑143 (MQ=255)
                                      tggGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGc                                                                                                  >  1:215741/1‑143 (MQ=255)
                                       gggAATTGGACTGATGGTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCa                                                                                                 <  1:458501/143‑1 (MQ=255)
                                            ttGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTTACTATGATCCGATTAATACTCTCTCCGCTACGCAGcgtt                                                                                            >  1:618738/1‑143 (MQ=255)
                                               agctgggatTTGGCGCCGCCAGCCTGTGCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTa                                                                                         <  2:205188/136‑1 (MQ=255)
                                                   gATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTAACACGGTTCGCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATc                                                                                     <  1:305509/143‑1 (MQ=255)
                                                    atatTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATcg                                                                                    >  1:509974/1‑142 (MQ=255)
                                                       tttAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAAtt                                                                                 >  2:585142/1‑143 (MQ=255)
                                                        ttggcgcCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTg                                                                                <  1:388425/140‑1 (MQ=255)
                                                                        gTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGaaa                                                                <  1:263159/143‑1 (MQ=255)
                                                                               ttCGCCAGTTTATTCCTTAAGCAGCAATAATTACCCCGTTTGTCACCCTGATCATAGTCACTTGATGTGACTATTATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTAc                                                         <  2:657122/143‑1 (MQ=255)
                                                                               gtggCCGGTTTGTTCGTTACGTAGCAATAATTACCCCGGTTGTCACTCGGATCATAGTCATTTGATGTGACTATGATCCGATTAATCCTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTAc                                                         <  1:138676/140‑1 (MQ=255)
                                                                                  gCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGTTGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGAGCAATTGCGCACTATCATTGAAATAATTACCTg                                                      >  1:593747/1‑143 (MQ=255)
                                                                                  gCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCCGTGTTGTAGATCAATTGCGCACTATCATTTAAATCATCAcccg                                                      >  1:132831/1‑141 (MQ=255)
                                                                                   cctgTTTGTGCGGTACGCAGTGATAATTACCGCGGTTGTCACGCGGATCATAGTCACTTGATGTGACTATGATGCGATTAAGACGCTCTCCGCTACGCAGGGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGc                                                     <  2:213961/140‑1 (MQ=255)
                                                                                        ttGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGCCTATGATCAGCTCAAAACTGTTTCCGAAGTATAAGCGAGGGGAGCAATTGCGCACTATTATTGAAATAATTACCTGCTCGTg                                                >  2:139402/1‑143 (MQ=255)
                                                                                          gTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGat                                              <  1:398647/143‑1 (MQ=255)
                                                                                            tCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGatta                                            <  1:599961/143‑1 (MQ=255)
                                                                                              gTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAACTAATTACCCGCTACTGattatt                                          >  1:241792/1‑143 (MQ=255)
                                                                                               ttAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTCTCATTGAAATAATTACCTGCTAGCTAGTAttt                                         >  2:561698/1‑143 (MQ=255)
                                                                                               ttAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTCCCTGCTAGTTATGAttt                                         >  2:132999/1‑143 (MQ=255)
                                                                                                  agcagcAATACTTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCaa                                      <  2:360189/143‑1 (MQ=255)
                                                                                                    cagcaATAATTACCCCGGTGGTCACCCGGATCATAGGCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAAcc                                    <  1:14552/143‑1 (MQ=255)
                                                                                                         ataatTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGCTTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCCGCTCGTGATTATTTCAACCTACTg                               >  2:417653/1‑143 (MQ=255)
                                                                                                              tACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAAttt                          <  2:565035/143‑1 (MQ=255)
                                                                                                               gtcccGGTTGTCACCCGTATCATAGTCACTGTATGTGGCTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTc                         <  2:481859/141‑1 (MQ=255)
                                                                                                                         tCACCCGGAGCATAGGCACTTGAGTTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAAtttt               <  1:290610/143‑1 (MQ=255)
                                                                                                                          cACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAAttttt              <  1:198679/143‑1 (MQ=255)
                                                                                                                                 aTCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCActc       >  1:498208/1‑143 (MQ=255)
                                                                                                                                 aTCATAGTCACATCAAGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATAACACGCTAACGATTATTTCAACCCTCTCAGTTTCATCTACTTTTTTTCActc       >  1:90147/1‑143 (MQ=255)
                                                                                                                                    aTACTCATTTGTTTTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTAt    <  1:587659/143‑1 (MQ=255)
                                                                                                                                      aGTCACATCAAGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGGGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATgg  >  1:344645/1‑143 (MQ=255)
                                                                                                                                              |                                                                                                                                      
AACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGG  >  NC_000913/3657459‑3657735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑