Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,196,247	A→G	L375M (TTA→CTG)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,247	0	A	G	96.3%	56.9 / NA	27	L375M (TTA→CTG)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (17/9);  total (18/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGA  >  NC_000913/1196113‑1196315                                                                                                                                        |                                                                     gAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaa                                                              <  1:166104/143‑1 (MQ=38)  aaTGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa                                                             <  2:666444/143‑1 (MQ=255)   aTGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAg                                                            <  1:532838/143‑1 (MQ=38)    tGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAgg                                                           >  2:511700/1‑143 (MQ=255)       gcCCTGTGTGAAGCCACCCACGGTACTGCGCCGAAATACGCCGG‑CAGGCAAATGGGAAACCCTGGCTCTAGTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTAt                                                        <  1:855776/143‑1 (MQ=18)           tGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGG‑ACACAGTACACCCCTTTATTTTTATTCTCTCCGCTGAGGTGATGCTGCGCCATATGGGTTGGACTGACGCGGCTGACCTGATTGTTCACGGTATGGaa                                                    >  1:856222/1‑143 (MQ=16)           tGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGCAGCGGCTGACCTGATTGTTAAAGGGATGGaa                                                    >  1:594511/1‑143 (MQ=37)            gTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAg                                                   >  1:310542/1‑143 (MQ=37)                  aGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCaa                                             <  2:698613/143‑1 (MQ=255)                    ccacccacGGTACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAACTCCTGGCTCTATTATTCTCTCCCCTGAGATGATGCTGCGCCATATGAGTTGGACTGAAGCGGCTGACCTAATTGTTAAAGGTATGGAAGGCGCAATc                                           >  1:354571/1‑143 (MQ=25)                    ccacccacGGTACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATc                                           >  1:479938/1‑143 (MQ=25)                      acccacGGTACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaa                                         <  1:217141/143‑1 (MQ=25)                       cccacGGTACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaat                                        >  2:11716/1‑142 (MQ=255)                              tACTGCGCCGAGAGATGCCGGGCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgccaaga                                 <  1:227009/143‑6 (MQ=18)                              tACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTTAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAGTCAATGCCaaga                                 >  2:687155/1‑141 (MQ=21)                              tACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGACTGTTAAAGGTATGGAAGGCGCAATCAAtgccaaga                                 >  2:71997/1‑138 (MQ=21)                                 tGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCg                              >  1:82858/1‑143 (MQ=18)                                      cGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAAct                         >  1:233744/1‑142 (MQ=18)                                         actctGCCGGTCAGG‑GATAAGTAAATCCTGGCTCTATTGTGCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTAt                      <  2:825624/139‑1 (MQ=11)                                               ccGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCTGCTGACCTGATTGTTAAAGGTATGGAGGGCGCAAGCACTGCCAAGACCGTAACTCATGACTTc                >  2:902970/1‑143 (MQ=17)                                                cGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATCTGGGTTGGACTGAAGCGGCTGACCTGATTGTTAGAGGCATGGACGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCg               >  2:483675/1‑143 (MQ=17)                                                 ggTCAGG‑ATAAAGTGAATCCTTGCGCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAGGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGa              <  2:871780/143‑1 (MQ=11)                                                   tCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGaac            >  1:570497/1‑141 (MQ=14)                                                       g‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTCAAGGTATGGAAGGCGCACTCAATGCCAAGACCGTAACTTATGCCTTCGAACGTCt        >  1:357583/1‑143 (MQ=11)                                                       g‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCt        >  1:229535/1‑143 (MQ=14)                                                            aaGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTgatg    >  1:60853/1‑143 (MQ=11)                                                              gTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGCTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAGCGTCTgatgga  >  2:134127/1‑143 (MQ=14)                                                                                                                                        |                                                                     GAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGG‑ACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGA  >  NC_000913/1196113‑1196315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑