| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 125,387 | C→T | Q791* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,387 | 0 | C | T | 94.1% | 121.6 / ‑5.8 | 51 | Q791* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (2/1); new base T (20/28); total (22/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTAT‑ATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGA > NC_000913/125249‑125523 | aTCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGACACTCCGCGCGCTCCGTCT‑ATCGCTTAGGt > 2:318228/1‑143 (MQ=255) cGAAAGTTTACGGCGTAGGTTCTGTCGTTTATAGCGTGACTTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTAT‑ATCGCTTAGGTGATGAac < 2:30009/143‑1 (MQ=255) cGAAAGAGTGCGGCGTAGGTTCTGACGTTTATAGCGTCACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTAT‑ATCGCTTAGGTGATGAac < 1:724483/143‑1 (MQ=255) agcgtACGGCGTAGGTGCTGACGTTTATAGCGTGACCTCCTGCACCGAGCGGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGTTGGAAACTCCGCGCGTTCCGTAT‑ATCGCTTAGGTGATGAacgacg < 2:569408/139‑1 (MQ=255) ggCGTGGGTTCTGACGTTTATAGCGTGACCTCCTGCACCGAGCTGGCGCGTGAGGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTAT‑ATCGCTTAGGTGATGAACGACGCTCCGGc < 2:178166/143‑1 (MQ=255) tAGGTTCTGACGTTTATAGCGTGCCCTACTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTAT‑ATCGCTTAGGTGATGAACGACGCTCCGGCAGTg < 2:446563/143‑1 (MQ=255) ggggTTCTGACGTTTATCGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGCATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTAT‑ATCGCTTATGTGATGAACGACGCTCCGGCAGTg < 2:72382/141‑1 (MQ=255) tttATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTAT‑ATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGAc > 1:894475/1‑143 (MQ=255) tataGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTAT‑ATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACtg > 1:903174/1‑142 (MQ=255) tataGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATGGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTGT‑ATCGCTTACGTGATGAACGACGCTCCGGCCGTGGCATCTGCCGCCta > 1:229021/1‑143 (MQ=255) tataGCGCGGCCCCCTGCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTAT‑ATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACta < 2:804352/143‑1 (MQ=255) taGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTAT‑ATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACtata < 1:351795/143‑1 (MQ=255) aGCTTGACCTCCTTCACCGAGCTGGCGCGTGCTGCTCAGGATTGTGAACGCTGGAACATGCTGCAACCGCTTGAAACTCCCCGCGGGCCGTAT‑ATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTatat < 1:661470/143‑1 (MQ=255) cGTGACCTGCGTCACCGATCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTAT‑ATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGa < 2:202404/143‑1 (MQ=255) tcctTCACCGAGCTGGCGGGTGATGGTCAGGATTGTGAACGCTGTAACATGGTGCACCCGCTGGAAACTCCGCGCGTTCCGTAT‑ATCGCTTAGGGGATGAACGACGCTCCGGACGCGGGCTATACCGACACTGTGAAACTggt > 2:570844/1‑141 (MQ=255) tcctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAGCTGCGGGCGTTCCCCATCAACACTCCACT‑TGGTTCGTCGCTCCGGCAGTGGCATCTACCGACTAGATGAAACCGtt > 2:190208/1‑143 (MQ=255) tcctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTAT‑ATCGCTTAGGTGATGAACGACACTCCGGCAGTGGCATCTACCGACTATATGAAACTGtt > 2:632773/1‑143 (MQ=255) ctccgccGGGCTGGTGCGTGATGCTCAGCACTCTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTACCGTCT‑ATCGCGTAGGTGATGAACGACGCTCCGGCAGGGGCATCTACCGACTATATGAAACTGTTCg < 2:331295/138‑1 (MQ=255) ctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGTAACTCCGCGCGTTCCGTAT‑ATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCg < 1:454260/143‑1 (MQ=255) ctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTAT‑ATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTAGATGAAACTGGTCg > 1:802814/1‑143 (MQ=255) ggcggcgtGGTCAGGGTTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTAT‑ATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGt < 2:667910/136‑1 (MQ=255) gcggtacggTCAGGAGTGTGAGGGCTGCAACATGCTGCACCCGCTGGAAACTCCGCGCGTTGCGTAT‑ATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTa < 1:268757/136‑1 (MQ=255) gcgTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTGCCGTAT‑AGCGCGTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTGGGCGGGGCAGGTCCGTa > 1:559677/1‑143 (MQ=255) gcgGGATGGTCGGGAGTGTGAACGCTGGAACCTGCTGCACCCTCGGGAAACTCCGCGCGTTCCGGAT‑ATCGCTTGGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTa < 2:121408/143‑1 (MQ=255) gATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTAT‑ATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGc > 1:84207/1‑143 (MQ=255) gATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTAT‑ATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGGACTTGCGTACCGGc > 1:858173/1‑143 (MQ=255) tgtgAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTAT‑ATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGCGCAGGTCCGTACTTACGTACCGGCTgg > 2:605374/1‑142 (MQ=255) tgtgAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTAT‑ATCGCGCAGGTGATGAACGACGCTCCGCCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGCGCAGGTCCGTACTTACGTACCGGCTga > 2:836519/1‑143 (MQ=255) gtgAACGCTGTAACATGCTGCACCCGGTGGAAACTCCGCGCGTTCCGTAT‑ATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTgac < 2:712149/143‑1 (MQ=255) gtgAACGCTGGAGCATGCTGCACCCGCGGGGGGCGGAGCCCCTCCCGTAT‑ATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTgac < 1:376174/143‑1 (MQ=255) tgAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTAT‑ATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTCCTTACGTACCAGCTgacg > 2:649839/1‑143 (MQ=255) gagcgcgGGCACATGCTGCACCCGCGGGAAACGCAGCGCGTGCCGTAT‑ATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTgacga < 1:16412/140‑1 (MQ=255) aaCGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTAT‑ATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTgacgac > 1:312932/1‑143 (MQ=255) tGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTAT‑ATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCGGGTCCGTGCTTGCGTACCGGCTGCCGACGACcg > 1:685670/1‑143 (MQ=255) ggAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTAT‑ATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAGCTGTTCGCTGAGCAGGTCCGTACTTACGTACAGACTAACGGCTACcgc > 2:872005/1‑143 (MQ=255) aaCATGCTGCCCCCGCTGGAAACTCCGCGCGTTCCAGAC‑ATCTCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGt < 1:375767/143‑1 (MQ=255) cctcTGGAACCTCCGCGCGTGCGGTAT‑ATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGa < 2:766739/140‑1 (MQ=255) ccGCTGGAAACTCCGCGCGTTCCGTAT‑ATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGCTCCGTACTCACGTACCGGCTGACGACTACCGCGTACTGGGTACTGa > 2:614058/1‑143 (MQ=255) ccGCTGGAAACTCCGCGCGTTCCGTAT‑AGCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATAGGAAACTGGTCGCTGAGCAGGTGCGTCATTACGTACCGGCTGAAGACTACCGCGTACTGGGTACTGa > 2:614054/1‑143 (MQ=255) cGCTGGAAACTCCGCGCGTTCCGTAT‑ATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAACCTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGAt < 1:488661/143‑1 (MQ=255) tGGAAACTCCGCGCGTTCCGTGT‑ATCGATTCGGTGAGGAACGACGCTCCGGCAGTGGGATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGc < 1:700301/143‑1 (MQ=255) tGGAAACTCCGCGCGTTCCGTAT‑ATCGCTTAGGTGAGGAACGACGCTCCGGCAGTGGCATCTCCCGACTATATGAAACTGTTCGCTGAGCAGGTAGGTACTTACGTACCGGCTAGCGACCACCGCGCAATGGCTACTGATgac > 2:668811/1‑141 (MQ=255) gAAACTCCGCGCGTTCCGTAT‑ATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGAt < 2:375936/138‑1 (MQ=255) gAAACTCCGCGCGTTCCGTAT‑ATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGAt > 1:375936/1‑138 (MQ=255) cgcgTTCCGTAT‑ATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTc < 1:435361/143‑1 (MQ=255) gcgTTCCGTAT‑ATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTTGGTACTGATGGCTTCCGTCgaccc > 2:369935/1‑139 (MQ=255) tatctgTAT‑ATCACTTAGTTGATAAACGACGCTCCTGCAGTGGCTTTTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGa < 2:241265/138‑1 (MQ=255) gTTCCGTAT‑ATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGa < 1:499339/143‑1 (MQ=255) cggttt‑atCGCTTAGGTGATGAACGTCGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAg < 2:431846/138‑1 (MQ=255) atCGCTTAGGTGATGACCGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTga < 1:315754/143‑1 (MQ=255) atCGCTTAGGTGAGGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTga < 1:28119/143‑1 (MQ=255) | ATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTAT‑ATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGA > NC_000913/125249‑125523 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |