Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,470	C→T	noncoding (35/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,470	0	C	T	96.1%	139.8 / NA	51	noncoding (35/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (24/25);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGC  >  NC_000913/696330‑696607                                                                                                                                             |                                                                                                                                          cGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAg                                                                                                                                         <  2:845121/143‑1 (MQ=255)       aTCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCg                                                                                                                                   <  2:91840/143‑1 (MQ=255)         ccGACATGGAAGGGTAAGACTAGTTAACTGCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGt                                                                                                                                 <  2:673043/143‑1 (MQ=37)          cGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAGTCCGGTg                                                                                                                                >  1:131771/1‑143 (MQ=255)          cGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTg                                                                                                                                >  1:899368/1‑143 (MQ=255)           gACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGGCGATGCGAGCGTTTTTAAGGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGCATTAGAATCCGgcgc                                                                                                                               >  2:734523/1‑143 (MQ=38)             cATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCt                                                                                                                             >  1:544023/1‑143 (MQ=255)               gtGAAGGATAAGACGCGCCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTa                                                                                                                           <  1:740943/142‑1 (MQ=255)                tGAAGGATACGACGCGTCAACATCGCATTCGACATTGCATGAACGCAAAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTAc                                                                                                                          <  2:216546/143‑1 (MQ=37)                tGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTAc                                                                                                                          >  2:765012/1‑143 (MQ=255)                  aaGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGGATCCGGTGCCTTACCg                                                                                                                        >  1:341212/1‑143 (MQ=255)                  aaGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCg                                                                                                                                   <  2:652550/132‑1 (MQ=255)                  aaGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCg                                                                                                                                   >  1:652550/1‑132 (MQ=255)                          gACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAATAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGCATCAGAATCCGGTGCCTTACCGCTTGGCGa                                                                                                                >  1:862877/1‑143 (MQ=255)                           aCGCGTCAACTTCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGATTCCGGTGCCTTACCGCTTGGCGAt                                                                                                               <  2:679425/143‑1 (MQ=255)                            cGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATa                                                                                                              <  1:348477/143‑1 (MQ=255)                             gtgtCCACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGACGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACATCGGAACGCCGGACTTAGACTCCGGCGCCGTACCGCTTGGCGATAc                                                                                                             >  2:620963/1‑143 (MQ=37)                                  aaCATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCaa                                                                                                        >  1:59640/1‑143 (MQ=255)                                  aaCATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCaa                                                                                                        >  1:344549/1‑143 (MQ=255)                                        gCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGt                                                                                                  <  2:810641/143‑1 (MQ=255)                                         cATTCGACATTGCATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGGTg                                                                                                 >  1:368816/1‑143 (MQ=255)                                         cATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTg                                                                                                 >  1:522928/1‑143 (MQ=255)                                          aTTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGc                                                                                                >  1:588440/1‑143 (MQ=255)                                           tttgACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCa                                                                                               <  1:746772/140‑1 (MQ=255)                                            tCGACATCGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTGTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAc                                                                                              <  1:592110/143‑1 (MQ=255)                                               aCATTGAATGAACGCAGGAGATCAAAAAGCCCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTAGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGAGACCCCAACGGGGTGCACTTa                                                                                           >  2:230461/1‑143 (MQ=21)                                                  ttGAATGAACGCAGAACAGCAATAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACaa                                                                                        <  2:468314/143‑1 (MQ=255)                                                      atgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTCGACCACGGTGCCTTACCTCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTa                                                                                    <  1:172203/143‑1 (MQ=37)                                                              atatacgCAAAAAGCTCGCCTAAGCGTGCTTTTTTAATTTGGCTGGTGTACGACGATTCGAACCTCGGCATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCtt                                                                            <  2:556837/137‑1 (MQ=21)                                                              aGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCtt                                                                            <  1:530922/143‑1 (MQ=255)                                                                  aaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCAGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGGTGGACTTCCGAGGGAAGCGTCTAGAAt                                                                        >  2:534706/1‑143 (MQ=21)                                                                     cAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACGAGCTCACCGCCTTGAAtcag                                                                     >  1:680445/1‑140 (MQ=21)                                                                      aaaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAt                                                                    <  2:816476/143‑1 (MQ=255)                                                                       aaaaGCTCGCCGAAGCGTGCTTTTTTAATGGGGCTGTGGTACGAGCATTCGAACCTCGGAATGCCGGAATTAGAACCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACTAGGTAAGCGTCTTGAATAAAtt                                                                   <  1:446779/143‑1 (MQ=25)                                                                       aaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATaaact                                                                   >  1:609290/1‑141 (MQ=255)                                                                         aggcgcgcgGAAGCGAGCTTTTTTAATGGGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTgg                                                                 <  2:735463/138‑1 (MQ=25)                                                                             tCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTgg                                                             >  1:748815/1‑143 (MQ=255)                                                                              cggcgtcgCGAGCTTTGTTAGTGGTGCTGGCGTAGCAGGTGTCGAACCTCGGAATGCCGGAATTAGAATTCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCTTCTTGAATAAAGTGGCTggg                                                            <  2:233241/136‑1 (MQ=18)                                                                              cggcGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGACACGCGGAAGGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTggg                                                            <  1:855491/140‑1 (MQ=25)                                                                                 cGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTGCAAGGTCCGCGTCTTGACTATATTGGCTGGGGTa                                                         >  2:315930/1‑143 (MQ=21)                                                                                  gAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGGACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGAGGTAc                                                        >  1:129162/1‑143 (MQ=255)                                                                                    aGCGAGCTTTTTTAACGTGGCTGGGGTACTAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGa                                                      <  2:494151/143‑1 (MQ=255)                                                                                                tAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTAAAAGGTAAGCGTCTGGAATAAATTGGCTGGGGTACGAGGATTCGAAcgt                                          >  1:225159/1‑141 (MQ=255)                                                                                                 aaTGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTc                                         <  1:634796/143‑1 (MQ=255)                                                                                                         tGGGGTACGAGGATGCGAACCTCGGAATGCCGGAATTAGAAGCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGcc                                 <  1:386679/143‑1 (MQ=255)                                                                                                                cGAGGATTCGAACCTCGGAATGCCGGAATTCGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCa                          <  1:374684/143‑1 (MQ=255)                                                                                                                 gAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTAACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGGACAGAGGAATGCCGGAATCAg                         >  1:873998/1‑143 (MQ=25)                                                                                                                  aGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGa                        <  1:671302/143‑1 (MQ=255)                                                                                                                    gactCGACCCTCGGCATGCCGGAAGTAGAATCCGGTGCCTTACCGCTTGGCGATACGCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAt                      <  2:588440/140‑1 (MQ=38)                                                                                                                          aaCCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACACGGTCCGCGCCTCGAATACATCTGCTGGGGTACGAGGCTACGAACCTCGGACCGCCGGAATCAGAATCCggag                >  2:228107/1‑141 (MQ=21)                                                                                                                                        ggAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgtc  >  1:161755/1‑141 (MQ=255)                                                                                                                                             |                                                                                                                                          CGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGC  >  NC_000913/696330‑696607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑