| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 696,328 | A→C | 12.9% | intergenic (+52/+102) | ubiF → / ← glnX | 2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol hydroxylase/tRNA‑Gln |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 696,328 | 0 | A | C | 12.9% | 105.4 / 9.7 | 51 | intergenic (+52/+102) | ubiF/glnX | 2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol hydroxylase/tRNA‑Gln |
| Reads supporting (aligned to +/- strand): ref base A (25/19); new base C (5/2); total (30/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.81e-01 | |||||||||||
CGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGA > NC_000913/696190‑696467 | cGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGACTTACAACATTGCCGGATCCGTGCCCACCGTAGGCCGGATCAGACGCGGCAGCGt > 2:501329/1‑143 (MQ=255) ttATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGGCGCa > 1:85699/1‑143 (MQ=255) aTGCGTACCCTCGGGTCAGTGGCGGCGGTGTGTGCTGGCGTGTTGAACCGTCAGGCGCTGACGTATGCGTTAGCGTTGTCGCCTTACACCATTGCCGGATGCGTGCCACCCGTAGGTCGGATAAGACGCGGCAGCGTCGCATc < 2:345126/143‑1 (MQ=255) ctcGGGTTAATGGCGGCGGAGCGTGCTGGCGCGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATGCGACGCTGCCGCGTCTTATCCGAcctacg < 1:649979/143‑6 (MQ=37) aTGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGa > 2:46094/1‑143 (MQ=255) gcggcgGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACgt > 1:605747/1‑143 (MQ=255) gcggcgGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACgt > 1:672362/1‑143 (MQ=255) cgggggAGCGTGCTGGGGTGGTGCAACGTCAGGCGGTGAAATGTGGGTTAGGGTTGTGGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACgtg < 2:585436/143‑1 (MQ=38) ggcggAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGAAgtgt > 2:119874/1‑143 (MQ=255) cggAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGGTGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCCTCAGACATTGAAGGATAAGACGGGTCa > 2:833751/1‑143 (MQ=255) gAGCGGGGTGGGGTGGTGAAACGGCAGGCGCTGAAGTATGCGTGAGGGTTGTAGCCGTACAACATTGCCGGATGCGTGCCAACCGTAGGGCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAAc < 2:200305/143‑1 (MQ=255) tGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACAGTGAAGGATAAGACGTGTCAACATCGc > 1:94069/1‑143 (MQ=255) gtgtGGAAACGTCAGGCGCGGAAATATGGGTTAGGGGTGGAGCCTTACAACAGTGCCGGATTCGTGCCAACCGTAGGGCGGAGAAGACGCGTCAGCGTCGCAGCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGAc < 1:646061/143‑1 (MQ=255) tgtTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAAGATTCCTGTATACTTGGCAACCTTAGGTTTGAGAAGACGCGGCAGCGTCGCATGCGACATTGAAGGATAAGACGTGTCAACATCGCACTCGACa > 2:920010/1‑143 (MQ=255) gtTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCATTACAACATTGCCGGATGCGTGCCAACCGTAGGGCGGATAAGACGCGGCAGCGGCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGAAAt > 2:312677/1‑143 (MQ=255) ttGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACAtt > 2:670342/1‑143 (MQ=255) ttGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACAtt > 2:287244/1‑143 (MQ=255) ttGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCAGACATTGAAGGAAAAGACGCGTCAACATCGCATTCGACAtt > 1:888572/1‑143 (MQ=255) gAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGGCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGGTAGGACGTGTCAACATCGCATTCGACATtga > 1:860003/1‑143 (MQ=255) aaaCGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGCCGGATAAGACGCGGCAGCGTCGCCCCCGACATCGAAGGCTAAGACGCGTCAACCTCGCATTCGACCTcgaa > 2:14949/1‑143 (MQ=255) aaaCGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCGTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACGTtgaa > 1:876504/1‑143 (MQ=255) tGAAGTATGCGTTAGGGTTGTCGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGaaaa < 1:345704/143‑1 (MQ=255) tGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGaaaa < 2:651647/143‑1 (MQ=255) tGTAGTCTTACAACAGTGCCGGATGCGTGCCACCCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCTGACATTGCAGGATAAGACGTGTGAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAg < 2:254180/143‑1 (MQ=39) aGCCTCACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGACAAGACGCGGCAGCGCCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGCAAAGCAAAAAGCTCGCCGAAGCGa < 1:554573/143‑1 (MQ=255) gcgtgcaaacgTTGCCGGATGGGGGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAAGGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAg < 2:729709/136‑1 (MQ=38) acaacaTTGCCGGATGCGTGCCAACCGTAGGTCGGATAAAACGCGGCAACGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACAATCAATGAACACAGAAAAGCAAAAACATCGCCCAAACGAGCtttt > 2:90816/1‑143 (MQ=255) aacaTTGCCGGATGCGTGCCCACCGTAGGTCGTATAAGACTCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCtttttt < 1:731648/143‑1 (MQ=255) aacaTTGCCGGATGCGTGCCAACCGTAGGGCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGGCAACATCGCATTCGACACTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCgttttt > 1:827697/1‑143 (MQ=255) tGCCGGAGGCGGCCCAACCGTAGGGCGGATCAGACGCGGCAGCGTCGCATCCGACGTTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAAtgt < 2:784604/143‑1 (MQ=255) tGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTggg < 1:820678/143‑1 (MQ=255) gTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTa > 2:531548/1‑143 (MQ=255) ccAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTGCGa > 1:884158/1‑143 (MQ=255) ccGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGAt > 2:314507/1‑143 (MQ=255) cGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGAtt > 1:814524/1‑143 (MQ=255) cGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTCTTTTAATGTGGCTGGGGTACGAGGAtt > 2:436238/1‑143 (MQ=255) gTAGGGCGGATGAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGGCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTc < 2:888572/143‑1 (MQ=255) aGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGa < 1:363573/143‑1 (MQ=255) ggTCGGATAAGACGCGGCAGCGGCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGaa < 1:944563/143‑1 (MQ=255) gTCGTATAAGCCGCGGCAGCGTCGCATCCGACATCGCACGTTAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAAc < 1:32191/143‑1 (MQ=255) cGGATGCGACGCTGCCGCGTCTTATCCGACATGGAAGGATAAGACGTGTCAACATCGCATTCGACATTGCATGCACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGagact > 2:490497/1‑139 (MQ=255) ggtgaGACGCGGCAGCGGCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCg < 1:585402/139‑1 (MQ=255) gatgcgACGCTGCCGCGTCTTATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCg > 2:889825/6‑143 (MQ=255) gatgcgACGCTGCCGCGTCTTATCCGACATGGAAGGATAAGACGTGTAAACATAGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCCTTATTAATGTGGCTGGGGTACGATGCAGCGAACCTCg > 2:71171/6‑143 (MQ=255) gatgcgACGCTGCCGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCg > 1:537814/6‑143 (MQ=255) gACGCTGCCGCGTCTTATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGAAAGAGGATACGAACCTCGGaaaa > 2:921375/1‑141 (MQ=255) aCGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGc > 1:321276/1‑143 (MQ=255) aCGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGACGATTCGAACCTCGGAATGc > 1:945175/1‑143 (MQ=255) cgcgGCCGCGTCGCATCCGACGTTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGcc < 2:672362/143‑1 (MQ=255) cgcgGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGcc < 2:591306/143‑1 (MQ=255) ggCAGCCTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGa < 2:250073/143‑1 (MQ=255) | CGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGA > NC_000913/696190‑696467 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |