| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 759,250 | G→T | R182L (CGC→CTC) | sucA → | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 759,250 | 0 | G | T | 93.5% | 117.4 / NA | 46 | R182L (CGC→CTC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base T (24/19); total (25/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTC > NC_000913/759110‑759388 | ccAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAActct < 2:529008/143‑1 (MQ=255) caagagaCCTTCAACGTCGGTGCATCTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATAGGGGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTg < 2:724675/140‑1 (MQ=255) gagaCCTTCACCGTCGGTGCATTGTCCAGCGGCAAAGAAACCATGAAACCCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGAt < 2:565742/143‑1 (MQ=255) aggccTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATc < 1:173583/140‑1 (MQ=255) tCGGTTCATTGGCCGGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATc < 1:506418/143‑1 (MQ=255) ttCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGt < 1:255641/143‑1 (MQ=255) tttGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTgg > 1:875323/1‑143 (MQ=255) cAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTcgcg > 1:597930/1‑143 (MQ=255) aGCGGGAAAGAAACCCGGAATCGCGCTGAGCTGCTGGAAGCCCTCAAGCAATCCTACTGCGGCCCGATTGGTGCCGATTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAGCAGCGTATCGAGTCTGGTcgcgc < 2:302391/143‑1 (MQ=255) cGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGa > 1:428874/1‑143 (MQ=255) gCAAAGAGACCATGGAATTTTTCAAGCTGCTGGAAGCCCTCAAGCCAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACt < 1:846946/143‑1 (MQ=255) gCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGGTCCAACAGCGTATCGAGTCCGGTCGCGCGACt > 1:460463/1‑143 (MQ=255) cAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCCCAAGCAAACCTACTGCGGCGCGGGTGGTGCCGAGAATATGCACATTGCGAGCACCGAAGAAAAACGCTGGATCCCACAGCGCACCGAGTCTGGGCCCGCGACta > 2:191741/1‑142 (MQ=255) aacgAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACttt < 1:50481/140‑1 (MQ=255) gAAACCATGAAACCCGGCGAGCCGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCaa < 1:65110/143‑1 (MQ=255) aaaCCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAAt > 1:254197/1‑143 (MQ=255) cATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTCACAATAGCg > 2:220166/1‑143 (MQ=255) aCTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGAACCGAAGAAAAACTCTGGATcc < 1:87696/98‑1 (MQ=255) aCTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGAACCGAAGAAAAACTCTGGATcc > 2:87696/1‑98 (MQ=255) tCGGCGAGCTGCTGGAAGCCCTCAAGCAAAACTACTGGGGAACGATTGTTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGCATGGTCGCCCGACTTGCAATAACGAAGAGaaa > 2:644529/1‑143 (MQ=255) gCGAGCGGCTGGACGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCATCACCGACGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGaaaaaa < 1:705990/143‑1 (MQ=255) ggAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCACTAGCGAAGAGAAAAAGCGCTTCTTaa > 1:829613/1‑143 (MQ=255) gAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGGGCGGCTCTAAATATCGTAGAGAAAAAACGCTTCTTAAg > 1:374669/1‑143 (MQ=255) aGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCg > 1:303552/1‑143 (MQ=255) aGCCCGCGAGCCAACCTTCTGTGGCCCGTTTGGTGCCGACGATACGCACATTGCCAGCACCGAACAACAACTCTGGATCCTACCGCGTATCTAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCg < 2:643458/143‑1 (MQ=255) cTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCCCATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGCCTGGTCGCGCGACTTTCAATAGCGCAGCGGAAAAACGCTTCTTACGCGCACt > 1:439381/1‑143 (MQ=255) tCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAACACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTCTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTg > 2:864443/1‑143 (MQ=255) tgaaaaCTACTCCAGCGGGATTGCCGCCGACGATAGTCACATAACCAGAAACAAAAAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAccg < 2:657608/141‑1 (MQ=255) aaCCTACTGCGGCCCGATTGGTGCCGAGTATATGAACATTACCCGCACCGACTAAAAACTCTGGATCCAACGGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAccgccg > 1:322803/1‑143 (MQ=255) aCTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCGGCACCGAGGAAAAACTCTGGATACAACAGCGTATCGACTCTGGTCGCGCGGCTTTCAATAGAGACTAGAACAAACGCTTCTGAAGACAACTGgccgccacagac > 2:64404/1‑137 (MQ=255) aCTGCGGCCCGATTGGTGCCGAGTATACGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGaa < 1:724895/143‑1 (MQ=255) ggTGCCGAGTATACCCACTTCACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTa < 2:428493/143‑1 (MQ=255) ccGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTc > 1:877927/1‑143 (MQ=255) ttcacaCTACCTGCACCGAAGTAGACCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCaaaa < 2:567150/141‑1 (MQ=255) cacaTTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGCCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAACACGCTTCTTACGCGAACTGACCGCCGCTGAAGGTCTTGCACGTTACCCCCGCGCaacgca > 2:40548/1‑139 (MQ=255) agtACCTGCAGCGAAGAAAGTCGCTGGATCCAACGGCGTATCGAGTCTGGTCGCGCGACTTTCCATCGCGATAATAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTccc < 2:271168/141‑1 (MQ=255) aCCAGCACCGAAGAAAAGCTCTGGAGCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAAGTCCCTgg < 2:260632/143‑1 (MQ=255) ccAGCCCCGGACAAAAGCGCTGGATCCAACTGCGTATCGAGTGTTTTCGCGCGACTATCACCAGACATGAGAAAACCCGCTTCTTACGCGAACCGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgc < 2:677471/143‑1 (MQ=255) cAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACg > 1:76707/1‑117 (MQ=255) cAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAACATTCCCTGgcg > 1:815366/1‑143 (MQ=255) cAGCACCGAAGAAAAACTCTGGATCCAACAGCGGATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACg < 2:76707/117‑1 (MQ=255) aggacggaagaaAGACTCTGGATCCAGCAGCGTGTCGAGTTGGGTCGCGGGACTTGCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGGAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgcgc < 2:194955/137‑1 (MQ=255) gaagaaAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAACGCCCTGGCGCCCAACg > 1:216845/1‑143 (MQ=255) aagaaAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGGGGGTGTTTCCATAGCGAAGAGCAAAAACGCTTCTTAAGCGGACTGACCGCAGCTGCAGGTCTTGCACGTTCCCTCGGCTCCAAATCCCCTGGAGCACAAccc > 2:494639/1‑141 (MQ=255) gaaAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCtt > 1:621757/1‑143 (MQ=255) aaaaaCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGACTTGAACGGTACCTCGGCGCAAAAGTCCCGCGCGCAAACAGCTtc > 2:652700/1‑143 (MQ=255) aaaCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTCCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTAACCTCGGCGCAAAATTCCCTTGCGCAAAACGCTtctc > 2:313396/1‑143 (MQ=255) | CCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTC > NC_000913/759110‑759388 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |