| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,019,665 | Δ1 bp | 25.6% | coding (42/606 nt) | ubiJ → | ubiquinone biosynthesis accessory factor UbiJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,019,665 | 0 | G | . | 25.6% | 80.5 / 36.8 | 43 | coding (42/606 nt) | ubiJ | ubiquinone biosynthesis accessory factor UbiJ |
| Reads supporting (aligned to +/- strand): ref base G (13/16); new base . (6/5); total (21/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.79e-01 | |||||||||||
CATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGCAGGGGTTGTGGCGCTGCATCGTGGTTATAAGTTCTGACAGGAGACCGGAAATGCCTTTTAAACCTTTAGTGACGGCAGGAATTGAAAGTCTGCTCAACACCTTCCTGTATCGCTCACCCGCGCTGAAAACGGCCCGCTCGCGTCTGCTGGGTAAAGTATTGCGCGTGGAGGTAAAAGGCTTTTCGACGTCATTGATTCTGGTGTTCAGCGAACGCCAGGTTGATGTACTGGGCG > NC_000913/4019523‑4019807 | cATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGCAGGGGTTGTGGCGCTGCATCGTGGTTATAAGTTCTGACAGGAGACCGGAAATGCCTTTTAAACCTTTAGTGACGGCAGGAATTGAAAGTCTg < 1:622942/143‑1 (MQ=255) atgatgCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGCAGGGGTTGTGGCGCTGCATCGTGGTTATAAGTTCTGACAGGAGACCGGAAATTCCTTTTAAACCTTTAGTGACGGCAGGAATTGAAGGTGTGc > 2:70047/1‑143 (MQ=255) gCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGCAGGGGTTGTGGCGCTGCATCGTGGTTATAAGTTCTGACAGGAGACCGGAAATGCCTTTTAAACCTTTAGTGACGGCAGGAATTGAAAGTCTGCTCAac < 1:543317/143‑1 (MQ=255) aTGCCGGATTCGAAAGTGACGACTACTACAATCTGACGGCAGGGGTTGTGGCGCTGCATCGAGGTTATAAGTTCTGACAGGAGACCGGAAATGCCTTTTAAACCTTTACTGACGGCAGGACTTGAAAGTCTGCACAacccatt > 1:869749/1‑138 (MQ=255) tGCGGGATTCGAGAGTGTCGACTACTACAGTCGGACGGCAGGGGTTGTGGCGCTGCATCGTGGTTATAAGTTCTGACAGGAGACCGGAAATGCCTTTTAAACCTTTAGTGACGGCAGTAATTGAAAGTCTGCTCAACACCTTc < 2:611872/143‑1 (MQ=255) cGGATTCGAAAGTGTCGACTACTACAATCTGACGGCAGGGGTTGTGGCGCTGCATCGTGGTTATAAGTTCTGGCAGGAGACCGGAAATGCCTTTTAAACCTTTAGTGACGGCAGGAATTGAAAGTCT‑CTCAACACCTTCCTGt > 1:733204/1‑143 (MQ=255) atccGAAAGTGTCGGCCACTACAATCTCACGCCAGGCGTTGTGGCGCTGCATCGTGGTTATAAGTTCTGACAGGAGACCGGAAATGCCTTTTAAACCTTTAGTGACGGCAGGAATTGAAAGTCT‑CTCAACACCTTCCTGTATc < 1:476233/140‑1 (MQ=255) cGACTACTACAATCTGACGGCAGGGGTTGTGGCGCTGCATCGTGGGTATAAGTTCTGACAGGAGACCGGAAATGCCTTTTAAACCTTTAGTGACGGCAGGAATTGAAAGTCTGCTCAGCACCTTCCTGTATCGCTCACCcgcg > 1:102521/1‑143 (MQ=255) gACTACTACAATCTGACGGCAGGGGTTGTGGCGCTGCATCGTGGTTATAAGTTCTGACAGGAGACCGGAAATGCCTTTTAAACCTTTAGTGACGGCAGCAATTGAAAGTCTGCTCAACACCTTCCTGTATCGCTCACCCgcgc > 2:387309/1‑143 (MQ=255) actactACAATCTGACGGCAGGGGTTGTGGCGCTGCATCGTGGTTATAAGTTCTGACAGGAGACCGGAAATGCCTTTTAAACCTTTAGTGACGGCAGGAATTGAAAGTCTGCTCAACAACTTCCTGTGTCGCCCACCCGCGCt > 1:50852/1‑143 (MQ=255) cttgatgcTGACGGCATGGGTTGTGGCGCTTCATCGCGGTTATACGTTCTGACAGGAGACCGGAAATGCCTTTTAACCCTTTAGTGACGGCAGGAATTGAAAGTCT‑CTCAACACCTTCCTGTATCGCTCACCCGCGCTGaaaa < 2:778/136‑1 (MQ=255) tacAATCTGACGGCAGGGGTTGTGGCGCTGCATCGTGGTTATAAGTTCTGACAGGAGACCGGAAATGCCTTTTAAACCTTTAGTGACGGCAGGAATTGAAAGTCT‑CTCAACACCTTCCTGTATCGCTCACCCGCGCTGAacac > 1:924029/1‑143 (MQ=255) gaggcTGACGGCAGGGGTTGTGGCGCTGCATCGTGGTTATAAGTTCTGACCGGAGACCGGAGATGCCTTTTAAACCTTTCCTGACGGCAGGAATTGAAAGTCTGCTCAACACCTTCCTGTATCGCTCACCCGCGCTGAAAACg < 2:455902/139‑1 (MQ=255) cgtcAGGCGGTGTGGCGCTGCATTGGGGTTATCAGTTCCTGCAGGAGCCCGGAACTGCCTTTTGTCCCTTTAGTGACGGCAGGAATTGATAGTCTGCTCACCTCCTTCCTGTATCGCTCACCCGCGCTGAAAACGGCCCGCTc < 2:819342/140‑1 (MQ=255) gTTGTGGCGCTGCATCGTGGTTATAAGTTCTGACAGGAGACCGGAAATGCCTTTTAAACCTTTAGTGACGGCAGGAATTGAAAGTCTGCTCAACACCTTCCTGTATCGCTCACCCGCGCTGAAAACGGCCCGCTCGCGTctgc < 1:712871/143‑1 (MQ=255) ttGTGGCGCTGCATCCTGCGTATAAGTTCTGACAGGAGACCGGAAATGCCTTTTAAACCTTTAGTGACGGCAGGAATTGAAAGTCTCCTCACCCCCGCCAAAAACCGTTCGCCCTCGCTGAAAACGGCCCGCTCGCGTctgct < 1:178936/143‑1 (MQ=255) tgGCGCTGCATCGTGGTTATAAGTTCTGACAGGAGACCGGAAATGCCTTTTAAACCTTTAGTGACGGCAGGAATTGCACGTCTCCTCAACATCTTTCTGTATCGCTCACCCGCGCTGAAAACGGCCCGCTCGCGTCTGCTagg > 1:348523/1‑143 (MQ=255) gtggCAGCGTGGTGATGAGTTGTGACGGGAGAGGGGAAGTGCCTGTTAAACGTTGAGTGAGGGCAGGGATGGAAAGTCTGCGCAACACGTTCCTGTGTCGCGGACGCGCGCTGAAGACGGCCCGCTCGCGTCTGCTGGGTaaa < 2:790237/140‑1 (MQ=255) tGGTTAGAAGTTCGGACAGGAGACCGGAAATGCCTTTTAAACCTTTAGTGACGGCAGGAATTGAAAGGCTGCGCAACGCCTTCCTGTATCGCTCACCCGCGCTGAAAACGGCCCGCTCGCGTCTGCTGGGTAAAGTATTgcgc < 1:749805/143‑1 (MQ=255) ttATAAGTTCTGACAGGAGACCGGAAATGCCTTTTAAACCTTTAGTGACGGCAGGAATTGAAAGTCTGCTCAACACCTTCCTGTATCGCTCACCCGCGCTGAAAACGGCCCGCTCGCGTCTGCTGGGTAAAGTATTGcgcggt > 1:795982/1‑141 (MQ=255) ttATAAGTCCTGACAGGAGACCGGAGATGCGTTGTAAGCCTTTAGTGACGGCAGGAAGTGAAAGTCTGCTCAACACCTTCCGGTATCGCTCACCCGCGCTGAAAACGGCCCGCTCGCGTCTGCTGGGTAAAGTATTGCGCGTg < 2:191566/143‑1 (MQ=255) aaGTTCTGACAGGAGACCGGAAATGCCTTTTAAACCTTTAGTGACGGCAGGAATTGAAAGTCT‑CTCAACCCCTTCCTGTATCGCTCACCCGCGCTGAAAACGGCCCGCTCGCGTCTGCTGGGTAAAGTATTGCGCGTGGAGGt < 2:578484/143‑1 (MQ=255) gTTCTGACAGGAGACCGGAAATGCCTTTTTAACCTTTAGTGACGGCAGGAATTGAAAGTCTGCTCAACACCTTCCTGTATCGCTCACCCGCGCTGAAAACGGCCCGCTCGCGCCTGGTGGGTGGAGTATTGCGCGGGGAggca > 1:801714/1‑141 (MQ=255) ttCTGACAGGAGACCGGAAATGCCTTTTAAACCTTTAGTGACGGCAGGAATTGAAAGTCT‑CTCAACACCTTCCCGCCCAACTCGCCCCCGCCGAAAACGGCCCGCCCGCGTCTGCTGCGTAAAGTATTGCGCGCGGAGGTaac > 1:937875/1‑142 (MQ=255) cTGACAGGAGACCGGAAATGCCTTTTAAACCTTTAGTGACGGCAGGAACTGAAAGTCTGCTCAACACCTTCCTGTATCGCTCACCCGCGCTGAAAACGGCCCGCTCGCGTCTGCTGGGTAAAGTATTGCGCGTGGAGGTaaaa < 2:664168/143‑1 (MQ=255) gACAGGAGACCGGAAATGCCTTTTAAACCTTTAGTGACGGCAGGAAGTGAAAGTCTACCCAACACCTTTCGATATCGCTCACCCGCGCTGAAAACGGCCCGCTCGCGTCTGCTGGTTCAAGTATTGCGCGTGGAGGGAAAAgg > 1:132289/1‑143 (MQ=255) ggAGACCGGAAATGCCTTTTAAACCTTTAGTGACGGCAGGAATTGAAAGTCT‑CTCAACACCTTCCTGTATCGCTCACCCGCGCTGAAAACGGCCCGCTCGCGTCTGCTGGGTCAAGTATTGCGCGTGGAGGTAAAAGGCtttt > 1:152213/1‑143 (MQ=255) gagaCCGGAAATGCCTTTTAAACCTTTAGTGACGGCAGGAATTGAAAGTCT‑CTCAACACCTTCCTGTATCGCTCACCCGCGCTGAAAACGGCCCGCTCGCGTCTGCTGGGTAAAGTATTGCGCGTGGAGGTAAAAGGCTTTTc < 2:924029/143‑1 (MQ=255) gagaCCGGAAATGCCTTTTAAACCTTTAGTGACGGCAGGAATTGAAAGTCT‑CTCAACACCTTCCTGTATCGCTCACCCGCGCTGAAAACGGCCCGCTCGCGTCTGCTGGGTAAAGTATTGCGCGTGGAGGTAAAAGGCTTTTc < 1:289492/143‑1 (MQ=255) ccGGAAATGCCTTTTAAACCTTTAGTGACGGCAGGAATTGAAAGTCTGCTCAACACCTTCCTGTATCGCTCACCCGCGCTGAAAACGGCCCGCTCGCGTCTGCTGGGTAAAGTATTGCGCGTGGAGGTAAAAGGCTTTTCGAc < 2:658905/143‑1 (MQ=255) cGGAAATGCCTTTTAAACCTTTAGTGACGGCAGGAATTGAAAGTCT‑CTCAACACCTTCCTGTATCGCTCACCCGCGCTGAAAACGGCCCGCTCGCGTCTGCTGGGTAAAGTATTGCGCGTGGAGGTAAAAGGCTTTTCGGCgc > 2:259404/1‑142 (MQ=255) aaTGCCTTTTAAACCTTTAGTGACGGCAGGAATTGAAAGTCTGCTCAACACCTTCCTGTATCGCTCACCCGCGCTGAAAACGGCCCGCTCGCGTCTGCTGGGTAAAGTATTGCGCGTGGAGGTAAAAGGCTTTTCGACGTCAt < 1:13545/143‑1 (MQ=255) tGCCTTTTAAACCTTTAGTGACGGCAGGAATTGAAAGTCTGCTCAACACCTTCCTGTATCGCTCACCCGCGCTGAAAACGGCCCGCTCGCGTCTGCTGGGTAAAGTATTGCGCGTGGAGGTAAAAGGCTTTTCGACGTCAtgg > 1:807516/1‑141 (MQ=255) ccTTTTAAACCTTTAGTGACGGCAGGAATTGAAAGTCTGCTCAACACCTTCCTGTATCGCTCACCCGCGCTGAAAACGGCCCGCTCGCGTCTGCTGGGTACAGTAGTGCGCGTGGAGGACAAACACTTCTCGCCGTCaccgat > 2:863318/1‑138 (MQ=255) aaCCTTTAGTGACGGCAGGAATTGAAAGTCTGCTCAACACCTTCCTGTATCGCTCACCCGCGCTGAAAACGGCCCGCTCGCGTCTGCTGGGTAAAGTATTGCGCGTGGAGGTAAAAGGCTTTTCGACGTCATTGATTCTGgtg > 1:505364/1‑143 (MQ=255) gtAGTGACGGCAGGAATGGAAAGCCTGCTCAACACCTTCCTGTATCGCTCACCCGCGCTGAAAACGGCCCGCTCGCGTCTGCTGGGTAAAGTATTGCGCGTGGAGGTAAAAGGCTTTTCGACGTCATTGATTCTGGTGTTCAg < 1:561671/142‑1 (MQ=255) gACGGCAGGAATTGAAAGTCTGCTCAACACCTTCCTGTATCGCTCACCCGCGCTGAAAACGGCCCGCTCGCGTCTGCTTGGTAAAGTATTGCGCGTGGAGGTACAAGGCTTTTCGACGTCATTGAGTCTGGTGTTCACCGAAc > 1:191136/1‑143 (MQ=255) cAGGAATTGAAAGTCTGCTCAACACCTTCCTGTATCGCTCACCCGCGCTGAAAACGGCCCGCTCGCGTCTGCTGGGTAAAGTATTGCGCGTGGAGGTAAAAGGCTTTTCGACGTCATTGATTCTGGTGTTCAGCGAACGCCAg < 1:24670/143‑1 (MQ=255) aGGAATTGAAAGTCT‑CTCAACACCTTCCTGTATCGCTCACCCGCGCTGAAAACGGCCCGCTCGCGTCTGCTGGGTAAAGTATTGCGCGCGGAGGTAAAAGGCTTTTCGACGTCATTGATTCTGGTGGTCAGCGACCGCCAggg > 2:651741/1‑142 (MQ=255) aGGAATTGAAAGGCCGGTCGGCGCCTTCCTGTATCGCTCACCCGGGCTGAAAGCGGCCCGCTCGCGTCTGCTGGGTAAAGTATTGCGCGTGGAGGTAAAAGGCTTTTCGACGTCATTGATTCTGGTGTTCAGCGAACGCCAgg < 2:550689/143‑1 (MQ=255) gAATTGAACGTCTGCTCCGCCCCTTCTTGTATCGCTCACCCGCGCTGCAAACGGCCCGCTCGCGTCTGCTTGGTAAAGTATTGCGCGTGGAGGTAAAAGGCTTTTCGACGTCATTGATTCTGGTGTTCAGCGAACGCCAGGtt < 2:698287/143‑1 (MQ=255) aaaGTCTGCTCAACACCTTCCTGTATCGCTCACCCGCGCTGAAAACGGCCCGCTCGCGTCTGCCGGGTACAGTACATCGCGTGGAGGTAAAAGGCTTTTCGACGTCATTGATTCTGGTGTGCAGCGAACGCCAGGTGGATCTa > 2:567872/1‑143 (MQ=255) tCTGCTCAACACCTTCCTGTATCGCTCACCCGCGCTGAAAACGGCCCGCTCGCGTCTGCTGGGTAAGGTATGGCGCGTGGAGGTAAAAGGCTTTTCGACGTCATTGATTCTGGTGTTCAGCGAGCGCCAGGTTGATGTACTgt > 2:870180/1‑142 (MQ=255) tCTGCTCAACACCTTCCTGTATCGCTCACCCCCGCTGAAACCGGCCCGCTCGCGTCTGCTGGGTAAAGTATTGCGCGTGGAGGTAAAAGGCTTTTCGACGTCATTGATTCTGGTGTTCAGCGAACGCCAGGTTGATGTACTgg < 1:550814/143‑1 (MQ=255) gCTCAACACCTTCCTGTATCGCTCACCCGCGCTGAAAACGGCCCGCTCGCGTCTGCTGGGTAAAGTATTGCGCGTGGAGGTAAAAGGCGTTTCGACGTCATTGATGCTGGTGTTCAGCGAACGCCCGGTGGATGGACTAGGCg > 2:650773/1‑143 (MQ=255) | CATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGCAGGGGTTGTGGCGCTGCATCGTGGTTATAAGTTCTGACAGGAGACCGGAAATGCCTTTTAAACCTTTAGTGACGGCAGGAATTGAAAGTCTGCTCAACACCTTCCTGTATCGCTCACCCGCGCTGAAAACGGCCCGCTCGCGTCTGCTGGGTAAAGTATTGCGCGTGGAGGTAAAAGGCTTTTCGACGTCATTGATTCTGGTGTTCAGCGAACGCCAGGTTGATGTACTGGGCG > NC_000913/4019523‑4019807 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |