| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 759,250 | G→T | 100% | R182L (CGC→CTC) | sucA → | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 759,250 | 0 | G | T | 100.0% | 84.9 / ‑6.7 | 34 | R182L (CGC→CTC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (17/15); total (19/15) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTC > NC_000913/759113‑759386 | ggAGACCTGCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGa < 2:202327/143‑1 (MQ=255) gaCCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATcc < 1:189225/143‑1 (MQ=255) ttCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACGGCGGCCCGATTGGTGCCGAGTATATGCACAATACAAGCACCGAAGAAAAAACCTGAACCCAACa > 2:77295/1‑143 (MQ=255) cAACGCCGGTGCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGc < 1:117467/143‑1 (MQ=255) aCGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGAGGCCCTATTGGTGCCGGGTATAGGCACATTACCCGCACCGAAGAGAGCCGCTGGCTCCAGCAGCgg > 2:317072/1‑142 (MQ=255) tCGGTTCACATGCCAGCGGCAAAGAAGCCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATc < 1:561286/143‑1 (MQ=255) gTTCATCTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAGCAGCGTATCGAg < 2:378649/143‑1 (MQ=255) tgctttGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTc < 1:23999/140‑1 (MQ=255) cAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCGCATTACCAGCACCGAAGAAAAACTCTGGATCCCACCGCGCGTCGTGTCTGGTCGGGCCACtg > 1:190143/1‑142 (MQ=255) cAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACCTTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACtt > 2:272938/1‑143 (MQ=255) tGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGaa < 1:544057/143‑1 (MQ=255) gctcccggAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCGAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGct < 1:342840/137‑1 (MQ=255) gctgGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGcttct < 1:243416/143‑1 (MQ=255) tgGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGCAGAGCAAAAACGCTGCTTa > 2:579796/1‑143 (MQ=255) ggAAGCCCACAAGCAAACCCACAGCGGCCCGATCGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTaa < 1:210893/143‑1 (MQ=255) gAAGCCCTCAGGCAAACCTACTGCGGCCCGATTCGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAg < 1:597005/143‑1 (MQ=255) aaGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAATAAACGCTTCTTAAGc < 1:533511/143‑1 (MQ=255) aaGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAACAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGc < 1:420239/143‑1 (MQ=255) aGCCCGCAAGCAATCCTACTGCGGCCCGATTGGTGCCGAGTATACGCACATTACCAGCACCGAAGAAAAACTCTGGATCCTACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCg < 2:610805/143‑1 (MQ=255) ccTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAAc > 1:474112/1‑143 (MQ=255) cTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGCGTCTGGTCGGGCGACTTTCAATAGAGAAGAGAAAAAACGCTTCATAAGCGaaat > 2:197697/1‑141 (MQ=255) aaaCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTGTGGATCCAACAGTGTATGGAGGCTGGTCGCGCGACTGTCAATAGCGAAGAGAGAAAACGCGTCTTGAGCGGACTGAccgcc > 1:202390/1‑143 (MQ=255) cTACTGCGGCCCGATTGGTGCCGAGTAGATGCACACTACCAGCACCGAAGAAAAACTCTGCATCCAACAGCGTAACCAGTCTGGTCGCGCGACTTTCAATAGCGACGAGAACACACTCTTCTTAAGCGCACTGACCGCCCCtc > 2:358646/1‑142 (MQ=255) tACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGGTCCAAGAGCGTCGCGAGTCTGGTCGCGCGGCTTTGCATGGAGAAGAGAAAAAACGCTGCTTAGGTGAACTGACCGCCGCTGa > 1:165565/1‑143 (MQ=255) tACTGCGGCCCGATTGGCGCAGAGAATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGCCTGGTCGCGCGACTTTCTATAGCGAACAGACAAAACGCTTCTTAAGCGAACTGACCGCCGCTGa > 2:504729/1‑143 (MQ=255) tGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAgg > 2:386710/1‑143 (MQ=255) ggCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCt > 2:373974/1‑143 (MQ=255) cGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGCGTCTGGTGGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGCACTGACCGCCGCTGAAGGTCGTgga > 2:246547/1‑141 (MQ=255) gATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAAc > 1:630039/1‑143 (MQ=255) cAGCACCGAAGAAACACTCTGGCTCCAACGGCGTGTCGGGTCTGGTCGCGCGACTTTCGCGAGGACGGATGAAAAACGCTTCTTGATCAAACTGACCTCCTCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgcg < 1:305655/143‑1 (MQ=255) aGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGccgc > 2:130228/1‑143 (MQ=255) cACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCaa > 1:411498/1‑143 (MQ=255) aagaaAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGGCTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGc > 2:530658/1‑143 (MQ=255) aaaaaCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGACTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAACACGCttg > 2:334278/1‑142 (MQ=255) | GGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTC > NC_000913/759113‑759386 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |