Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 710,170 C→T 9.6% intergenic (+54/+30) chiQ → / ← fur lipoprotein ChiQ/DNA‑binding transcriptional dual regulator Fur

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913710,1700CT 9.6% 132.1 / 6.1 54intergenic (+54/+30)chiQ/furlipoprotein ChiQ/DNA‑binding transcriptional dual regulator Fur
Reads supporting (aligned to +/- strand):  ref base C (26/22);  new base T (2/3);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.94e-01

TCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCGGCA  >  NC_000913/710029‑710305
                                                                                                                                              |                                                                                                                                       
tcggagTGCAAAGCAAACCGGAAATGACCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGATAGCTGTAGCTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCg                                                                                                                                        <  1:259731/138‑1 (MQ=255)
tCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCCGTAATAACAAATAAGTGAGAGCAGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCg                                                                                                                                        >  2:829219/1‑143 (MQ=255)
        cAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTgg                                                                                                                                >  1:477657/1‑143 (MQ=255)
        cAACGCAAACCGGAAATAATCAGGCGGTGAAAGCCGACTGTGATACAGTCTGGCAGGAAAT‑ACGCAGTAATCACAAATAAATGAGAGCTGTAAGTCTCGCTTTGCTTATTCACCACGCACAAACACGACAAGCGGCAGGTCgg                                                                                                                                >  2:891528/1‑143 (MQ=255)
          aCGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCt                                                                                                                              <  1:113524/143‑1 (MQ=255)
          aCGCAAACCGGAAATGAGCGGGCGGTGGAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCt                                                                                                                              <  2:297776/143‑1 (MQ=255)
          aCGCAAACCGGAAATGACCAGGCCGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATCTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCt                                                                                                                              <  2:107709/143‑1 (MQ=255)
           cGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCGCGCTTTGCTTATGTAACAAACAAATATTTGTCAACCCGCAGGTTGGCtt                                                                                                                             >  1:840278/1‑143 (MQ=255)
            gCAAACCGGAAATGATCAGGCGGTCAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCGTGCATAAAAAAGCCAACCTGCGGGTTGGCttt                                                                                                                            <  2:496154/143‑1 (MQ=255)
             cAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGCCtttt                                                                                                                           >  2:750682/1‑143 (MQ=255)
             cAAACCGGAAATGAGCAGTCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAAGAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCtttt                                                                                                                           <  1:176673/143‑1 (MQ=255)
              aaaCCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtc                                                                                                                          <  1:812316/143‑1 (MQ=255)
               aaCCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtct                                                                                                                         >  1:220415/1‑143 (MQ=255)
               aaCCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtct                                                                                                                         >  2:266569/1‑143 (MQ=255)
                       gtGATCAGGCGGTCACAGCCGATTGTGATCAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAgg                                                                                                                 <  2:196180/142‑1 (MQ=255)
                               gCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCCGGTTGGCTTTTCTCGTTCAGGCTGGCTTa                                                                                                         >  1:650441/1‑143 (MQ=255)
                               gCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTa                                                                                                         >  1:200853/1‑143 (MQ=255)
                               gCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTGTCTTATTTCCCCTGCTTAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTa                                                                                                         >  1:735688/1‑143 (MQ=255)
                                 ggTGAAAGCCGATTGTGATAAAATAT‑GCATTTATTGGCGCTCGAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAGAACAGCCAACCCGCAGGTTCGCTTTTCTCGTTCAGGCTGGCTTact                                                                                                       >  2:925154/1‑141 (MQ=255)
                                       aGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATCACAAATAAGTGAGAGGTGTAACTCTCGCTGTTCTTATTTCTCTTGCATACAAAAGACAACCCGCTGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTAGCCtt                                                                                                 >  2:347126/1‑143 (MQ=255)
                                        gCCTATTGTGATTAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATATGTGATAGCTGTAACTGTCGCGTTTCTTATTTCCCTTGCATAAAAAAGCCATCCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTc                                                                                                <  2:783589/143‑1 (MQ=255)
                                        gCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTc                                                                                                >  2:427615/1‑143 (MQ=255)
                                               gtgATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCa                                                                                         <  1:904119/143‑1 (MQ=255)
                                               gtgATAAAGTCGGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCa                                                                                         <  2:309783/143‑1 (MQ=255)
                                                 gATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCGGGTTGGCTTTTCTCGTTCAGTCTGGCTTATTTGCCTTCGTGCGCATg                                                                                       >  1:704670/1‑143 (MQ=255)
                                                  aTAAAGTCTGTCGGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTAGTTGCCTTCGTGCGCATGt                                                                                      >  2:719687/1‑143 (MQ=255)
                                                      aGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCAt                                                                                  <  2:118080/143‑1 (MQ=255)
                                                           ggCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTcg                                                                             <  1:158521/143‑1 (MQ=255)
                                                           ggCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTcg                                                                             <  2:719150/143‑1 (MQ=255)
                                                            gCACGAAAT‑ACGCAGCAATAACAAGTAAGGGAGAGCTGTAACTCTCGCTGTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCGTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTcgc                                                                            <  2:300095/143‑1 (MQ=255)
                                                             cAGGAAAT‑ACGCAGTAATAACAAATGAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGGTGGCTTTTCGCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTcgcg                                                                           <  1:585908/143‑1 (MQ=255)
                                                             cAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTGATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTcgcg                                                                           <  2:399334/143‑1 (MQ=255)
                                                                  aaT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCCGGCTGGCTTATTTGCCTTCGTGCGCATTTTCATCTTCGCTGCAAt                                                                      >  1:240598/1‑143 (MQ=255)
                                                                    t‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGACTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCg                                                                    >  1:914666/1‑143 (MQ=255)
                                                                                aaCAAATGAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGcaca                                                         <  1:648039/143‑1 (MQ=255)
                                                                                aaCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTAttt                                                                                                      >  1:378213/1‑98 (MQ=255)
                                                                                aaCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTAttt                                                                                                      <  2:378213/98‑1 (MQ=255)
                                                                                    actaggTGAGCCCTGTAACTCTCCCTATACTTCTTGCCCTTGCATAAAACAGCCAACCCCCAGGTTGGCGTTTCTCGTTCAGGCTCGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGa                                                     <  1:31048/138‑1 (MQ=255)
                                                                                     aTAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGTCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGAc                                                    <  1:419530/143‑1 (MQ=255)
                                                                                           gagaGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCTCTGGTTGGCTTTTCTTGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTaaa                                              <  1:36643/143‑1 (MQ=255)
                                                                                                cTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATag                                         <  1:216792/143‑1 (MQ=255)
                                                                                                         tcGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTgg                                <  2:38891/143‑1 (MQ=255)
                                                                                                           gCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTACCGATAGAGTCTGTGCtt                              >  1:114241/1‑143 (MQ=255)
                                                                                                           gCTTTTCTTATTTCCCTTGCATAAAAAAGCAAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCCCAGTGACCGTACAGATCGAGACTGTGGtc                              >  2:512858/1‑142 (MQ=255)
                                                                                                               ttCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACTGTATAGATAGAGACTGTGGTTAGTc                          >  2:724764/1‑143 (MQ=255)
                                                                                                                 cTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCCTCTTCGCGGCAATCGCCTTCGGCACAGTGGCCGTACAGATAGATACTGTGGTTAGTGAg                        >  2:461173/1‑143 (MQ=255)
                                                                                                                  ttATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAgg                       >  1:508529/1‑143 (MQ=255)
                                                                                                                    aTTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTCTTTGCCTTCGTGCGCATGTTCATCTTCGCGGAAACCGCCTTTGGCTCAGTGGTCGTAACCATACAGACTCTGCTTAGTCAGGCg                     >  1:586470/1‑143 (MQ=255)
                                                                                                                           ttGCATAACAGAGCCAACCCGCAGGTTGGCTTTGCTCGTTCAGGCTGGCTTATTTGCCTTCGGGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCa              <  2:28473/143‑1 (MQ=255)
                                                                                                                           ttGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCa              >  1:878948/1‑143 (MQ=255)
                                                                                                                                tAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTGGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGttt         >  1:13681/1‑143 (MQ=255)
                                                                                                                                   aaaaGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGc      >  1:877631/1‑143 (MQ=255)
                                                                                                                                    aaaGCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCg     >  1:169855/1‑143 (MQ=255)
                                                                                                                                       gCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTCGCCTTCGTGCGCATGTTCATCTTCGCGGCACTCGCCTTCGGCACAGTGACCGTAAAGATAGCGCCGGTGGTTAGTCAGGCGCATGCCACGCTTAGCGGCa  >  2:325684/1‑143 (MQ=255)
                                                                                                                                              |                                                                                                                                       
TCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAAT‑ACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCGGCA  >  NC_000913/710029‑710305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑