| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,296,380:1 | +CG | 100% | intergenic (+586/+56) | gltP → / ← yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,296,380 | 1 | . | C | 93.8% | 79.0 / ‑0.1 | 32 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base C (12/17); total (12/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | NC_000913 | 4,296,380 | 2 | . | G | 93.8% | 84.7 / 0.2 | 32 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (12/18); total (12/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTT‑TA‑‑CCGCAT‑CCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCA > NC_000913/4296300‑4296512 || cTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTT‑TACGCCGCAT‑CCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAc < 2:400898/143‑1 (MQ=2) gaCGCGTCTTATCAGGTCTTCGCCAGACAGCGCATGAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTT‑TACGCCGCAT‑CCGACA‑TCAATGCATGTTGCGTCGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGt < 2:822893/142‑1 (MQ=11) ccgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTT‑TACGCCGCAT‑CCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGtt < 2:27378/142‑1 (MQ=255) cgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTT‑TACGCCGCAT‑CCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTg < 1:185496/143‑1 (MQ=255) cgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTT‑TACGCCGCAT‑CCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCCTATCCGGCCTATCTTACCCGTCGGt > 2:309857/1‑143 (MQ=11) tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTT‑TACGCCGCAT‑CCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGttc > 2:394397/1‑142 (MQ=255) tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTT‑TACGCCGCAT‑CCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGCTa > 1:568930/1‑143 (MQ=255) tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTT‑TACGCCGCAT‑CCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAtt < 2:546230/143‑1 (MQ=255) tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTT‑TACGCCGCAT‑CCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAtttt < 1:453362/143‑1 (MQ=255) ccgaggCCAGCGAGCGCTACACCCCGATTCAGCCTGATTCCGTAGGTGGCGTACGT‑CGTT‑TATGCCGCAT‑CCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa < 2:804920/138‑1 (MQ=12) ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTT‑TACGCCGCAT‑CCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa < 2:545418/143‑1 (MQ=255) tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTT‑TACGCCGCAT‑CCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTCACCGTTGGCTAATTCTTCAACct > 1:502979/1‑143 (MQ=11) cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTT‑TACGCCGCAT‑CCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAActct > 2:897930/1‑143 (MQ=255) gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTT‑TACGCCGCAT‑CCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCtt < 2:894681/143‑1 (MQ=255) aGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTT‑TACGCCGCAT‑CCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTcac > 1:854839/1‑143 (MQ=255) aGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTT‑TACGCCGCAT‑CCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAccc < 1:355017/143‑1 (MQ=255) cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTT‑TACGCCGCAT‑CCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCgg > 1:775591/1‑143 (MQ=255) gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTT‑TACGCCGCAT‑CCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGt < 1:417236/143‑1 (MQ=255) cAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTT‑TACGCCGCAT‑CCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTa < 1:63714/143‑1 (MQ=255) gccctaTTTGGCGTGAGTCTGTCGGTCGGATAAGGCCGTTATG‑‑CCGCTG‑CCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa < 2:381365/138‑1 (MQ=21) ccTGATTTAGCGTGATTTTGTAGGTCGGCTAAGG‑CGTT‑TACGCCGCAT‑CCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCGATGTGAACCGCTGGTTCATTGTTCAAACTCCTCACACCCGGGAGCaagc > 2:613922/1‑141 (MQ=14) ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTT‑TACGCCGCAT‑CCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGCTGGTTAATTTTTCACACCCTTCCCACCCGCTACCAAAc > 2:172071/1‑143 (MQ=17) tttAGCGTGATTTTGTAGGTCGGATAAGG‑CGTT‑TACGCCGCAT‑CCGACA‑TCAATGCCTGATGCGACGCCTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTc < 1:687887/143‑1 (MQ=255) tAGCGTGATGTTGGAGGTCGGATAAGG‑CGTT‑TACGCCGCAT‑CCGACA‑TGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCa < 2:620202/143‑1 (MQ=21) aTTTTGTAGGTCGGATAAGG‑CGTT‑TACGCCGCAT‑CCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt < 1:235109/143‑1 (MQ=255) tGTAGGTCGGATAAGG‑CGTT‑TACGCCGCAT‑CCGACA‑TCAATGCCTGATGCGACGCGTGCCGCGTCTTATCAGGCCTATCTTAACCGTCAGCCACCTTTTCAAACTCTTCACACCCGGAATCAACCCCGTCCCTACACCTCAgg > 2:613673/1‑143 (MQ=17) tGTAGGTCGGATAAGG‑CGTT‑CACGCCGCAT‑CCTACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAgg > 2:490346/1‑143 (MQ=25) aGGTCGGATCAGG‑CGTT‑TA‑‑CGGGGTGTCGGCACTCACTGCGTGCTGCGACGCTTGCCGCGTCTTATCGGGCCTCTCTTAACAGTCGGTTAATTTTTACCACTTATCGCGCCCAATATCAAACCCTTCCATACAGCTCAGGTTc < 2:568723/143‑1 (MQ=255) gTCGGATAAGG‑CGTT‑TACGCCGCAT‑CCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGCTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCaa < 1:142992/143‑1 (MQ=255) gATAAGG‑CGTT‑TACGCCGCAT‑CCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAg < 1:322804/143‑1 (MQ=255) tAAGG‑CGTT‑TACGCCGCAT‑CCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTg > 1:417370/1‑143 (MQ=255) aGG‑CGTT‑TACGCCGCAT‑CCGACA‑CCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCa < 1:46709/143‑1 (MQ=255) || CTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTT‑TA‑‑CCGCAT‑CCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCA > NC_000913/4296300‑4296512 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |