Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,196,268 Δ1 bp 10.8% intergenic (‑169/+64) thiC ← / ← rsd phosphomethylpyrimidine synthase/regulator of sigma D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,196,2680C.10.8% 154.8 / 18.0 65intergenic (‑169/+64)thiC/rsdphosphomethylpyrimidine synthase/regulator of sigma D
Reads supporting (aligned to +/- strand):  ref base C (36/21);  new base . (4/3);  total (41/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.91e-01

TTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGC  >  NC_000913/4196129‑4196410
                                                                                                                                           |                                                                                                                                              
ttGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTACGGCACTCCGa                                                                                                                                             >  2:895317/1‑143 (MQ=255)
   tCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTATTGTTCCCTTCGCAGGTAGTAGCCTGATAAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGAcga                                                                                                                                          >  1:676758/1‑141 (MQ=255)
   gcAGCCGACGGAGGAAGCAAGAGACGATCGCCGCAGGCGCGATGCGATAGCAGATTAACTCTTGTTCCCTGCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACaa                                                                                                                                          <  2:846488/142‑1 (MQ=255)
    cAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCAGATCCCGAATAAACGGTCGCAGCCAGTTAAGGCACT‑‑GACAAGaa                                                                                                                                       >  2:450260/1‑143 (MQ=255)
      gacgacGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACCAGaa                                                                                                                                       >  1:457530/1‑143 (MQ=255)
         gacgGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAAtt                                                                                                                                    <  2:519518/143‑1 (MQ=255)
           cgGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAg                                                                                                                                  <  1:216290/143‑1 (MQ=255)
            ggATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAga                                                                                                                                 >  1:693624/1‑142 (MQ=255)
              aTGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGccc                                                                                                                               <  1:265984/143‑1 (MQ=255)
                gAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACCAGAAATTAGCCCCg                                                                                                                             >  2:489728/1‑143 (MQ=255)
                  aGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTACGGCACTCCGACCAGAAATTAGCCCCGcc                                                                                                                           >  2:865961/1‑142 (MQ=255)
                   gCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCaa                                                                                                                          >  1:97952/1‑143 (MQ=255)
                    cAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACCCCGACAAGAATTCAGCCCCGCaaa                                                                                                                         >  2:869472/1‑143 (MQ=255)
                        aggcGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATggg                                                                                                                     <  1:172763/140‑1 (MQ=255)
                        agaCGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCGGGTTCCGCGGATCCCGAAAAAACGGACTTAGCCGGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATggg                                                                                                                     >  2:247712/1‑143 (MQ=255)
                         ggcGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATgggg                                                                                                                    <  2:110843/141‑1 (MQ=255)
                               cgccgcAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGa                                                                                                              <  1:765334/143‑1 (MQ=255)
                                gccgccGGGGCGATGCGCTAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGaa                                                                                                             <  2:502812/143‑1 (MQ=255)
                                 ccgcAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAAt                                                                                                            <  1:505128/143‑1 (MQ=255)
                                  cgcAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATGGAATg                                                                                                           >  1:494260/1‑143 (MQ=255)
                                    cgggggCGGTTCGATGGCAGATTAACTCTTGTTCCCTTTGCAGGGATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGGCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTa                                                                                                         <  2:651492/141‑1 (MQ=255)
                                      ggggggAGGCGATAGCAGATTGACTCGTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAAGAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTaaa                                                                                                       <  2:423772/143‑1 (MQ=255)
                                         gcgatgcgatAGCAGATTAACTCTTGTTCCCTTGGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTGAGCCAGTTAAGGTACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTAcg                                                                                                  <  2:242320/143‑1 (MQ=255)
                                            atgcgatAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCACCGCAAATGGGGCAGTGAATGTAACTTAagc                                                                                                 >  2:403704/1‑143 (MQ=255)
                                                gatAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAAATAAGGCACTCCGACAAGAAATTAGCCCCGCACATGGGGCACTGCATGTAACTTACACGTTa                                                                                             >  1:83217/1‑143 (MQ=255)
                                                     cAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCAGTGAATGTAAATTACGCGTTAACAgagc                                                                                      >  2:396679/1‑143 (MQ=255)
                                                      aGATTAGCTCTCGTACCCTTCGCAGGTATTAGCCACAAAAGAATACGCTGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCa                                                                                     <  2:915746/143‑1 (MQ=255)
                                                        aTTAACGCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCa                                                                                     <  1:829593/143‑1 (MQ=255)
                                                              tctTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTc                                                                               >  1:487548/1‑143 (MQ=255)
                                                              tctTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCACAGGTAATTGTTCATTGAATGTAAATTACGCGTTAACCGCGCAGAACTc                                                                               >  2:663851/1‑143 (MQ=255)
                                                               ctTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAg                                                                            <  1:93286/143‑1 (MQ=255)
                                                                ttGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGCTCCCGAATAAACGGTCTCAGCCCGTTAAGGCACTCCGACAAGAAATTAGCCCCGCCAACGGGGCATTGAATGTAAATCACGCGGCAACAGACCAGCACCCaa                                                                             >  2:452544/1‑143 (MQ=255)
                                                                ttGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGACCTCaa                                                                             >  1:799331/1‑143 (MQ=255)
                                                                ctgtgCCCGTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCaa                                                                             <  1:912107/142‑1 (MQ=255)
                                                                       cTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGACTAAACGGTCTCAGCCAGTTAAGGGACTTCGACAGGAGATTAGCCCCGCAAACGGGGCATTGACTGTAACTTACGCGTTGACAGCGGAGAACTCACGCatgct                                                                      >  1:296372/1‑139 (MQ=255)
                                                                        ttCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCGATGAATGTAAATTACGCGTTAACAGCGCAAAACTCAAGCAGAATg                                                                     >  2:29908/1‑143 (MQ=255)
                                                                        ttCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGCTg                                                                     >  1:507568/1‑143 (MQ=255)
                                                                        ttCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATg                                                                     >  2:22338/1‑143 (MQ=255)
                                                                         tCGCAGGTATTAGCCTGATCAGGTTCCGCGTATCCCGCATAAACGGTCTCAGCCAGTTAAGGCAATCCGACAAGAAATTAGCCCCGCAAATGGGGCATGGAATGTAAAATACGCGTTAACAGCGCAGAACTCAAGCAGGATGt                                                                    >  2:218002/1‑143 (MQ=255)
                                                                             aGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTCAATTCCGCGTTAACAGCGCACAACTCAAGCAAGATGTCTGa                                                                >  1:113300/1‑143 (MQ=255)
                                                                                    aGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGAAAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGCTAACAGCGCCGAACTCAAGAAGGATGTTTGAAgcgagc                                                         >  2:805535/1‑143 (MQ=255)
                                                                                     gCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACgcgggcg                                                        >  2:7744/1‑143 (MQ=255)
                                                                                      ccTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACgcgggcgg                                                       >  1:792042/1‑143 (MQ=255)
                                                                                       cTGATCGGGTTCCGCGGAGCCCGAATAAACGGTCGCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGggcggc                                                      <  1:163217/143‑1 (MQ=255)
                                                                                       cTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGggcggc                                                      >  2:383489/1‑143 (MQ=255)
                                                                                       cTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTGAAGGCACTCCGGGGGGGAACCAACCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGggcggc                                                      <  1:938179/143‑1 (MQ=255)
                                                                                           tCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCa                                                  >  1:101194/1‑143 (MQ=255)
                                                                                            cAGTTTCCGCGGATCCCGAATAAACGGTTTCAGCCATTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCaa                                                 <  2:410468/143‑1 (MQ=255)
                                                                                             aGGTTCCGCGGATCCCGAATAACCGGTCTCAGCCCGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAg                                                <  1:703281/143‑1 (MQ=255)
                                                                                             aGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCCGCCCGCGTCaaa                                                <  1:23631/143‑2 (MQ=255)
                                                                                                ttCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCGAGCACca                                           >  1:808176/1‑143 (MQ=255)
                                                                                                     cgGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACcagca                                        <  2:97952/143‑1 (MQ=255)
                                                                                                            cGAATAAACGGGCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAg                                 <  1:323530/143‑1 (MQ=255)
                                                                                                             gAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTACATTACGCGTAAACAGCGCAGCCCTCGAGCTGGAGGTGTGACGCGGGCGGCGTCAAGCAACAGCAGACTCAGc                                >  1:175080/1‑143 (MQ=255)
                                                                                                                       tctcAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCa                      <  1:383489/143‑1 (MQ=255)
                                                                                                                        ctcAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAg                     >  2:173390/1‑143 (MQ=255)
                                                                                                                           aGCCAGTTAAGGCACTCCGACAAGAAATTAGCGCCGGAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCCGGATGTGTGACGCGAGCGGCGTCAAGCACCAGCAGAATCAGCTGATCCTCCGGcac                  >  2:459837/1‑143 (MQ=255)
                                                                                                                           aGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGcac                  >  2:133699/1‑143 (MQ=255)
                                                                                                                            gCCAGTTAAGGCACT‑‑GACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACcaa               >  2:195973/1‑143 (MQ=255)
                                                                                                                                 ttAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGAGCAGTACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAgcg            >  1:844964/1‑143 (MQ=255)
                                                                                                                                      gCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCCGCTTATCTTCCAGCACAAAGCGCGCtt       >  2:878680/1‑143 (MQ=255)
                                                                                                                                      gCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCtt       >  1:865862/1‑143 (MQ=255)
                                                                                                                                      gCACTCCGACAAGAAATTAGCCCCGCAAATGCGGCATCGACTGCAAATCACGCGCTAACAGCCCAGAACTCAACCAGGACATTCGACGCCGCCGGCGTCAACCACCAGCACAATCACCCAATCCTCCAGCACAACGCCCGCtt       >  2:901603/1‑143 (MQ=255)
                                                                                                                                          tCCGACAAGAAATTAGCCCCGCACATGGGGCATTGAACGTAAATTACGCGTTAACCGCGCAGAACTCCAGCAGGATGGATGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAACGCGCTTcccg   >  2:765153/1‑141 (MQ=255)
                                                                                                                                          tCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGCATCAGCTTATCTTCCAGCACAAAGCGCGCGTCCAg   >  2:294179/1‑143 (MQ=255)
                                                                                                                                           ccGACAAGAACTGAGCCCCGCAAAGGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAgc  <  1:15862/143‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                              
TTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGC  >  NC_000913/4196129‑4196410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑