| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,639,879 | (T)8→7 | intergenic (‑159/‑232) | uspB ← / → uspA | putative universal stress (ethanol tolerance) protein B/universal stress global stress response regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,639,872 | 0 | T | . | 97.1% | 141.0 / ‑1.9 | 35 | intergenic (‑152/‑239) | uspB/uspA | putative universal stress (ethanol tolerance) protein B/universal stress global stress response regulator |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base . (20/14); total (21/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.50e-01 | |||||||||||
CGGCGACCTGCCCGCGGAGTTCCACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTT > NC_000913/3639731‑3640003 | cgtcGACCTGCCCGCGGAGTTCCACCCCGGGGCTACCGCGCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGTAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGgttgtt < 2:611072/140‑1 (MQ=255)cGGCGACCTGCCCGCGGAGTTCCACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGgttgtt < 1:282347/143‑1 (MQ=255)cGGCGACCTGCCCGCGGAGTTCCACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGgttgtt > 1:466347/1‑143 (MQ=255)cGGCGACCTGCCCGCGGAGTTCCACCCCGGGGCTACCGCGCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGgttgtt < 1:683507/143‑1 (MQ=255) tGCCCGCGGAGTTCCACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGtttttttcca < 1:102695/143‑4 (MQ=255) cccGCGGAGTTCCACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCACGTCCTGGAGAGACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑GTTTTTTCCAGa > 1:16542/1‑143 (MQ=255) cgcgGAGTTCCACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGagg > 1:768329/1‑141 (MQ=255) gTTCCACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAAc < 1:481183/143‑1 (MQ=255) ttCCACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCCACATCCGAGCGGCTCTATGGATAGTGGAGGAGATCAGGTTG‑TCGTTTTCCCGACGGGTAAcc > 1:309134/1‑143 (MQ=255) aCCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTa < 1:735347/143‑1 (MQ=255) cccGGGGCTACCGCTCCCGATACGCTGCCAATCAGTGAACACCAGGTGCTGGAGAAACCGCTTTTGTGGTGACCAACAGACGAGCGGCTCTATAGATAGTGTAGGTGATCAGTTTG‑TTTTTTTCCAGAAGGTTAACCACTATc < 2:277937/143‑1 (MQ=255) ccGGGGCTACCGCTTCCGAGACGCGGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCa < 2:308351/143‑1 (MQ=255) ggggCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAat < 1:260005/143‑1 (MQ=255) cTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATGGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTGTCAGTATATTCATGt > 1:454275/1‑143 (MQ=255) gCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGttt < 1:731133/143‑1 (MQ=255) cAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTAt > 1:92626/1‑143 (MQ=255) aGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGTTCAACCACTATCAATGTATTCATTTCTAAAATTTGTTTTTCTCAc > 2:645938/1‑143 (MQ=255) tAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGCTCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGCCGAAAACTTGTTTGTCTAACgcg > 2:681098/1‑141 (MQ=255) aaCACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTGACGAGt > 2:294601/1‑143 (MQ=255) ccTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCg < 2:760676/143‑1 (MQ=255) ccTGGAGAAACCGCTTTTGGGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCg < 1:160213/143‑1 (MQ=255) cTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCgg > 2:305137/1‑143 (MQ=255) aaaCCGCTTTCGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGAc < 1:128059/143‑1 (MQ=255) aCCGCTTTTGTGGTGACCAACATACGAGCGGCTCTCTAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATTAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGTCCTATTGACgg > 2:117063/1‑143 (MQ=255) ttttGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGatcat > 1:443470/1‑143 (MQ=255) ggtgACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTT‑TTTTTTGTTAACCACTATCAATGTATTCATGTCATAACTTTGTTTCTCGAACGAGTACGCATGTCTTTCTGACGCATCATCCGGGt > 2:429866/1‑143 (MQ=255) tgACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCg < 1:473062/143‑1 (MQ=255) cAACATACGAGCGGCTCTATCGCTAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTCGTTTATCTCACGAGTACGCAAGGCGCATTGACCGATCATCCGCGTCGCtat > 2:112121/1‑143 (MQ=255) cAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGCATCATCCGGGTCGCtat > 2:716363/1‑143 (MQ=255) cGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTAtaaggtaa > 2:169757/1‑143 (MQ=255) ggCTCTAGAGATAGTGTAGGAGAGCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATAAATAGATGCATCTCGACAATCTGTTTATCTAACGAGTAAGGAATGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATg < 2:361178/143‑1 (MQ=255) ctctATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGCTCATCCGGGTCGCTATAAGGTAAGGACGGt > 1:405430/1‑143 (MQ=255) tctATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTGCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCAGCCGGGTCGCTATAAGGTAAGGATGGTc < 2:820746/143‑1 (MQ=255) agatagTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTAGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGGCGGTATAAGCTAAGGATGGTCTTAac > 2:436447/1‑143 (MQ=255) atagTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAacac > 1:648747/1‑143 (MQ=255) gtAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGCAAGCAAGGCGTATTGACTGCTCATCCGGTTCGCTATAAGGTGAGGATGTTCTTAACACTAAAt > 2:643181/1‑143 (MQ=255) tAGGCGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTGATGTCGAAAATTTGTTTCTCTAAGGAGTAAGCAAGGCGGATTGACGGATCATCCGGATCGCTATAAGGTAAGGATGGTATTGACACTGAATc > 1:35799/1‑143 (MQ=255) tAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATc > 1:446123/1‑143 (MQ=255) aGGAGAGCAGGGTA‑TTTGTTGCCAGACGGTTAACCACGATTAATATATGCATGTCAAAAAATTGTTCATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCt < 2:491124/143‑1 (MQ=255) cagaCCAGGTTC‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACTAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCttt < 1:337317/142‑1 (MQ=255) | CGGCGACCTGCCCGCGGAGTTCCACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTT > NC_000913/3639731‑3640003 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |