| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,637,645:1 | +T | 100% | coding (4/1500 nt) | pitA → | metal phosphate:H(+) symporter PitA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,637,645 | 1 | . | T | 95.1% | 111.1 / ‑0.9 | 41 | coding (4/1500 nt) | pitA | metal phosphate:H(+) symporter PitA |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base T (15/24); total (15/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATC‑AAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GC‑T‑ACATTTGTTTGCTGGCCTGGA‑TTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATAC > NC_000913/3637513‑3637775 | agttAATTATTGTTTGCGTGAAAGCCATACCGGGGGCAAATC‑AAACAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTT‑ACAtttgt < 1:337586/140‑1 (MQ=255) tAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATC‑AAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTT‑ACAtttgtttg < 2:827675/143‑1 (MQ=255) attattTTTTGAGTGAAATCCATACAGGGGGCAAATC‑AAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTT‑ACATTTGTTTGCt > 2:191006/1‑143 (MQ=255) tttttCAGTTCACTCCATACGGGGGGCAGATC‑AAAAAAAGTCTATATTTCACTGTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTT‑ACATTTGTTTGCTGGCCt < 2:457889/143‑1 (MQ=255) tgtttGAGTGGAGTCGATACAGGGGGCAAAAC‑AAAAAAAGTCTATATGGCACTTTGCCCGCGCCGCGAGAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTT‑ACATTTGTTTGCTGGCCt < 2:910179/141‑1 (MQ=255) tGAAATCCATACAGGGGGCAAATC‑AAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTT‑CCATTTGTTTGCTGGCTTGGC‑TTTGc > 2:771063/1‑143 (MQ=255) cAGGGGGCAAATC‑AAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTT‑ACATTTGTTTGCTGGCCTGGG‑TTTGCATACCGGGCTg > 1:583654/1‑143 (MQ=255) cAGGGGGCAAATC‑AAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTT‑ACATTTGTTTGCTGGCCCGGA‑TTTGCATACCGGGcgg > 2:207330/1‑141 (MQ=255) cAGGGGGCAAATC‑AAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTT‑ACATGTGTTTGCTGGCCTGGA‑TTTGCATACCGGGCTg > 1:204754/1‑143 (MQ=255) ggaggCAAAAC‑AAAAAAAGTCTATATTTCACCTTTCCCGCGCCGCGAGAGTCACTGATAATGCGCCGCGATCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTT‑ACATTTGTTTGCTGGCCTGGA‑TTTGCATACCGGGCTGtt < 2:104819/140‑1 (MQ=255) ggggCAAATC‑AAAAAAAGTCTATATTTCACTTTGCCCACGCCGCGAAAGTCACTGATAATGCGCCGCGTTCCTGTCCTCACAATGGCGTAACGTCCTAT‑GCTT‑ACATTTGTTTGCTGGCCTGGA‑TTTGCATACCGGGCTGtta > 1:670897/1‑143 (MQ=255) ggCAAATCAAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCAGGTCCTCAAAATGGCGTAACGTCCTAT‑GCTT‑ACATTTGTTTGCTGGCCTGGA‑TTTGCATACCGGGCTGttat < 1:274844/143‑1 (MQ=255) gCAAATC‑AAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTT‑ACATTTGTTTGCTGGCCTGGA‑TTTGCATACCGGGCTGttatta < 1:191006/143‑1 (MQ=255) cAAATC‑AAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAGAATGGCGTAACGTCCTAT‑GCTT‑ACATTTGTTTGCTGGCCTGGA‑CTTGCATACCCGCCTGGtattat > 2:548754/1‑143 (MQ=255) aaaTC‑AAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCACAATGGCGTAACGTCCTAT‑GCTT‑ACATTTGTTTGCTGGCCTGGA‑TTTGCATACCGGGCTGTTattatt < 2:312350/143‑1 (MQ=255) gTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTT‑ACATTTGTTTGCTGGCCTGGA‑TTTGCATACCGGGCTGTTATTATTGCTTGCACTGGc < 2:259307/143‑1 (MQ=255) atatTTCACTTTGCCCGCGCCGCGAAAGTCACTTATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTT‑ACATTTGTTTGCTGGCCTGGA‑TTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCtttt < 2:550490/143‑1 (MQ=255) tatTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTT‑ACGTTTGTTTGCTGGCCTGGA‑TTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTtg > 1:196608/1‑143 (MQ=255) tttCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTT‑ACATTTGTTTGCTGGCCTGGA‑TTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTtgtg < 2:475924/143‑1 (MQ=255) tttCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTT‑ACATTTGTTTGCTGGCCTGAA‑TTTGCATACCGCGCGGTTATTATTGCTTGCACTGGCTTTtctg > 2:79793/1‑143 (MQ=255) ccgcgcgccgggAAAGTCCCTCATAACGCGTCGCGTTTATGACCAGTAGATGATATATCTTCCTGG‑AC‑TCACGTGTGTTTGCGGGCCTGGA‑TTTGCTTACCGGGCTGTTATTTTTGCTTGCACTGGCTTTTGTGCTGTTCTAc < 2:197598/140‑1 (MQ=255) ccgcgccgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTT‑ACATTTGTTTACTGGCCTGGA‑TTTGCATACCGGGCGGTTAGTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGa > 2:760496/1‑143 (MQ=255) cgtgccgcgAAAGTCTCTGCTCCTTCGCCCCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTT‑ACATTTGTTCGCTGGCCTGGA‑TTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGaa < 2:682443/140‑1 (MQ=255) cgcgccgcgACAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTT‑ACATTTGTTGGCTGGCCTGGA‑TTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGaa < 2:263398/143‑1 (MQ=255) ccgcgAAAGTCACTGATACTCCGCCGCGTTCATCTCCTCAAAATGCCGTAACGTCCTAT‑GCTT‑ACATTTGTTTGCTGGCCTGGA‑TTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCa < 1:401711/143‑1 (MQ=255) ccgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTT‑ACATTTGTTTGCTGGCCTGGA‑TTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCa < 1:402161/143‑1 (MQ=255) ccgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTT‑ACATTTGTTTGCTGGCCTGGA‑TTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTATGTGCTGTGCTACgcaacta > 2:532096/1‑137 (MQ=255) cgAAAGTCACTGGTAATGCGCCGGGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTT‑ACATTTGTTTGCTGGCCTGGA‑TTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCa < 2:4357/143‑1 (MQ=255) tGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTT‑ACATTTGTTTGCTGGCCTGGA‑TTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGacac > 1:772640/1‑143 (MQ=255) gtgccgcGTCCATGTCACCAAAATGTTGTAACGTCCTAT‑GCTT‑ACATTTGTTTGCTGGCCTGGA‑TTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGAcaca < 2:267194/141‑1 (MQ=255) ccgcGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GCTT‑ACATTTGTTTGCTGGCCTGGA‑TTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGcc > 1:456065/1‑143 (MQ=255) gcgTTCATGCCCTCAAAATGGCGCAACGTCCTAG‑GCTT‑ACATCTGTTTGCTGGCCTGCA‑TTTGCATACACCGTTGTTATTCTAGCTTGCACGGCCTTTTGTGCTGTTCCAATAAGCCACCAATGGTTTCCATGACACAGCCaa < 2:668402/143‑1 (MQ=255) gtggccccAAAATCGGGTACAGTCCTAT‑GCTT‑ACATTTGTTTGCTGGCCTGGA‑TTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGCGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGt < 1:680760/136‑1 (MQ=255) gccTCAAAATGGCGTAACGTCCTAT‑GCTT‑ACATTTGTTTGCTGGCCTGGATTTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTgg < 1:261627/142‑1 (MQ=255) tCAAAATGGCGTAACGTCCTAT‑GCTT‑ACATTTGTTTGCTGGCCTGGA‑TTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGATGCTCGACGAGGCCATCAATGGTTTCCATGACCCAGCCAACGCCGTGGCAAc > 1:641284/1‑143 (MQ=255) cAAAATGGCGTAACGTCCTAT‑GCTT‑ACATTTGTTTGCTGGCCTGGA‑TTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACGGCCAACGCCGTGGCGAca > 1:345622/1‑142 (MQ=255) tGGCGTAACGGCCTGTGGT‑T‑TAATTTGTTTGCTGGCTTGGG‑TTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTAt < 2:406518/143‑1 (MQ=255) ggCGTAACGTCCTAT‑GCTT‑ACATTTGTTTGCTGGCCTGGA‑TTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATc < 1:624956/143‑1 (MQ=255) gCGTAACGTCCTAT‑GCTT‑ACATTTGTTGGCTGGCTTGTA‑TTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCt < 2:410704/143‑1 (MQ=255) aaCGTCCTAT‑GCTT‑ACATTTGTTTGCTGGCCTGGA‑TTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTCTGTGCTTGTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATAc < 1:846454/143‑1 (MQ=255) aaCGTCCTAG‑GCTT‑ACATTTGTTTGCTGGCCTGGA‑TTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATAc < 1:321245/143‑1 (MQ=255) | AGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATC‑AAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTAT‑GC‑T‑ACATTTGTTTGCTGGCCTGGA‑TTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATAC > NC_000913/3637513‑3637775 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |