Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,196,232	C→T	T370T (ACC→ACT)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,232	0	C	T	100.0%	71.0 / NA	30	T370T (ACC→ACT)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/11);  total (19/11)

TGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGA  >  NC_000913/1196094‑1196315                                                                                                                                            |                                                                                    tGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAg                                                                                 >  1:460804/1‑143 (MQ=255)        aaCGTCGGTGACGAATGCGCGCTGTTTTAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGAGACATATAGTTTGGACTGAAGCGGCTGa                                                                          <  2:550660/143‑1 (MQ=255)             cGGGGACGAATGCCCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGGAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAcctga                                                                     <  2:14507/143‑5 (MQ=38)                    gAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaa                                                              <  1:166104/143‑1 (MQ=38)                     aaTGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa                                                             <  2:666444/143‑1 (MQ=255)                      aTGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAg                                                            <  1:532838/143‑1 (MQ=38)                       tGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAgg                                                           >  2:511700/1‑143 (MQ=255)                          gcCCTGTGTGAAGCCACCCACGGTACTGCGCCGAAATACGCCGG‑CAGGCAAATGGGAAACCCTGGCTCTAGTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTAt                                                        <  1:855776/143‑1 (MQ=18)                              tGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGG‑ACACAGTACACCCCTTTATTTTTATTCTCTCCGCTGAGGTGATGCTGCGCCATATGGGTTGGACTGACGCGGCTGACCTGATTGTTCACGGTATGGaa                                                    >  1:856222/1‑143 (MQ=16)                              tGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGCAGCGGCTGACCTGATTGTTAAAGGGATGGaa                                                    >  1:594511/1‑143 (MQ=37)                               gTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAg                                                   >  1:310542/1‑143 (MQ=37)                                     aGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCaa                                             <  2:698613/143‑1 (MQ=255)                                       ccacccacGGTACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAACTCCTGGCTCTATTATTCTCTCCCCTGAGATGATGCTGCGCCATATGAGTTGGACTGAAGCGGCTGACCTAATTGTTAAAGGTATGGAAGGCGCAATc                                           >  1:354571/1‑143 (MQ=25)                                       ccacccacGGTACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATc                                           >  1:479938/1‑143 (MQ=25)                                         acccacGGTACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaa                                         <  1:217141/143‑1 (MQ=25)                                          cccacGGTACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaat                                        >  2:11716/1‑142 (MQ=255)                                                 tACTGCGCCGAGAGATGCCGGGCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgccaaga                                 <  1:227009/143‑6 (MQ=18)                                                 tACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTTAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAGTCAATGCCaaga                                 >  2:687155/1‑141 (MQ=21)                                                 tACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGACTGTTAAAGGTATGGAAGGCGCAATCAAtgccaaga                                 >  2:71997/1‑138 (MQ=21)                                                    tGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCg                              >  1:82858/1‑143 (MQ=18)                                                         cGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAAct                         >  1:233744/1‑142 (MQ=18)                                                            actctGCCGGTCAGG‑GATAAGTAAATCCTGGCTCTATTGTGCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTAt                      <  2:825624/139‑1 (MQ=11)                                                                  ccGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCTGCTGACCTGATTGTTAAAGGTATGGAGGGCGCAAGCACTGCCAAGACCGTAACTCATGACTTc                >  2:902970/1‑143 (MQ=17)                                                                   cGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATCTGGGTTGGACTGAAGCGGCTGACCTGATTGTTAGAGGCATGGACGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCg               >  2:483675/1‑143 (MQ=17)                                                                    ggTCAGG‑ATAAAGTGAATCCTTGCGCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAGGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGa              <  2:871780/143‑1 (MQ=11)                                                                      tCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGaac            >  1:570497/1‑141 (MQ=14)                                                                          g‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTCAAGGTATGGAAGGCGCACTCAATGCCAAGACCGTAACTTATGCCTTCGAACGTCt        >  1:357583/1‑143 (MQ=11)                                                                          g‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCt        >  1:229535/1‑143 (MQ=14)                                                                               aaGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTgatg    >  1:60853/1‑143 (MQ=11)                                                                                 gTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGCTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAGCGTCTgatgga  >  2:134127/1‑143 (MQ=14)                                                                                                                                            |                                                                                    TGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGA  >  NC_000913/1196094‑1196315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑