| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 940,751 | T→C | 10.1% | intergenic (+31/‑208) | serS → / → dmsA | serine‑‑tRNA ligase/dimethyl sulfoxide reductase subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 940,751 | 0 | T | C | 10.1% | 129.1 / 4.4 | 56 | intergenic (+31/‑208) | serS/dmsA | serine‑‑tRNA ligase/dimethyl sulfoxide reductase subunit A |
| Reads supporting (aligned to +/- strand): ref base T (25/25); new base C (4/2); total (29/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.42e-01 | |||||||||||
GTTGGTCGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCT > NC_000913/940610‑940892 | gTTGGTCGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTg > 2:218745/1‑143 (MQ=255) ttGGTCGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGc > 1:72918/1‑143 (MQ=255) gtGGGCGTACGCTGGGTGCAGTGATGGAATACTGTCAGCGGGCTGATGGTCGTAGTGAAGTACCAGAAGTTCTGCGTCCGTATGTGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCCGc < 2:250734/142‑1 (MQ=255) ggTCGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCGGATGGTCGTATTGAAGTACTAGATGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGTCTTTTACCCAATTTTTCTGAATCTAAAAAGCGCCTGCgg < 2:167793/143‑1 (MQ=255) gTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATCCCCACTTTTTCTGAATCTAAAAAGCGCCTGCGAgcgc > 1:2365/1‑143 (MQ=255) cTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATCGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATATCCAATTTTTCTGAATCTAAAAAGCGCCCGCAGGCGCttttt < 1:652100/143‑1 (MQ=255) tGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCtttttt > 2:301449/1‑143 (MQ=255) tAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCt > 1:462817/1‑143 (MQ=255) aagcgtaCTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTg < 2:442237/137‑1 (MQ=255) ggAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAACAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGa > 1:25264/1‑143 (MQ=255) ggAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGAATGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGa > 2:291469/1‑143 (MQ=255) gAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGGGCCTGAGGGCGCTTTTTTTGTCTCCCGTTGAt > 2:281809/1‑143 (MQ=255) aaaCTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATAc > 1:510453/1‑143 (MQ=255) cagGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACaataa > 2:526310/1‑143 (MQ=255) cagGCTGATCGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACaataa < 1:211576/143‑1 (MQ=255) tGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTAct < 2:183950/143‑1 (MQ=255) gATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATTTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTActc < 2:504438/143‑1 (MQ=255) tGGTCGTACTGAAGTACCAGAAGCTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTActcct < 2:659421/143‑1 (MQ=255) gTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCa < 1:194734/143‑1 (MQ=255) tCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAGTACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGGCTCCCTTTGATACCGAACAATAATTACGCCTCAc > 2:116255/1‑143 (MQ=255) ggaCCAGGAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTTCTCCTCACTTACACGTaa < 2:247450/141‑1 (MQ=255) aCCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAAta < 1:301449/143‑1 (MQ=255) cAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAAtact > 2:26034/1‑143 (MQ=255) aGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAAtacta < 1:2156/143‑1 (MQ=255) aaGTTCTGCGTCCGTATATGAACGTACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTTAATAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATactact < 1:588303/143‑1 (MQ=255) cGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACAACTCTCGAGTGaaac > 2:257448/1‑142 (MQ=255) tatGAACGGACTGGAATATATTGGCGAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTGTGTGTCTCCCCCTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTa < 1:484109/143‑1 (MQ=255) aggaccGGACTGGACAATATTGGCTAATACCCAATTTTTCTGAATCTCAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTAc < 1:281809/138‑1 (MQ=255) gACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCCGAGCGCGCTTTTTTTGTCTCCTTTTGATACCCAACAATAATTACTCCTCACTTACACGGAATACTACTTTCGAGTGAAAATCTGCCTAtcta > 1:659379/1‑142 (MQ=255) tGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCttt > 1:229552/1‑143 (MQ=255) tGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTATCTCCCTTTGATACCAAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTAACTATCTCttt > 2:7404/1‑143 (MQ=255) gAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAAAAATAATTACTCCTCACTTACACGTAATACTAATTTCGAGTGAAAGTCTGGCTATCTCTTTGa > 1:267070/1‑143 (MQ=255) ttGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCaa > 1:434887/1‑143 (MQ=255) tACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGGGAAAATCTACCGATCTCTTTGATTTTCAAATTAGTCg > 1:218284/1‑143 (MQ=255) cacAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAACTTATTCgtt > 1:307245/3‑141 (MQ=255) ccAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTACTACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATg > 2:111287/1‑143 (MQ=255) aaTTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGCACAATAATTACTCCTCACTTACACGTAATACTACTTACGAGTGAAAATCTACCTATCTCTTTGAATTCAAACTTATTCGATGta > 2:257510/1‑143 (MQ=255) aaTTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGta < 2:20494/143‑1 (MQ=255) ttttCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACATAACAATAATTACTCCTCACTTACACTTAATACTACTTTCGAGTGAAAATCTACCTATATCTTTGATTTTCAAATTATTCGATGTATAc > 2:534619/1‑143 (MQ=255) cTGACTCTAACAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCTCTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGc < 2:647615/143‑1 (MQ=255) gAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCt < 2:480311/143‑1 (MQ=255) gAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCt < 2:462817/143‑1 (MQ=255) caaaaaaaGCGCCCGCAGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGCTGTATGCAAGCCtata > 1:408513/4‑143 (MQ=255) cTAACAAGCGGCTGCGGTCGCTTTTTTTGTCTCCCTGTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCtatat < 1:617919/143‑1 (MQ=255) aaaaaGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAg > 2:299562/1‑143 (MQ=255) aaaaaGCGCCCGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATTTATACAAGCCTATATAg > 2:86927/1‑143 (MQ=255) aaaaaGCGCCCGCAGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAGTCTACCTATCTCTTTGATGTTCAAATTATTCGATGTATACAAGCGgatatgg > 1:1017/1‑141 (MQ=255) aaaaGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGc < 1:218745/143‑1 (MQ=255) aGCTCCTGCGGGCGCTTTTGTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATCGCGaa < 2:550057/143‑1 (MQ=255) aGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGaa < 2:434887/143‑1 (MQ=255) aGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGaa < 2:563699/143‑1 (MQ=255) aGCGCCTGCCGGCGCTTTTGTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGaa < 1:302225/143‑1 (MQ=255) aGCCCCTGCTGGTGCTCTTTCTGTCTCCCTTTGATACCGAACAATACCTACTCCTCACTTACACGTAATACTACTGTCGAGTGAAAGTCTACCTATCTCATTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGaa < 1:295588/143‑1 (MQ=255) gcgcCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAAc > 1:383278/1‑143 (MQ=255) ccTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACATATCTCTTTGATTTTCAAAGTATTCGATGTATACAAGGCTATATAGCGAACTGc > 1:44176/1‑143 (MQ=255) cTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAGCAATAATTAATCCTCCCTTACACGGAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCt < 1:656149/143‑1 (MQ=255) | GTTGGTCGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCT > NC_000913/940610‑940892 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |