Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,661,969 C→G V55L (GTT→CTT)  iscR ← DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,661,9690CG97.7% 129.7 / NA 44V55L (GTT→CTT) iscRDNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (30/13);  minor base A (1/0);  total (31/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAG  >  NC_000913/2661830‑2662108
                                                                                                                                           |                                                                                                                                           
ccgccCCCGCAGCCGCCTTTACCCTGACAACGGTTGGCATCTACAGATGCGTCACCGGCGCTCTCTACTTCGCCAACGGCGATGCTGCTTGCACCTTTGCCTAACACATACCCACCGCCTGGTCCACGTACGCTGGAAAGCAg                                                                                                                                          <  1:124056/143‑1 (MQ=255)
                cTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACATCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCCGGTCCACGTCCGCTGGAAGGCAGAACATTTGTCCGAAGa                                                                                                                          >  1:264361/1‑143 (MQ=255)
                   tACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCACGGTCGATGCTGCTGGCGTCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCCGACCCTTTGTACGCAGTCgg                                                                                                                       >  1:678933/1‑143 (MQ=255)
                      ccTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTCCGCTGGAAAGCAGACCATTGTTACGCAGACgggca                                                                                                                    >  1:313931/1‑141 (MQ=255)
                      ccTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTCCTACGCACACGGGaa                                                                                                                    >  2:836850/1‑143 (MQ=255)
                       cTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGACGATACTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGCCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGaaa                                                                                                                   >  2:48861/1‑143 (MQ=255)
                        tGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGCCGGGacac                                                                                                                  >  2:133002/1‑140 (MQ=255)
                         gACAACGGGTGGCATCTACAGATTTGTCAACGGCGCTAAATACTTCGGCAACGGCGATGCCCCTGGCATCTGTGCCTAACAGATAACCACCGACTGGTCCACGTACGCTGGAAAGCAGACCGTCTGTACGCAGACGAGAacac                                                                                                                 >  2:121184/1‑143 (MQ=255)
                            aaCGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTTGACGTACGCTGGGAAGCAGAGCATGTTTACGGAGACGGGAAAACAgc                                                                                                              >  1:357504/1‑142 (MQ=255)
                                 gtcgCATCTACATATTCGTCAACGGCGCTAATTACTTCGCCAACTGCGATGCTGCTGGCAGCTTTGCCTAACAGACAACCAACGCATGGTTCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTc                                                                                                         <  1:83778/140‑1 (MQ=255)
                                     cATCTACAGATCCGTCAACGGCGCTAATTACTTCGCCAACGGCGACGCTGCTCGCATCTTTGCCTAACAGATAACCACCGCCTGGCCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGa                                                                                                     <  1:133002/143‑1 (MQ=255)
                                       tCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTAGTTCCAGATa                                                                                                   >  2:658480/1‑143 (MQ=255)
                                                   tCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAAt                                                                                       >  1:785912/1‑143 (MQ=255)
                                                             tAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGa                                                                                          <  2:274874/130‑1 (MQ=255)
                                                             tAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGa                                                                                          >  1:274874/1‑130 (MQ=255)
                                                             tAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGa                                                                                          >  1:747083/1‑130 (MQ=255)
                                                             tAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGa                                                                                          <  2:747083/130‑1 (MQ=255)
                                                                ttACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCg                                                                          <  1:320853/143‑1 (MQ=255)
                                                                gtACGTCGCCGACGGGGAGGCTGCTAGCATCTTTGCCGAACAGATAACCGCCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCg                                                                          <  2:596866/142‑1 (MQ=255)
                                                                    ttCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGGTGGAAAACAGACCATTTTTACGCAGACGGGAAGACAGTTGTGCCAGACAAGAAAGGGAGATTTCCTAACGTTCGGaaa                                                                      >  2:134436/1‑143 (MQ=255)
                                                                    ttCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAACGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAACTTCCCTGACGTTCGGaaa                                                                      >  1:161614/1‑143 (MQ=255)
                                                                         cAACGGCGATGCTGCTGGCATGTGGGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCa                                                                 <  1:861777/143‑1 (MQ=255)
                                                                         cAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTCCGATGTAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCa                                                                 <  1:898051/143‑1 (MQ=255)
                                                                         cAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCa                                                                 >  2:858346/1‑143 (MQ=255)
                                                                            cGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCTGGATCAGAAACGGAAACTCCCTGACGTTCGGAAATATCAGcc                                                              >  2:875097/1‑143 (MQ=255)
                                                                                        cggCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCggg                                                  <  1:869737/142‑1 (MQ=255)
                                                                                         ggcagtgttGCCTAGCAGAGAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGc                                                 <  2:532847/136‑1 (MQ=255)
                                                                                           cATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGccc                                               >  2:151477/1‑143 (MQ=255)
                                                                                            aTCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGACATATCAGACAACGGTACCGGGCCCg                                              >  2:846355/1‑143 (MQ=255)
                                                                                               tttGCCTAACAGATAACCACGGCCTGGTCCACTTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTCCCGGGCCCGcct                                           >  2:471868/1‑141 (MQ=255)
                                                                                               tttGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCATACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCtt                                           <  1:453787/143‑1 (MQ=255)
                                                                                                    cTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGCCGTTCGGAAACATCAGCCAACGGTACCGGGCCCCCTCCagag                                      >  2:779707/1‑143 (MQ=255)
                                                                                                     tAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGGCGCCAGTTGTTCCAGATAAGAAAGCGACATTCCCTGACGTTCGGCAATATCAACCCACGGTCCCGGGCCCGCTGCAGAGt                                     >  1:174452/1‑143 (MQ=255)
                                                                                                     tAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGAAAAACAGTTATCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGt                                     >  1:152986/1‑143 (MQ=255)
                                                                                                      aaCAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGtt                                    >  1:545938/1‑143 (MQ=255)
                                                                                                             aaCCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCa                             >  1:354591/1‑143 (MQ=255)
                                                                                                                    gCCTGGGGCACGTACGCTGGAAAGCCGACCAGTTTTACGGAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTGGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCaa                      <  2:534177/143‑1 (MQ=255)
                                                                                                                     ccTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAg                     >  2:424608/1‑143 (MQ=255)
                                                                                                                             aCGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGCGTTGAGCGCAACCTCCAGCATTGCgg             >  2:611694/1‑143 (MQ=255)
                                                                                                                                   gCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGGGTTGAGCGCAACGTCAAGCATTGCGGTCACgg       >  1:682690/1‑143 (MQ=255)
                                                                                                                                     tGGAAAGCAGACCATTTTTACGCAGACGGGCAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCa     >  1:640532/1‑143 (MQ=255)
                                                                                                                                       gAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATa   >  2:600890/1‑143 (MQ=255)
                                                                                                                                       gAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATa   >  1:545199/1‑143 (MQ=255)
                                                                                                                                        aaaGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGCAAATATCAGCCAACGGTACCGGGCCCGCTTCCGAGATGAGCGCAACGTCAAGCATTACGTACACGGCATaa  >  2:225502/1‑142 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAG  >  NC_000913/2661830‑2662108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑