| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 125,083 | C→T | 37.6% | Y689Y (TAC→TAT) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,083 | 0 | C | T | 37.6% | 39.0 / 43.6 | 58 | Y689Y (TAC→TAT) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (14/22); new base T (14/8); total (28/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.47e-01 | |||||||||||
TCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGC > NC_000913/124948‑125225 | tCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTa > 1:706355/1‑143 (MQ=255) cgacaTTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACACTATCACTACGCTGa > 2:628937/3‑143 (MQ=255) cacaTTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGTGCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAACAACAAGAGAACGTTTACTACTGTATCACTACGCTCaa > 2:515162/1‑143 (MQ=255) cacaTTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTATATCACTACGCTGaa < 1:115295/143‑1 (MQ=255) cacaTTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTATATCACTACGCTGaa > 1:94220/1‑143 (MQ=255) cacaTTCAGTCGCTGACTATCCCGAACTGTATCTATTAAGATCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTATATCACTACGGTgga > 1:484821/1‑141 (MQ=255) aTTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGa > 2:567208/1‑143 (MQ=255) tcgttCGCTCACGCTCCCGAACTGTAGCCCTTACGGCCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCCGGAGCGTATGTACGGTGAAAAACACGAGAACGTTTACTACTACATCACTACGCTGAACGaaa < 2:114780/139‑1 (MQ=255) tGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCCCTACGCTGAACGAAAACTAGcac > 2:932797/1‑143 (MQ=255) tGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACcac > 2:87553/1‑143 (MQ=255) cTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTAATACATCACTACGCTGAACGAAAACTACCACATg > 1:588427/1‑143 (MQ=255) tCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCg > 1:610165/1‑143 (MQ=255) tCCCGAACTGTATCTCTTACGACCCGGCTTACGCTGACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCg < 2:438565/143‑1 (MQ=255) ccGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGCCGGTCTGGGGCGTCTGTACGGTCAAAAACACGAGAACGTTTAGTACTATATCACTGCGCTGAAGAAAAGCAACCTCTTGCCgac > 2:189195/1‑141 (MQ=255) ccGAACTGTATCTCTTACGACCCGGCTTACGCCACAGGTCGTGCAGTCATCATGCATGACGGTCTGGAGCGTATAGACGGTGAAATACAAGAGACAGTTTACTACTATATCACTACGCTGAACTAAAACTACCACATGCCGGc > 2:332625/1‑143 (MQ=255) aaCTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACCTCACTACGCTGAACGAAGACTCCCACATGCCGGCAAt > 1:505877/1‑143 (MQ=255) aaCTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCGGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAAt < 1:638838/143‑1 (MQ=255) aaCTGTAGCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAAt < 1:443753/143‑1 (MQ=255) ttACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTg < 1:191415/143‑1 (MQ=255) ttACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAACGCCGGAAgggg > 2:172213/1‑141 (MQ=255) tACGACCCGGCTTACGCTTACGAAGTTGCTGGCATCTTACATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGc < 1:889526/143‑1 (MQ=255) gatccGGCTTACGCGTACGAAGTTGCTGCCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGACCGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGa < 1:395891/140‑1 (MQ=255) gACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGCCTGGAGCGTATGTCCGGTGAAAAACAAGAGAACGGTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGa < 1:52766/143‑1 (MQ=255) cccGTCTTATGCTCCCGAGGTTCCTGTGATCAGGCATGACGGTCGGGAGCGTATGTACGGGGAAAAACCAGAGAACGTTTACTACTATAGCGCTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAgg < 2:1991/143‑1 (MQ=255) cccGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTATATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAgg < 1:382074/143‑1 (MQ=255) cccGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCAAATGCCGGCCATGCCGGCAGGTGCTGAgg > 2:584821/1‑143 (MQ=255) ggCTTACGCTTACGACGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACGACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAg < 1:546421/143‑1 (MQ=255) ggCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTATATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAg < 1:603248/143‑1 (MQ=255) ttacgcttacgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTATATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTa > 1:265449/1‑143 (MQ=255) ttacgcttacgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTa < 1:550264/143‑1 (MQ=255) cgcttacgAAGTTGCTGTCAGCGCGCTTGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATcc < 2:610165/143‑1 (MQ=255) cttacgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGt > 1:399344/1‑143 (MQ=255) cgAAGTCGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAg < 1:549860/143‑1 (MQ=255) tGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTATATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTGAAGGTGTCTACa > 1:76809/1‑143 (MQ=255) tGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTATATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCGGAGGAAGGTATCCGTAAAGGTATCTACa > 1:350851/1‑143 (MQ=255) atgcatgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGGACGAAAACTACCCCTTGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGa < 2:173384/143‑1 (MQ=255) tgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTATATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTa > 2:178393/1‑143 (MQ=255) gACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTATATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTAt > 2:601500/1‑143 (MQ=255) gACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGGTTACTACATTATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTAt < 1:213185/143‑1 (MQ=255) ttggTCTGGAGCGTATGTACTGCGAAAAACAAGAGAACGTTTACTACTATATCACTACGCTGTACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTAtt < 1:471069/141‑1 (MQ=255) gTCTGGAGCGTATGTGCGGTGAAAAACAAGAGAACGTTTACTACTACCTCGCTACGCTGAACGAAAACTACCACACGCCGGCAATGCCGGCAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGaa < 2:279698/143‑1 (MQ=255) ggAGCGTATGTACGGAGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGCAAGGTGCTGACGAAGGTACCCGTAAAGGAATCTACAAACACGAAACAAATGAAgcca > 2:478122/1‑140 (MQ=255) gtttGTACGGTGAAAAACAAGAGAACGTTTAGTACTACATCCCTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCaaa < 2:676583/140‑1 (MQ=255) ggaTGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCaaa < 1:444141/141‑1 (MQ=255) tGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGt < 2:30227/143‑1 (MQ=255) aCGGTGAAAACAAAGAGAACGTTTACTACTATATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTaaa < 2:403973/143‑1 (MQ=255) aCGGTGAAAAACAAGAGAACGTTTACTACTATATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTaaa < 1:153224/143‑1 (MQ=255) tGAAACACACGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATCCCCGCAATGCCGGTAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTc < 1:34206/143‑1 (MQ=255) gAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTCAAGGTATATCTAATCTCGAGACTATTGAAGGTAGCCGAGGTAAAGTTCa > 2:412828/1‑143 (MQ=255) aaaaaCAAGAGAACGTTTACTACTATATCACTACGCTGAACGAAAACTACCACATGCCGGCAATACCGGAAGAGGCTGAGGAAGGTATCCGTAAAGGTATCTACAAAACAGAAAAGATTGGAGGTAGCAGAGGTACACTTCAg > 1:207613/1‑143 (MQ=255) aaaaaCAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGACGGTAGCAAAGGTAAAGTTCAg > 2:495700/1‑143 (MQ=255) aaGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTc > 1:609381/1‑143 (MQ=255) gagaACGTTTACTACTATATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCgg > 1:573580/1‑143 (MQ=255) gagaACGTTTACTACTACATCACTACGCAGCTTCCAGACTGCTACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACGCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCgg < 2:192643/143‑1 (MQ=255) agaACGTTTACTCCGACATCACGACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGc < 2:741896/143‑1 (MQ=255) aaCGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTc < 1:38250/143‑1 (MQ=255) ggttACTACTACAGCCCTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCgg < 2:468236/141‑1 (MQ=255) tttACTACTATATCACTACGCTGAACGAAAACGACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGCCTCCggg > 2:494223/1‑142 (MQ=255) ctactatATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTa < 1:515162/143‑1 (MQ=255) ctactacATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCACAGGTAAAGTTAAGCTACCCGTCTCCGGTTCTa > 1:904773/1‑143 (MQ=255) accacATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATc < 1:567208/140‑1 (MQ=255) ctacATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATcc < 1:565883/143‑1 (MQ=255) tacATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCt > 2:162104/1‑143 (MQ=255) cATCACTACGCTGAACGAAAACTTTGGCGCGCCAACAAAGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGc < 1:389498/143‑1 (MQ=255) | TCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGC > NC_000913/124948‑125225 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |