| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,850,719:1 | +T | 12.5% | intergenic (+26/‑141) | sppA → / → ansA | protease IV, a signal peptide peptidase/L‑asparaginase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,850,719 | 1 | . | T | 12.5% | 126.6 / 15.8 | 40 | intergenic (+26/‑141) | sppA/ansA | protease IV, a signal peptide peptidase/L‑asparaginase 1 |
| Reads supporting (aligned to +/- strand): ref base . (23/12); new base T (3/2); total (26/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.33e-01 | |||||||||||
CCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATC > NC_000913/1850577‑1850859 | ccTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACGTAGTGGCCGACAGAtc > 1:182622/1‑143 (MQ=255) cTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGAtc‑g > 1:634763/1‑143 (MQ=255) gCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTACTGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGGCGTGTACTGAGTGGCCGACCGAtc‑gtc > 2:580207/1‑143 (MQ=255) cACCGCTGGCCTCCGTAGCCTCTACCGCTAAAAGTGGAAGTGACAAGCTGGCCGCGTTTAATGACCCCCAACCCCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGAtc‑gtcg < 1:559718/143‑1 (MQ=255) tcGCTGGGCGCGGTAGCCTGTAAGGTTAAAAGTGAAAGTGTCAAGCCGGCCGCGTTTAATGAGCCACAATACCGGTATGCGTTTTGCCTGACCTGCGCCAACAGGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGc < 2:23210/142‑1 (MQ=255) ccGCTGGCCTCGGTAGCCTCTACCGTTACAAGTGAAAGTGACAGGCTGGCCGCGTTGAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGAC‑GATCTGTCGGc < 2:614290/143‑1 (MQ=255) ccGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGGGACAAGCTAGCCGCGTTGAATGACCAACAAAACCGTTATGCGGTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTTGCCGACAGCta‑gtcgcg > 1:515511/1‑141 (MQ=255) gCTGGCCTCGGGAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTCAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGAC‑GATCTGTCGGCca < 2:369877/143‑1 (MQ=255) tAGCTTCTACGGTTAAAAGCGCAAGGGACACTCCGGCCGCGTCTAATCCCCCACCACACCGTTCTGCGTTGCGCCTGACCCGCGCCAACAGGCGGTAACTCTTGTACTGAGTGGCCGACATAGC‑GTCGGCCACattattcgtt < 2:634074/143‑5 (MQ=255) ctctACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCCTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGt > 1:607582/1‑143 (MQ=255) gTTAAAAGTGAAAGTGACAAGCTGGCAGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGCCTTGTACTGAGTGGCAGACAGATC‑GTCGGCCACATTATTATTTACGTCGACGaa > 2:589948/1‑143 (MQ=255) aaaaGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATcc > 2:917710/1‑143 (MQ=255) aaGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACCTTATTTTTTACGTCGACGAATCCGCGTCCCGCCGTTTc > 2:366068/1‑143 (MQ=255) aaGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTc > 1:780860/1‑143 (MQ=255) gCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGc < 2:481136/143‑1 (MQ=255) tGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTGTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGccc < 1:480908/143‑1 (MQ=255) ccGCGTTTAATGCCCCCCATATCCGTTATGCGCTTTGCCGTCCCTGCGCCCACATGCGTTAAGTCTTGTTCTGAGTCGCCGACAGATC‑TTCGGCCACATTATTTCTTACGTCGACGCATCCTCTTCCCGCTGTTTCGCCCCat < 2:240887/143‑1 (MQ=255) ccGCGCTTAATGCCCCGCAAGCCCGTGATGCGTTTTGCCTGACCTGCGCCCACATGCGTTCGGCCCTGCACTGCCGGGCCCACAGTTC‑GTCGGCTCCATTAGTTTTTAGGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCat < 2:873656/143‑1 (MQ=255) gTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGTCAAGATGCGTTAAGTCTTGTATTGAGTGGCTGACAGATT‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATAttt > 1:623545/1‑143 (MQ=255) gTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATAttt > 2:836492/1‑143 (MQ=255) tGACCCACAAAGCCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTAt < 1:341874/143‑1 (MQ=255) ccACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGTCGACGCCTCCACCCGCTGTTTCGCCCCATATTTCCTTATCCGc > 2:412634/1‑143 (MQ=255) ccACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAAGCCCCTTCCCGCCGATTCGCCACATATTTCCTCCTCCGc > 2:736976/1‑143 (MQ=255) acaAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGcgt > 1:73027/1‑141 (MQ=255) aaaCCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATCATCTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATCTCCTTATCCGCAGAGt > 1:789407/1‑143 (MQ=255) aaCCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATAGTTCCTTGTCTGCATGGTa > 1:491101/1‑143 (MQ=255) gTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAg < 1:917710/143‑1 (MQ=255) cgcCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGt > 1:89455/1‑143 (MQ=255) cgcCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGt > 1:818229/1‑143 (MQ=255) gcCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGta < 1:621024/143‑1 (MQ=255) aCATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGtataga > 1:441371/1‑141 (MQ=255) ttAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGTATATACTTTTGc > 1:871957/1‑143 (MQ=255) ttAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGCGCGCTCCCCCTGCCTCACGTGTATACTTTTGc > 1:880669/1‑143 (MQ=255) ttAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGCTGCGCTCCCCCTGCCTCACGTACCTACTTTTGc > 1:887468/1‑143 (MQ=255) tCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTCCGCTCCCCCTCCCCCACGTACATACTTTTGCTCttt > 2:410634/1‑143 (MQ=255) ttGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCCCACGTACGTCCTTTTGCTCTTTCg > 2:452184/1‑143 (MQ=255) ggcgTGGCCGACAGATC‑GGCGGCCACATTATGTTTTACGTGGACGCATCCTCTTCCCGCTGTTTCGCCCCACATTACCTTGTCCGCGTAGTATCAGGTGCGCTCCCCCTGCCTCCCGTATATACTTTTGCTCTTTCGATAtca < 1:353092/140‑1 (MQ=255) atgTGGCCGAC‑GATCTGTCGGCCACATTATTTTTTACGTCGAAGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCCCTCCCCCCGCCTCACGTATACACTTTTGCTCTTTCGATAtcat > 2:874419/3‑143 (MQ=255) aGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCCTATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATattatt > 1:921540/1‑143 (MQ=255) gTGGCCGAC‑GATCTGTCGGCCACATTATGTTTTACGTCGACGAATCCTCTTGCCCCTGTTTCGCCCCATGTTTCCTTATCCGCATAGTATCAGGTGCGCCCCCCCTACCGCACGCATATACTTTTGCTCTTTCGACAtcctcc > 2:182448/1‑139 (MQ=255) tGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAGGGCTCTTCCCGCTGTTTCGCCCTATATTGCCTTATCCGAATAGTATCAGGTGCGCTCCCACTGCCTCACGTATATCCTTTTGCTCTTTCGCTAtcattca > 1:190817/1‑143 (MQ=255) tGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATGtcattgc > 1:190503/1‑141 (MQ=255) tGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcattca < 2:341225/143‑1 (MQ=255) tGGCCGAC‑GATCTGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGGTTCGCCCCATATTTCCTTATCCGTATAGCATCAGGTGCGCTCCGCCTGCCTCCGCCATCAACCTTTGCTCTCGCGATGtcattct > 2:682382/1‑142 (MQ=255) aTC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCatatcaatatc < 2:634763/143‑1 (MQ=255) | CCTGCACCGCTGGCCTCGGTAGCCTCTACCGTTAAAAGTGAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATC > NC_000913/1850577‑1850859 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |